| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,881,919 | C→T | 45.5% | R405R (CGG→CGA) | relA ← | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,881,919 | 0 | C | T | 45.5% | 14.2 / 63.6 | 79 | R405R (CGG→CGA) | relA | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Reads supporting (aligned to +/- strand): ref base C (20/23); new base T (18/18); total (38/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
GTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCT > minE/1881856‑1881979 | gTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGgtc > 1:192451/1‑69 (MQ=255)gTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGgtc > 1:492320/1‑69 (MQ=255)gTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGgtc > 1:1143312/1‑69 (MQ=255)gTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGgtc > 1:389930/1‑69 (MQ=255)gTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGgtc > 1:3423955/1‑69 (MQ=255)gTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGgtc > 1:977664/1‑69 (MQ=255)gTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGgtc > 1:3246877/1‑69 (MQ=255)gTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGgt > 1:2991033/1‑68 (MQ=255) cAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGgtcgt > 1:2715925/1‑68 (MQ=255) cAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGgtcgt > 1:2983126/1‑68 (MQ=255) cAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGgtcgt > 1:9848/1‑68 (MQ=255) cAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGgtcgt > 1:2244316/1‑68 (MQ=255) aTCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACTCGgtcgt > 1:2877701/1‑57 (MQ=255) aTCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACTCGGTCGTCAAAGACCt > 1:3233522/1‑66 (MQ=255) aTCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACTCGGTCGTCAAAGACCt > 1:1994989/1‑66 (MQ=255) aTCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACTCGGTCGTCAAAGACCTg > 1:2570011/1‑67 (MQ=255) aTCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACTCGGTCGTCAAAGACCTg > 1:2694776/1‑67 (MQ=255) aTCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACTCGGTCGTCAAAGACCTg > 1:222949/1‑67 (MQ=255) aTCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACTCGGTCGTCAAAGACCTg > 1:2734550/1‑67 (MQ=255) aTCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACTCGGTCGTCAAAGACCTg > 1:274759/1‑67 (MQ=255) aTCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACTCGGTCGTCAAAGACCTg > 1:3340596/1‑67 (MQ=255) aTCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACTCGGTCGTCAAAGACCTg > 1:3417924/1‑67 (MQ=255) aTCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACTCGGTCGTCAAAGACCTg > 1:606118/1‑67 (MQ=255) aTCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACTCGGTCGTCAAAGACCTg > 1:2202559/1‑67 (MQ=255) aTCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACTCGGTCGTCAAAGACCTg > 1:2192827/1‑67 (MQ=255) aTCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACTCGGTCGTCAAAGACCTg > 1:1111236/1‑67 (MQ=255) aTCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACTCGGTCGTCAAAGACCTg > 1:218868/1‑67 (MQ=255) aTCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACTCGGTCGTCAAAGACATg > 1:3083662/1‑67 (MQ=255) aTCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACTCGCTCGTCAAAGACCt > 1:2206471/1‑66 (MQ=255) aTCCCGCAGCCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACTCGGTCGTCAAAGACCTg > 1:1892270/1‑67 (MQ=255) aCCTTTCGGCGTAAAGACGTACACCCGGTCGTGAAAGACCTGACTACGTa > 1:2866447/1‑50 (MQ=255) aCCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAATACCt > 1:1895904/1‑41 (MQ=255) aCCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGCCCTGACTACGTACTTCGTCGAGCATTTCGc > 1:2250040/1‑68 (MQ=255) aCCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGc > 1:699217/1‑68 (MQ=255) aCCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGc > 1:1887279/1‑68 (MQ=255) tttCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGa < 1:831561/69‑1 (MQ=255) ttCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGa < 1:520273/68‑1 (MQ=255) gCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCa < 1:2120621/68‑1 (MQ=255) gCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCa < 1:2019903/68‑1 (MQ=255) gCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCACCGGAATCa < 1:3302190/68‑1 (MQ=255) aggTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATctct < 1:296545/67‑1 (MQ=255) aCGTACACTCGGTCGTCATAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATatct < 1:369976/69‑1 (MQ=255) aCGTACACTCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATatct < 1:332546/69‑1 (MQ=255) aCGTACACTCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATatct < 1:3300748/69‑1 (MQ=255) aCGTACACTCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATatct < 1:1573909/69‑1 (MQ=255) aCGTACACTCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATatct < 1:3134703/69‑1 (MQ=255) aCGTACACTCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATatct < 1:693169/69‑1 (MQ=255) aCGTACACTCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATatct < 1:511969/69‑1 (MQ=255) aCGTACACTCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATatct < 1:555692/69‑1 (MQ=255) aCGTACACTCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATatct < 1:648391/69‑1 (MQ=255) aCGTACACTCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATatct < 1:1092765/69‑1 (MQ=255) aCGTACACTCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATatct < 1:3180601/69‑1 (MQ=255) aCGTACACTCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATatct < 1:2416532/69‑1 (MQ=255) aCGTACACTCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATatct < 1:3096268/69‑1 (MQ=255) aCGTACACTCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATatct < 1:2443209/69‑1 (MQ=255) aCGTACACTCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATatct < 1:2492108/69‑1 (MQ=255) aCGTACACTCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATatct < 1:891205/69‑1 (MQ=255) aCGTACACTCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATatct < 1:2103647/69‑1 (MQ=255) aCGTACACTCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATatct < 1:191018/69‑1 (MQ=255) aCGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATctct < 1:2363502/69‑1 (MQ=255) aCGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATctct < 1:1766339/69‑1 (MQ=255) aCGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATctct < 1:13777/69‑1 (MQ=255) aCGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATctct < 1:1435057/69‑1 (MQ=255) aCGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATctct < 1:1530803/69‑1 (MQ=255) aCGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATctct < 1:2316603/69‑1 (MQ=255) aCGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATctct < 1:3233602/69‑1 (MQ=255) cacaCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATctct > 1:3435749/1‑64 (MQ=255) cacCCGGTCTTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATctct < 1:285962/64‑1 (MQ=255) cacCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATctct < 1:1027822/64‑1 (MQ=255) cacCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATctct < 1:998577/64‑1 (MQ=255) cacCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATctct < 1:685612/64‑1 (MQ=255) cacCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATctct < 1:603553/64‑1 (MQ=255) cacCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATctct < 1:441521/64‑1 (MQ=255) cacCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATctct < 1:3027342/64‑1 (MQ=255) cacCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATctct < 1:286503/64‑1 (MQ=255) cacCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATctct < 1:2615441/64‑1 (MQ=255) cacCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATctct > 1:234621/1‑64 (MQ=255) cacCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATctct > 1:1481399/1‑64 (MQ=255) cacCCGGTCGTCAAAGACCTGACGACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATctct > 1:1236339/1‑64 (MQ=255) cacCCCGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATctct < 1:505735/64‑1 (MQ=255) | GTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCT > minE/1881856‑1881979 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |