| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,882,495 | T→G | 52.2% | R213R (CGA→CGC) | relA ← | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,882,495 | 0 | T | G | 52.2% | 15.5 / 92.7 | 86 | R213R (CGA→CGC) | relA | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Reads supporting (aligned to +/- strand): ref base T (28/13); new base G (17/27); total (45/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.95e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.74e-01 | |||||||||||
ACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGT > minE/1882440‑1882558 | aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:609138/1‑69 (MQ=255)aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:1744934/1‑69 (MQ=255)aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:1748761/1‑69 (MQ=255)aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:1953317/1‑69 (MQ=255)aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:1958920/1‑69 (MQ=255)aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:1499234/1‑69 (MQ=255)aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:1960610/1‑69 (MQ=255)aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:2527941/1‑69 (MQ=255)aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:2685614/1‑69 (MQ=255)aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:2918376/1‑69 (MQ=255)aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:2953200/1‑69 (MQ=255)aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:3033302/1‑69 (MQ=255)aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:3137745/1‑69 (MQ=255)aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:105451/1‑69 (MQ=255)aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCgg > 1:40370/1‑69 (MQ=255)aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCg > 1:494796/1‑68 (MQ=255)aCTCTTCGATATAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGACGCGTTTGTATTCgg > 1:1684632/1‑69 (MQ=255) ctctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGt > 1:1020897/1‑69 (MQ=255) ctctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGt > 1:2556375/1‑69 (MQ=255) ctctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGt > 1:3075952/1‑69 (MQ=255) ctctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGt > 1:44130/1‑69 (MQ=255) ctctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGt > 1:1026804/1‑69 (MQ=255) ctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTtg < 1:572079/69‑1 (MQ=255) ctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTtg < 1:1227256/69‑1 (MQ=255) ctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTtg < 1:2942334/69‑1 (MQ=255) ctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTtg < 1:1587166/69‑1 (MQ=255) ctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTtg < 1:257554/69‑1 (MQ=255) ctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTtg < 1:203926/69‑1 (MQ=255) ctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTtg < 1:2285505/69‑1 (MQ=255) tatAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCGGtc < 1:3365970/60‑2 (MQ=255) tatAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCGGtc < 1:1258621/60‑2 (MQ=255) tatAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCGGtc < 1:635111/60‑2 (MQ=255) tatAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCGGtc < 1:695325/60‑2 (MQ=255) tatAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATGCGTTTGTATTCGGtc < 1:1831722/60‑2 (MQ=255) tatAGTGTTCGCGGTCGAGACGGCGTTCATGCAGCAGTTTTGCGATACGTTTGTATTCGGtc < 1:1335950/60‑2 (MQ=255) tAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAg > 1:95214/1‑68 (MQ=255) ttCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAAc > 1:2099099/1‑68 (MQ=255) ttCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAAc > 1:587110/1‑68 (MQ=255) ttCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAAc > 1:1015467/1‑68 (MQ=255) ttCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAAc > 1:2492542/1‑68 (MQ=255) ttCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAAc > 1:3058751/1‑68 (MQ=255) ttCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAAc > 1:303488/1‑68 (MQ=255) ttCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAAc > 1:490955/1‑68 (MQ=255) ttCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAAc > 1:1232638/1‑68 (MQ=255) cGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGt > 1:2202785/1‑69 (MQ=255) cGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGt > 1:1149590/1‑69 (MQ=255) cGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGt > 1:718776/1‑69 (MQ=255) gagaCGCCGTTCATGCAGCAGTTTGTCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTa < 1:3299596/69‑1 (MQ=255) gagaCGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTa < 1:1889420/69‑1 (MQ=255) gagaCGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTa < 1:727243/69‑1 (MQ=255) gaCGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTaa < 1:111208/68‑1 (MQ=255) aCGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTaa < 1:704680/67‑1 (MQ=255) ttCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAg < 1:130944/69‑1 (MQ=255) cATGCAGCAGTTTTGCGATGCGTTTGTATTCGGtc < 1:3133216/35‑2 (MQ=21) cATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTtgga > 1:2985487/1‑38 (MQ=255) cagcagTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCa > 1:3377752/1‑69 (MQ=255) cagcagTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCa > 1:2261358/1‑69 (MQ=255) cagcagTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCa > 1:2380264/1‑69 (MQ=255) cagcagTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCa > 1:1214323/1‑69 (MQ=255) cagcagTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCa > 1:1388132/1‑69 (MQ=255) cagcagTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCa > 1:1940723/1‑69 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:82555/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:969835/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:608927/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:676439/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:932736/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:814666/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:2676234/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:1158111/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:1582224/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:1737417/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:1752842/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:1816819/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:2043337/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:41924/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:2720051/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:2906138/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:3250587/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:3325428/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:3357433/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:3378177/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:3425352/69‑1 (MQ=255) gCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGt > 1:2763577/1‑69 (MQ=255) gCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGt > 1:2573965/1‑69 (MQ=255) gCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGt > 1:1259529/1‑69 (MQ=255) gCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTACAGTTCCCATTTCAGTTg > 1:2082731/1‑68 (MQ=255) | ACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGT > minE/1882440‑1882558 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |