| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,879,631 | T→C | 37.6% | E179E (GAA→GAG) | mazG ← | nucleoside triphosphate pyrophosphohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,879,631 | 0 | T | C | 37.6% | 60.8 / 70.6 | 109 | E179E (GAA→GAG) | mazG | nucleoside triphosphate pyrophosphohydrolase |
| Reads supporting (aligned to +/- strand): ref base T (43/25); new base C (20/21); total (63/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 109 total aligned reads displayed.
AACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCG > minE/1879572‑1879697 | aagagcagGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATc > 1:351312/4‑69 (MQ=255)aaCAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCTTGCCGCGCTTCGTACATc > 1:1982335/1‑69 (MQ=255)aaCAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATc > 1:2257717/1‑69 (MQ=255)aaCAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATc > 1:223439/1‑69 (MQ=255)aaCAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATc > 1:2224375/1‑69 (MQ=255)aaCAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATc > 1:2219855/1‑69 (MQ=255)aaCAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATc < 1:1587960/69‑1 (MQ=255)aaCAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATc > 1:265743/1‑69 (MQ=255)aaCAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATc > 1:1931530/1‑69 (MQ=255)aaCAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATc > 1:3023798/1‑69 (MQ=255)aaCAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATc > 1:3019809/1‑69 (MQ=255) ggTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCtcg > 1:1534617/1‑68 (MQ=255) aTTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCCTCGTACATCACCtcgt > 1:1466496/1‑61 (MQ=255) aTTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCCTCGTACATCACCtcgt > 1:156772/1‑61 (MQ=255) aTTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCCTCGTACATCACCtcgt > 1:3110190/1‑61 (MQ=255) aTTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCCTCGTACATCACCtcgt > 1:2924432/1‑61 (MQ=255) aTTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCCTCGTACATCACCtcgt > 1:1928440/1‑61 (MQ=255) aTTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCCTCGTACATCACCtcgt > 1:2006282/1‑61 (MQ=255) aTTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCCTCGTACATCACCtcgt > 1:2039251/1‑61 (MQ=255) aTTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCCTCGTACATCACCtcgt > 1:2526795/1‑61 (MQ=255) aTTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCCTCGTACATCACCtcgt > 1:2502357/1‑61 (MQ=255) aTTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCCTCGTACATCACCtcgt > 1:2296974/1‑61 (MQ=255) aTTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCCTCGTACATCACCtcgt > 1:1007911/1‑61 (MQ=255) aTTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCCTCGTACATCACCtcgt > 1:3327470/1‑61 (MQ=255) aTTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCCTCGTACATCACCtcgt > 1:1019144/1‑61 (MQ=255) aTTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCCTCGTACATCACCtcgt > 1:3383776/1‑61 (MQ=255) aTTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCCTCGTACATCACCtcgt > 1:828266/1‑61 (MQ=255) aTTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCCTCGTACATCACCtcgt > 1:1203352/1‑61 (MQ=255) aTTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCCTCGTACATCACCtcgt > 1:385261/1‑61 (MQ=255) aTTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCCTCGTACATCACCtcgt > 1:810567/1‑61 (MQ=255) aTTTCATCCTCCAGTTTAGCCTGGTCGACAACAGACTGCCGCGCCTCGTACATCACCtcgt > 1:660592/1‑61 (MQ=255) tcctcctccAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTc > 1:1053850/1‑44 (MQ=255) tcctcctccAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTAc > 1:2644166/1‑48 (MQ=255) tcctcctccAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAt > 1:811491/1‑62 (MQ=255) tcctcctccAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAt > 1:1318063/1‑62 (MQ=255) tcctcctccAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAt > 1:1928949/1‑62 (MQ=255) tcctcctccAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAt > 1:1371387/1‑62 (MQ=255) tcctcctccAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAt > 1:2657113/1‑62 (MQ=255) cctccAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTACGTACATCACCTCGTCGATCTCTTCGTAGa > 1:1255438/1‑69 (MQ=255) ctccAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGAc > 1:1261088/1‑69 (MQ=255) ccAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGAc < 1:2597432/67‑1 (MQ=255) ccAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGAc < 1:2182377/67‑1 (MQ=255) gTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACttt < 1:1302232/67‑1 (MQ=255) tAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCtcgtcg > 1:1428773/1‑47 (MQ=255) tAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCtcgtcg > 1:1297293/1‑47 (MQ=255) tAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCtcgtcg > 1:1106758/1‑47 (MQ=255) tAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCtcgtcg > 1:2946784/1‑47 (MQ=255) tAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCtcgtc > 1:206575/1‑46 (MQ=255) tAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATc > 1:2899427/1‑67 (MQ=255) tAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATc > 1:3008250/1‑67 (MQ=255) tAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATc < 1:361821/67‑1 (MQ=255) tAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATc > 1:1013505/1‑67 (MQ=255) tAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATc < 1:3286069/67‑1 (MQ=255) tAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATc < 1:3289947/67‑1 (MQ=255) tAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATc > 1:213582/1‑67 (MQ=255) tAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATc > 1:1182135/1‑67 (MQ=255) tAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATc < 1:1976044/67‑1 (MQ=255) tAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATc > 1:1422707/1‑67 (MQ=255) tAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATc < 1:1411230/67‑1 (MQ=255) tAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATc > 1:1860309/1‑67 (MQ=255) tAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATc > 1:1774443/1‑67 (MQ=255) tAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCg > 1:389998/1‑68 (MQ=255) tAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCg > 1:1510629/1‑68 (MQ=255) tAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCg > 1:73486/1‑68 (MQ=255) ccTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACt < 1:3162627/68‑1 (MQ=255) ccTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACt < 1:3052668/68‑1 (MQ=255) ccTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACt < 1:1632967/68‑1 (MQ=255) ccTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACt < 1:2770436/68‑1 (MQ=255) ccTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACt < 1:78396/68‑1 (MQ=255) ccTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACt < 1:2222302/68‑1 (MQ=255) ccTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACt < 1:1098019/68‑1 (MQ=255) cTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATc < 1:1316920/63‑1 (MQ=255) gACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGa > 1:1650753/1‑67 (MQ=255) gACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGa > 1:1100994/1‑67 (MQ=255) ccGCGCCTCGTACATCACCTCGTCGATCTCTTCGTAGACtt < 1:824218/41‑1 (MQ=255) ccGCGCCTCGTACATCACCTCGTCGATCTCTTCGTAGACtt < 1:51184/41‑1 (MQ=255) ccGCGCCTCGTACATCACCTCGTCGATCTCTTCGTAGACtt < 1:457312/41‑1 (MQ=255) ccGCGCCTCGTACATCACCTCGTCGATCTCTTCGTAGACtt < 1:649243/41‑1 (MQ=255) ccGCGCCTCGTACATCACCTCGTCGATCTCTTCGTAGACtt < 1:438930/41‑1 (MQ=255) ccGCGCCTCGTACATCACCTCGTCGATCTCTTCGTAGACtt < 1:3207826/41‑1 (MQ=255) ccGCGCCTCGTACATCACCTCGTCGATCTCTTCGTAGACtt < 1:750027/41‑1 (MQ=255) ccGCGCCTCGTACATCACCTCGTCGATCTCTTCGTAGACtt < 1:762496/41‑1 (MQ=255) ccGCGCCTCGTACATCACCTCGTCGATCTCTTCGTAGACtt < 1:1067494/41‑1 (MQ=255) ccGCGCCTCGTACATCACCTCGTCGATCTCTTCGTAGACtt < 1:1026603/41‑1 (MQ=255) ccGCGCCTCGTACATCACCTCGTCGATCTCTTCGTAGACtt < 1:854568/41‑1 (MQ=255) ccGCGCCTCGTACATCACCTCGTCGATCTCTTCGTAGACtt < 1:3203311/41‑1 (MQ=255) ccGCGCCTCGTACATCACCTCGTCGATCTCTTCGTAGACtt < 1:1560619/41‑1 (MQ=255) ccGCGCCTCGTACATCACCTCGTCGATCTCTTCGTAGACtt < 1:865698/41‑1 (MQ=255) ccGCGCCTCGTACATCACCTCGTCGATCTCTTCGTAGACtt < 1:1944750/41‑1 (MQ=255) ccGCGCCTCGTACATCACCTCGTCGATCTCTTCGTAGACtt < 1:1951763/41‑1 (MQ=255) ccGCGCCTCGTACATCACCTCGTCGATCTCTTCGTAGACtt < 1:1996213/41‑1 (MQ=255) ccGCGCCTCGTACATCACCTCGTCGATCTCTTCGTAGACtt < 1:2314004/41‑1 (MQ=255) ccGCGCCTCGTACATCACCTCGTCGATCTCTTCGTAGACtt < 1:2280306/41‑1 (MQ=255) ccGCGCCTCGTACATCACCTCGTCGATCTCTTCGTAGACtt < 1:2333034/41‑1 (MQ=255) gcTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCg < 1:918191/69‑1 (MQ=255) gcTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCg < 1:605721/69‑1 (MQ=255) gcTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCg < 1:1536637/69‑1 (MQ=255) gcTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCg < 1:1721504/69‑1 (MQ=255) gcTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCg < 1:2604443/69‑1 (MQ=255) cTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCg < 1:1427935/68‑1 (MQ=255) | AACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCG > minE/1879572‑1879697 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |