| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,882,471 | C→G | 41.4% | R221R (CGG→CGC) | relA ← | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,882,471 | 0 | C | G | 41.4% | 45.5 / 85.6 | 87 | R221R (CGG→CGC) | relA | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Reads supporting (aligned to +/- strand): ref base C (37/14); new base G (30/6); total (67/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
CCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAA > minE/1882403‑1882534 | ccTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGc > 1:108994/1‑69 (MQ=255)ccTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGc < 1:1117741/69‑1 (MQ=255)ccTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGc < 1:2852946/69‑1 (MQ=255)ccTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGc < 1:2230276/69‑1 (MQ=255)ccTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGc > 1:1261153/1‑69 (MQ=255)ccTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCATCGATGTAGTGTTCGCGGTCGAGACGc > 1:1001572/1‑69 (MQ=255) aGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTc > 1:2498353/1‑69 (MQ=255) aGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTc > 1:993432/1‑69 (MQ=255) aGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTc > 1:813199/1‑69 (MQ=255) aGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTc > 1:784297/1‑69 (MQ=255) aGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTc > 1:3425598/1‑69 (MQ=255) aGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTc > 1:326354/1‑69 (MQ=255) aGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTc > 1:2982266/1‑69 (MQ=255) aGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTc > 1:2127774/1‑69 (MQ=255) aGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTc > 1:1826230/1‑69 (MQ=255) aGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTc > 1:1282195/1‑69 (MQ=255) aGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTc > 1:1094103/1‑69 (MQ=255) aGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTc > 1:1162218/1‑69 (MQ=255) aGCTTTCATCTCAGCGCGCAGATGGCCGACAAACTCTTCGATATAGTGTTCGAGGTCGAGACGGCGTTc > 1:1476047/1‑69 (MQ=255) tcAGCGCGCAGATGACCAACGAACTCTTGGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGcagcag > 1:408954/1‑68 (MQ=255) tcAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGCCGCCGTTc > 1:1434567/1‑59 (MQ=255) tcAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGcagcag > 1:2889483/1‑68 (MQ=255) tcAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGt > 1:2536300/1‑69 (MQ=255) tcAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGt > 1:3230208/1‑69 (MQ=255) tcAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGt > 1:2204763/1‑69 (MQ=255) tcAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGt > 1:3045225/1‑69 (MQ=255) tcAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGt > 1:2382615/1‑69 (MQ=255) tcAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGt > 1:2397043/1‑69 (MQ=255) tcAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGt > 1:2464144/1‑69 (MQ=255) tcAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGt > 1:97862/1‑69 (MQ=255) tcAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGt > 1:3003315/1‑69 (MQ=255) tcAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGt > 1:2648128/1‑69 (MQ=255) tcAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGt > 1:2789928/1‑69 (MQ=255) tcAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGt > 1:2917197/1‑69 (MQ=255) tcAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGt > 1:1468726/1‑69 (MQ=255) tcAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGt > 1:177649/1‑69 (MQ=255) tcAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGt > 1:1753290/1‑69 (MQ=255) tcAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGt > 1:1726956/1‑69 (MQ=255) tcAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGt > 1:1731405/1‑69 (MQ=255) gcgcAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGc < 1:462879/69‑1 (MQ=255) cgcAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGc < 1:931889/68‑1 (MQ=255) aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:3033302/1‑69 (MQ=255) aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:2918376/1‑69 (MQ=255) aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:1499234/1‑69 (MQ=255) aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:2685614/1‑69 (MQ=255) aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:40370/1‑69 (MQ=255) aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:2953200/1‑69 (MQ=255) aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:2527941/1‑69 (MQ=255) aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:1744934/1‑69 (MQ=255) aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:1960610/1‑69 (MQ=255) aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:1748761/1‑69 (MQ=255) aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:105451/1‑69 (MQ=255) aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:609138/1‑69 (MQ=255) aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:1953317/1‑69 (MQ=255) aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:3137745/1‑69 (MQ=255) aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:1958920/1‑69 (MQ=255) aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCg > 1:494796/1‑68 (MQ=255) aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGACGCGTTTGTATTCgg > 1:1684632/1‑69 (MQ=255) ctctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGt > 1:1026804/1‑69 (MQ=255) ctctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGt > 1:44130/1‑69 (MQ=255) ctctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGt > 1:2556375/1‑69 (MQ=255) ctctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGt > 1:1020897/1‑69 (MQ=255) ctctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGt > 1:3075952/1‑69 (MQ=255) ctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTtg < 1:2942334/69‑1 (MQ=255) ctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTtg < 1:572079/69‑1 (MQ=255) ctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTtg < 1:1587166/69‑1 (MQ=255) ctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTtg < 1:203926/69‑1 (MQ=255) ctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTtg < 1:1227256/69‑1 (MQ=255) ctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTtg < 1:2285505/69‑1 (MQ=255) ctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTtg < 1:257554/69‑1 (MQ=255) tatAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCGGtc < 1:1831722/60‑2 (MQ=255) tatAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCGGtc < 1:3365970/60‑2 (MQ=255) tatAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCGGtc < 1:1258621/60‑2 (MQ=255) tatAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCGGtc < 1:635111/60‑2 (MQ=255) tatAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCGGtc < 1:695325/60‑2 (MQ=255) tatAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATACGTTTGTATTCGGtc < 1:1335950/60‑2 (MQ=255) tAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAg > 1:95214/1‑68 (MQ=255) ttCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAAc > 1:587110/1‑68 (MQ=255) ttCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAAc > 1:1015467/1‑68 (MQ=255) ttCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAAc > 1:490955/1‑68 (MQ=255) ttCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAAc > 1:3058751/1‑68 (MQ=255) ttCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAAc > 1:303488/1‑68 (MQ=255) ttCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAAc > 1:2492542/1‑68 (MQ=255) ttCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAAc > 1:1232638/1‑68 (MQ=255) ttCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAAc > 1:2099099/1‑68 (MQ=255) cGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGt > 1:718776/1‑69 (MQ=255) cGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGt > 1:1149590/1‑69 (MQ=255) cGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGt > 1:2202785/1‑69 (MQ=255) gagaCGCCGTTCATGCAGCAGTTTGTCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTa < 1:3299596/69‑1 (MQ=255) gagaCGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTa < 1:727243/69‑1 (MQ=255) gagaCGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTa < 1:1889420/69‑1 (MQ=255) gaCGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTaa < 1:111208/68‑1 (MQ=255) aCGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTaa < 1:704680/67‑1 (MQ=255) | CCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAA > minE/1882403‑1882534 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |