| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,882,402 | G→A | 30.7% | G244G (GGC→GGT) | relA ← | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,882,402 | 0 | G | A | 30.7% | 92.5 / 41.9 | 94 | G244G (GGC→GGT) | relA | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Reads supporting (aligned to +/- strand): ref base G (20/45); new base A (23/6); total (43/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAG > minE/1882341‑1882467 | tCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCa < 1:2200608/68‑1 (MQ=255)tCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCa < 1:1078158/68‑1 (MQ=255)tCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCa < 1:2900846/68‑1 (MQ=255)tCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCa < 1:196666/68‑1 (MQ=255)tCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCa < 1:809253/68‑1 (MQ=255)tCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCa < 1:1710023/68‑1 (MQ=255)tCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCa < 1:643976/68‑1 (MQ=255)tCTGAATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGAACATACACTTCCGCTTTAACGCCTTCa < 1:2664282/68‑1 (MQ=255) cTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAg < 1:2521838/68‑1 (MQ=255) cTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAg < 1:3031318/68‑1 (MQ=255) cTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAg < 1:688625/68‑1 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTc < 1:1082936/68‑1 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTc < 1:2172306/68‑1 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTc < 1:199599/68‑1 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTc < 1:3267771/68‑1 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCa > 1:1578815/1‑69 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCa > 1:2714847/1‑69 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCa > 1:2781318/1‑69 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCa > 1:247757/1‑69 (MQ=255) ttttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCa > 1:1583189/1‑69 (MQ=255) ttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCAt > 1:1484043/1‑68 (MQ=255) ttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCAt > 1:2563348/1‑68 (MQ=255) ttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCAt > 1:2415964/1‑68 (MQ=255) ttACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCAt > 1:2252410/1‑68 (MQ=255) ccAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAgc > 1:3351888/1‑69 (MQ=255) ccAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAgc > 1:3315393/1‑69 (MQ=255) ccAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAgc > 1:1276040/1‑69 (MQ=255) aTGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAg < 1:1875347/52‑1 (MQ=255) tGCTGTAGATGTGTTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCa < 1:2499759/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCa < 1:2594890/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCa < 1:1429997/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCa < 1:863065/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCa < 1:531738/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:2312177/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:262711/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:875258/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:3024413/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:1278626/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:1882531/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:1878815/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:1285844/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:1798290/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:175995/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:1744129/69‑1 (MQ=255) tGCTGTAGATGTGTTTCGGACGACCATACACTTACGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:2426769/69‑1 (MQ=255) gCTGTAGATGTGTTTCGGTCGACCATACACTTCAGCTTTAACACCTACAGCTTTCATCTCAGCGCGCa < 1:1157505/68‑1 (MQ=255) gCTGTAGATGTGTTTCGGACGACCATAGACTTCCGCTTTAACGCCTTCa < 1:2582384/49‑1 (MQ=255) gtgtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:622342/59‑1 (MQ=255) gtgtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:965747/59‑1 (MQ=255) gtgtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:3369300/59‑1 (MQ=255) gtgtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:969798/59‑1 (MQ=255) gtgtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:2515459/59‑1 (MQ=255) gtgtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:2339363/59‑1 (MQ=255) gtgtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCa < 1:1625744/59‑1 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:1486866/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:3334062/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:163754/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:2025154/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:3396844/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:3423460/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:1224723/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:2155422/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAcaa > 1:942004/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAca > 1:1220239/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGAca > 1:3308541/1‑67 (MQ=255) gtTTCGGTCGACCATACACTTCAGCTTTAACACCTTCAGCTTTCAACTCAGCGCGCAGATGGCCGAcaa > 1:1750404/1‑67 (MQ=255) acgacCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCa < 1:1371645/58‑1 (MQ=255) cacTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAgtg > 1:1543971/1‑69 (MQ=255) cacTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAgtg > 1:923619/1‑69 (MQ=255) tCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTcg > 1:1205101/1‑69 (MQ=255) tCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTcg > 1:3196501/1‑69 (MQ=255) tCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTcg > 1:2560801/1‑69 (MQ=255) tCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTcg > 1:2114627/1‑69 (MQ=255) tCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTcg > 1:3053387/1‑69 (MQ=255) tttAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCgg > 1:1245528/1‑67 (MQ=255) tAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCga > 1:3274440/1‑69 (MQ=255) tAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCga > 1:2473459/1‑69 (MQ=255) tAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCga > 1:1307668/1‑69 (MQ=255) tAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCga > 1:798432/1‑69 (MQ=255) tAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCga > 1:2774766/1‑69 (MQ=255) tAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCga > 1:2800208/1‑69 (MQ=255) tAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCga > 1:473241/1‑69 (MQ=255) tAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCga > 1:300113/1‑69 (MQ=255) tAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCga > 1:1810400/1‑69 (MQ=255) tAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCga > 1:3186839/1‑69 (MQ=255) tAACACCTTCAGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCg > 1:3069598/1‑68 (MQ=255) aCGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCgat < 1:2170942/68‑2 (MQ=255) aCGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCgag < 1:1600824/68‑1 (MQ=255) aCGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCgag < 1:2061310/68‑1 (MQ=255) aCGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCgag < 1:2047790/68‑1 (MQ=255) aCGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCgag < 1:817783/68‑1 (MQ=255) aCGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCgag < 1:2116478/68‑1 (MQ=255) aCGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCgag < 1:945470/68‑1 (MQ=255) aCGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCgag < 1:1668544/68‑1 (MQ=255) | TCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAG > minE/1882341‑1882467 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |