| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,882,378 | A→T | 17.7% | R252R (CGT→CGA) | relA ← | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,882,378 | 0 | A | T | 17.7% | 199.7 / 30.5 | 101 | R252R (CGT→CGA) | relA | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Reads supporting (aligned to +/- strand): ref base A (25/58); new base T (12/6); total (37/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.03e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 109 total aligned reads displayed.
AAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGA > minE/1882312‑1882439 | aaaCAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGgacg > 1:1990615/1‑69 (MQ=255)aaaCAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGgacg > 1:2301953/1‑69 (MQ=255)aaaCAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGgacg > 1:253589/1‑69 (MQ=255)aaaCAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGgacg > 1:2616926/1‑69 (MQ=255)aaaCAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGgacg > 1:1273125/1‑69 (MQ=255)aaaCAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGgacg > 1:2628525/1‑69 (MQ=255)aaaCAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGgacg > 1:1568022/1‑69 (MQ=255) cTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATa < 1:2687033/69‑1 (MQ=255) cTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATa < 1:1185577/69‑1 (MQ=255) caAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATacac > 1:876905/1‑67 (MQ=255) gAGGTTCTTTTTCTGCATTTTACGCCATATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:2534461/69‑1 (MQ=255) gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:1440192/69‑1 (MQ=255) gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:45524/69‑1 (MQ=255) gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:1467626/69‑1 (MQ=255) gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:1626772/69‑1 (MQ=255) gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:825567/69‑1 (MQ=255) gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:2491062/69‑1 (MQ=255) gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:1942484/69‑1 (MQ=255) gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:1423512/69‑1 (MQ=255) gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:1322730/69‑1 (MQ=255) gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:2982653/69‑1 (MQ=255) gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:3160928/69‑1 (MQ=255) gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:1123705/69‑1 (MQ=255) aGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:2839903/68‑1 (MQ=255) cTTTTTCTGCATTTTAGGCCAGATGCTGTAGATGTGTTTCGGACGACCATa > 1:1560124/1‑51 (MQ=255) cTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCAt > 1:2044405/1‑50 (MQ=255) cTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATa > 1:1950669/1‑51 (MQ=255) cTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATa > 1:1924082/1‑51 (MQ=255) cTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATa > 1:715573/1‑51 (MQ=255) cTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATa > 1:1099655/1‑51 (MQ=255) cTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATa > 1:111086/1‑51 (MQ=255) cTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATa > 1:1037081/1‑51 (MQ=255) cTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATa > 1:2977402/1‑51 (MQ=255) cTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATa > 1:1297442/1‑51 (MQ=255) cTTTTTCTGCATTTTACGCCAGATGCCGTAGATGTGTTTCGGACGACCATa > 1:2637611/1‑51 (MQ=255) tttCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:3219854/60‑1 (MQ=255) tCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCa < 1:2200608/68‑1 (MQ=255) tCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCa < 1:2900846/68‑1 (MQ=255) tCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCa < 1:643976/68‑1 (MQ=255) tCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCa < 1:1078158/68‑1 (MQ=255) tCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCa < 1:196666/68‑1 (MQ=255) tCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCa < 1:1710023/68‑1 (MQ=255) tCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCa < 1:809253/68‑1 (MQ=255) tCTGAATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGAACATACACTTCCGCTTTAACGCCTTCa < 1:2664282/68‑1 (MQ=255) cTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAg < 1:2521838/68‑1 (MQ=255) cTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAg < 1:3031318/68‑1 (MQ=255) cTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAg < 1:688625/68‑1 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTc < 1:2172306/68‑1 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTc < 1:1082936/68‑1 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTc < 1:199599/68‑1 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCa > 1:2781318/1‑69 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCa > 1:247757/1‑69 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCa > 1:2714847/1‑69 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCa > 1:1578815/1‑69 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCa > 1:1583189/1‑69 (MQ=255) ttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATacac > 1:1136484/1‑41 (MQ=255) ttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCAt > 1:2252410/1‑68 (MQ=255) ttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCAt > 1:2415964/1‑68 (MQ=255) ttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCAt > 1:1484043/1‑68 (MQ=255) ttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCAt > 1:2563348/1‑68 (MQ=255) ccAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAgc > 1:3351888/1‑69 (MQ=255) ccAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAgc > 1:3315393/1‑69 (MQ=255) gATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:3281604/42‑1 (MQ=255) aTGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAg < 1:1875347/52‑1 (MQ=255) tGCTGTAGATGTGTTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCa < 1:2594890/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCa < 1:863065/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCa < 1:531738/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCa < 1:2499759/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCa < 1:1429997/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:3024413/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:1882531/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:1278626/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:1285844/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:875258/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:262711/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:1744129/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:2312177/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:175995/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:1878815/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTACGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:2426769/69‑1 (MQ=255) gCTGTAGATGTGTTTCGGTCGACCATACACTTCAGCTTTAACACCTACAGCTTTCATCTCAGCGCGCa < 1:1157505/68‑1 (MQ=255) gCTGTAGATGTGTTTCGGACGACCATAGACTTCCGCTTTAACGCCTTCa < 1:2582384/49‑1 (MQ=255) gtgtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:965747/59‑1 (MQ=255) gtgtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:622342/59‑1 (MQ=255) gtgtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:969798/59‑1 (MQ=255) gtgtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:3369300/59‑1 (MQ=255) gtgtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:2339363/59‑1 (MQ=255) gtgtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:1625744/59‑1 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:3423460/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:3396844/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:3334062/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:2025154/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:163754/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:1486866/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:942004/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:1224723/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAca > 1:3308541/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAca > 1:1220239/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCAACTCAGCGCGCAGATGGCCGAcaa > 1:1750404/1‑67 (MQ=255) acgacCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCa < 1:1371645/58‑1 (MQ=255) | AAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGA > minE/1882312‑1882439 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |