| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,882,543 | T→C | 35.8% | E197E (GAA→GAG) | relA ← | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,882,543 | 0 | T | C | 35.8% | 66.4 / 65.0 | 106 | E197E (GAA→GAG) | relA | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Reads supporting (aligned to +/- strand): ref base T (40/28); new base C (17/21); total (57/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 106 total aligned reads displayed.
CAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACT > minE/1882480‑1882604 | cagcagTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCa > 1:2261358/1‑69 (MQ=255)cagcagTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCa > 1:1214323/1‑69 (MQ=255)cagcagTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCa > 1:1940723/1‑69 (MQ=255)cagcagTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCa > 1:1388132/1‑69 (MQ=255)cagcagTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCa > 1:2380264/1‑69 (MQ=255)cagcagTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCa > 1:3377752/1‑69 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:676439/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:41924/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:2043337/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:3425352/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:608927/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:3378177/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:1816819/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:1752842/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:1737417/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:3357433/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:814666/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:82555/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:3325428/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:3250587/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:2906138/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:1158111/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:2720051/69‑1 (MQ=255) agTTTTGCGATGCGTTTGTATTCGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTc < 1:932736/69‑1 (MQ=255) gCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGt > 1:2573965/1‑69 (MQ=255) gCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGt > 1:1259529/1‑69 (MQ=255) gCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGt > 1:2763577/1‑69 (MQ=255) gCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTACAGTTCCCATTTCAGTTg > 1:2082731/1‑68 (MQ=255) cGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGAtt > 1:630237/1‑69 (MQ=255) cGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGAt > 1:1879769/1‑68 (MQ=255) ttGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTcc < 1:1009684/68‑1 (MQ=255) ttGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTcc < 1:902205/68‑1 (MQ=255) tGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCa < 1:368475/54‑1 (MQ=255) tGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCa < 1:2987386/54‑1 (MQ=255) tGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCa < 1:2624758/54‑1 (MQ=255) tGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCa < 1:866920/54‑1 (MQ=255) tGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCa < 1:1288670/54‑1 (MQ=255) tGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCa < 1:3304265/54‑1 (MQ=255) tGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCa < 1:1329220/54‑1 (MQ=255) tGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCa < 1:1646705/54‑1 (MQ=255) tGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCa < 1:1938936/54‑1 (MQ=255) tGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCa < 1:1942913/54‑1 (MQ=255) tGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCa < 1:3418686/54‑1 (MQ=255) tGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCa < 1:2197147/54‑1 (MQ=255) tGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCa < 1:34957/54‑1 (MQ=255) tGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCa < 1:2014901/54‑1 (MQ=255) cGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTCAGTTgcc > 1:1533228/1‑52 (MQ=255) cGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTCAGTTGCCCAATCCCAAGACgg > 1:2009878/1‑68 (MQ=255) cGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTCAGTTGCCCAATCCCAAGACGGt > 1:68216/1‑69 (MQ=255) cGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTCAGTTGCCCAATCCCAAGACGGt > 1:3150077/1‑69 (MQ=255) cGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTCAGTTGCCCAATCCCAAGACGGt > 1:3080652/1‑69 (MQ=255) cGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTCAGTTGCCCAATCCCAAGACGGt > 1:902143/1‑69 (MQ=255) cGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTCAGTTGCCCAATCCCAAGACGGt > 1:2765971/1‑69 (MQ=255) cGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTCAGTTGCCCAATCCCAAGACGGt > 1:101507/1‑69 (MQ=255) cGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTCAGTTGCCCAATCCCAAGACGGt > 1:1057763/1‑69 (MQ=255) cGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTCAGTTGCCCAATCCCAAGACGGt > 1:13652/1‑69 (MQ=255) cGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTCAGTTGCCCAATCCCAAGACGGt > 1:2081028/1‑69 (MQ=255) cGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTCAGTTGCCCAATCCCAAGACGGt > 1:2250463/1‑69 (MQ=255) cGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTCAGTTGCCCAATCCCAAGACGGt > 1:2384745/1‑69 (MQ=255) cGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTCAGTTGCCCAATCCCAAGACGGt > 1:2391988/1‑69 (MQ=255) cGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTCAGTTGCCCAATCCCAAGACGGt > 1:2497943/1‑69 (MQ=255) cGGTCGGGTGCAGATAACGGAAGCAGTAATCTTCCAGCTCCCATTTCAGTTGCCCAATCCCAAGACGGt > 1:2582755/1‑69 (MQ=255) ttGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTa < 1:2800472/68‑1 (MQ=255) gaGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCgg < 1:3414339/69‑1 (MQ=255) aGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCa < 1:1984371/37‑1 (MQ=255) aCGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTg > 1:2703474/1‑65 (MQ=255) aCGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTg > 1:751398/1‑65 (MQ=255) aCGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTg > 1:2955071/1‑65 (MQ=255) aCGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTg > 1:2985902/1‑65 (MQ=255) aCGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTa < 1:388952/69‑1 (MQ=255) aCGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTa < 1:3011918/69‑1 (MQ=255) gTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGt < 1:2997409/69‑1 (MQ=255) aaTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGtt > 1:2347577/1‑43 (MQ=255) aaTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTac > 1:2976189/1‑69 (MQ=255) aaTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTac > 1:2597128/1‑69 (MQ=255) aaTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTac > 1:1124175/1‑69 (MQ=255) aaTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTac > 1:344504/1‑69 (MQ=255) aaTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTac > 1:2125650/1‑69 (MQ=255) aaTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTac > 1:1264588/1‑69 (MQ=255) aaTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTa > 1:1699627/1‑68 (MQ=255) aTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCTTAGa > 1:1179977/1‑59 (MQ=255) aTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGa > 1:2380872/1‑59 (MQ=255) aTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGa > 1:89679/1‑59 (MQ=255) aTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGa > 1:1457291/1‑59 (MQ=255) aTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGa < 1:377140/59‑1 (MQ=255) aTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTaca > 1:3165560/1‑69 (MQ=255) aTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTaca > 1:2906250/1‑69 (MQ=255) aTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTaca > 1:2675768/1‑69 (MQ=255) aTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTaca > 1:1422265/1‑69 (MQ=255) aTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTaca > 1:909789/1‑69 (MQ=255) aTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTaca > 1:3305699/1‑69 (MQ=255) aTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTac > 1:2497726/1‑68 (MQ=255) cTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACgg > 1:2094695/1‑38 (MQ=255) cTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTacac < 1:634684/68‑1 (MQ=255) cTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTacac < 1:1520306/68‑1 (MQ=255) cTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTacac < 1:2683391/68‑1 (MQ=255) cTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTacac < 1:879373/68‑1 (MQ=255) cTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACAct > 1:548660/1‑69 (MQ=255) cTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACAct > 1:403243/1‑69 (MQ=255) cTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACAct > 1:2211859/1‑69 (MQ=255) | CAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACT > minE/1882480‑1882604 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |