| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,882,393 | C→A | 19.3% | A247A (GCG→GCT) | relA ← | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,882,393 | 0 | C | A | 19.3% | 188.4 / 28.5 | 93 | A247A (GCG→GCT) | relA | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Reads supporting (aligned to +/- strand): ref base C (19/56); new base A (12/6); total (31/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.65e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.36e-01 | |||||||||||
GAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCG > minE/1882330‑1882459 | gAGGTTCTTTTTCTGCATTTTACGCCATATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:2534461/69‑1 (MQ=255)gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:2491062/69‑1 (MQ=255)gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:154682/69‑1 (MQ=255)gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:2982653/69‑1 (MQ=255)gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:1626772/69‑1 (MQ=255)gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:1467626/69‑1 (MQ=255)gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:1440192/69‑1 (MQ=255)gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:3160928/69‑1 (MQ=255)gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:1423512/69‑1 (MQ=255)gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:1322730/69‑1 (MQ=255)gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:1942484/69‑1 (MQ=255)gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:45524/69‑1 (MQ=255)gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:268112/69‑1 (MQ=255)gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:825567/69‑1 (MQ=255)gAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:1123705/69‑1 (MQ=255) aGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:635906/68‑1 (MQ=255) aGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:2839903/68‑1 (MQ=255) tttCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:3219854/60‑1 (MQ=255) tCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCa < 1:1710023/68‑1 (MQ=255) tCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCa < 1:2200608/68‑1 (MQ=255) tCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCa < 1:1078158/68‑1 (MQ=255) tCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCa < 1:2900846/68‑1 (MQ=255) tCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCa < 1:643976/68‑1 (MQ=255) tCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCa < 1:809253/68‑1 (MQ=255) tCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCa < 1:196666/68‑1 (MQ=255) tCTGAATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGAACATACACTTCCGCTTTAACGCCTTCa < 1:2664282/68‑1 (MQ=255) cTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAg < 1:3031318/68‑1 (MQ=255) cTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAg < 1:688625/68‑1 (MQ=255) cTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAg < 1:2521838/68‑1 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTc < 1:2172306/68‑1 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTc < 1:199599/68‑1 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTc < 1:3267771/68‑1 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTc < 1:1082936/68‑1 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCa > 1:2714847/1‑69 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCa > 1:2781318/1‑69 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCa > 1:247757/1‑69 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCa > 1:1583189/1‑69 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCa > 1:1578815/1‑69 (MQ=255) ttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCAt > 1:2563348/1‑68 (MQ=255) ttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCAt > 1:1484043/1‑68 (MQ=255) ttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCAt > 1:2252410/1‑68 (MQ=255) ttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCAt > 1:2415964/1‑68 (MQ=255) ccAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAgc > 1:3315393/1‑69 (MQ=255) ccAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAgc > 1:1276040/1‑69 (MQ=255) ccAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAgc > 1:3351888/1‑69 (MQ=255) gATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCttt < 1:3281604/42‑1 (MQ=255) aTGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAg < 1:1875347/52‑1 (MQ=255) tGCTGTAGATGTGTTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCa < 1:2499759/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCa < 1:863065/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCa < 1:531738/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCa < 1:1429997/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCa < 1:2594890/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:1285844/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:1278626/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:3024413/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:1744129/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:262711/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:175995/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:1798290/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:2312177/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:1878815/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:1882531/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:875258/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTACGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:2426769/69‑1 (MQ=255) gCTGTAGATGTGTTTCGGTCGACCATACACTTCAGCTTTAACACCTACAGCTTTCATCTCAGCGCGCa < 1:1157505/68‑1 (MQ=255) gCTGTAGATGTGTTTCGGACGACCATAGACTTCCGCTTTAACGCCTTCa < 1:2582384/49‑1 (MQ=255) gtgtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:622342/59‑1 (MQ=255) gtgtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:3369300/59‑1 (MQ=255) gtgtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:965747/59‑1 (MQ=255) gtgtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:969798/59‑1 (MQ=255) gtgtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:2339363/59‑1 (MQ=255) gtgtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:2515459/59‑1 (MQ=255) gtgtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:1625744/59‑1 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:2025154/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:2155422/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:942004/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:1224723/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:163754/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:3423460/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:3396844/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:1486866/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:3334062/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAca > 1:1220239/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAca > 1:3308541/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCAACTCAGCGCGCAGATGGCCGAcaa > 1:1750404/1‑67 (MQ=255) acgacCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCa < 1:1371645/58‑1 (MQ=255) cacTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAgtg > 1:1543971/1‑69 (MQ=255) cacTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAgtg > 1:923619/1‑69 (MQ=255) tCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTcg > 1:2114627/1‑69 (MQ=255) tCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTcg > 1:2560801/1‑69 (MQ=255) tCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTcg > 1:1205101/1‑69 (MQ=255) tCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTcg > 1:3053387/1‑69 (MQ=255) tCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTcg > 1:3196501/1‑69 (MQ=255) | GAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCG > minE/1882330‑1882459 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |