| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,879,862 | A→G | 42.5% | D102D (GAT→GAC) | mazG ← | nucleoside triphosphate pyrophosphohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,879,862 | 0 | A | G | 42.5% | 7.1 / 27.7 | 40 | D102D (GAT→GAC) | mazG | nucleoside triphosphate pyrophosphohydrolase |
| Reads supporting (aligned to +/- strand): ref base A (18/5); new base G (5/12); total (23/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
CCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGT > minE/1879822‑1879929 | cccAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGct < 1:350179/69‑1 (MQ=255)cccAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGct < 1:2014552/69‑1 (MQ=255)cccAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAAATATCAGCAAAAACATGCGGATGGCGACGct < 1:1941502/69‑1 (MQ=255) cAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCg > 1:51137/1‑48 (MQ=255) cAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTAt > 1:746175/1‑69 (MQ=255) cAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTAt > 1:597518/1‑69 (MQ=255) cACTTCACTGCTATTTTCGGCAGAACTGTCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCt < 1:1933781/69‑1 (MQ=255) cACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCt > 1:1086473/1‑69 (MQ=255) cACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCt > 1:652492/1‑69 (MQ=255) cACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCt > 1:451952/1‑69 (MQ=255) cACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCt < 1:3279398/69‑1 (MQ=255) cACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCt > 1:3104600/1‑69 (MQ=255) cACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCt > 1:2755380/1‑69 (MQ=255) cACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCt < 1:2140445/69‑1 (MQ=255) cACTTCACTACTATTTTCGGCAGAACTGTCAGCAAAAACATGCGGATGGCGACTCTCTAATTTATCGCt < 1:3273587/69‑1 (MQ=255) cACTTCACTACTATTTTCGGCAGAACTGTCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCt < 1:13007/69‑1 (MQ=255) cACTTCACTACTATTTTCGGCAGAACTGTCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCt < 1:1547142/69‑1 (MQ=255) cACTTCACTACTATTTTCGGCAGAACTGTCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCt < 1:387827/69‑1 (MQ=255) cACTTCACTACTATTTTCGGCAGAACTGTCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCt < 1:36822/69‑1 (MQ=255) cACTTCACTACTATTTTCGGCAGAACTGTCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCt < 1:3364389/69‑1 (MQ=255) cACTTCACTACTATTTTCGGCAGAACTGTCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCt < 1:3185486/69‑1 (MQ=255) cACTTCACTACTATTTTCGGCAGAACTGTCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCt < 1:1822119/69‑1 (MQ=255) cACTTCACTACTATTTTCGGCAGAACTGTCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCt < 1:2962829/69‑1 (MQ=255) cACTTCACTACTATTTTCGGCAGAACTGTCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCt < 1:182265/69‑1 (MQ=255) cACTTCACTACTATTTTCGGCAGAACTGTCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCt < 1:1956839/69‑1 (MQ=255) cACTTAACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATAGCt > 1:499193/1‑69 (MQ=255) cACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAAt > 1:2385160/1‑67 (MQ=255) tttCGGCAGAACTGTCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCaaa > 1:739096/1‑69 (MQ=255) tttCGGCAGAACTGTCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCaaa > 1:2626338/1‑69 (MQ=255) tttCGGCAGAACTGTCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCaaa > 1:2503387/1‑69 (MQ=255) tttCGGCAGAACTGTCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCaaa > 1:1643216/1‑69 (MQ=255) tttCGGCAGAACTGTCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCaaa > 1:1876918/1‑69 (MQ=255) ttCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAat > 1:1759002/1‑69 (MQ=255) ttCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAat > 1:1905530/1‑69 (MQ=255) aGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCAt > 1:1080597/1‑68 (MQ=255) aGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCAt > 1:3265134/1‑68 (MQ=255) aCTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATagcag > 1:246256/1‑53 (MQ=255) cTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTaa > 1:1447606/1‑46 (MQ=255) cTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTaaa > 1:1299236/1‑68 (MQ=255) cTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTaaa > 1:3012520/1‑68 (MQ=255) aTCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGt > 1:302432/1‑68 (MQ=255) | CCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGT > minE/1879822‑1879929 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |