| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,882,657 | A→G | 52.2% | H159H (CAT→CAC) | relA ← | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,882,657 | 0 | A | G | 52.2% | 6.9 / 81.5 | 94 | H159H (CAT→CAC) | relA | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Reads supporting (aligned to +/- strand): ref base A (16/29); new base G (33/16); total (49/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.59e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.37e-01 | |||||||||||
GTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCAT > minE/1882594‑1882710 | gTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGc < 1:2821369/69‑1 (MQ=255)gTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGc < 1:1144364/69‑1 (MQ=255)gTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGc < 1:270179/69‑1 (MQ=255)gTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGc < 1:585271/69‑1 (MQ=255)gTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGc < 1:416966/69‑1 (MQ=255)gTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGc < 1:1420889/69‑1 (MQ=255)gTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGc < 1:316417/69‑1 (MQ=255)gTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGc < 1:1539885/69‑1 (MQ=255) ttGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGc < 1:662094/68‑1 (MQ=255) ttGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGc < 1:2961875/68‑1 (MQ=255) tGGTGCACTCTTTTGCCGCCAGTACGCGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCaa > 1:2117924/1‑69 (MQ=255) tGGTGCACTCTTTTGCCGCCAGTACGCGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCaa > 1:256301/1‑69 (MQ=255) tGGTGCACTCTTTTGCCGCCAGTACGCGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCaa > 1:2403728/1‑69 (MQ=255) tGGTGCACTCTTTTGCCGCCAGTACGCGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCaa > 1:2395103/1‑69 (MQ=255) tGGTGCACTCTTTTGCCGCCAGTACGCGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCaa > 1:2994645/1‑69 (MQ=255) tGGTGCACTCTTTTGCCGCCAGTACGCGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCaa > 1:2075896/1‑69 (MQ=255) tGGTGCACTCTTTTGCCGCCAGTACGCGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCaa > 1:2071847/1‑69 (MQ=255) tGGTGCACTCTTTTGCCGCCAGTACGCGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCaa > 1:2957808/1‑69 (MQ=255) tGGTGCACTCTTTTGCCGCCAGTACGCGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCaa > 1:1047297/1‑69 (MQ=255) tGGTGCACTCTTTTGCCGCCAGTACGCGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCaa > 1:3045006/1‑69 (MQ=255) tGGTGCACTCTTTTGCCGCCAGTACGCGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCaa > 1:3120332/1‑69 (MQ=255) tGGTGCACTCTTTTGCCGCCAGTACGCGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCaa > 1:3413378/1‑69 (MQ=255) tGGTGCACTCTTTTGCCGCCAGTACGCGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCaa > 1:823207/1‑69 (MQ=255) tGGTGCACTCTTTTGCCGCCAGTACGCGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCaa > 1:865391/1‑69 (MQ=255) tGGTGCACTCTTTTGCCGCCAGTACGCGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCaa > 1:1967516/1‑69 (MQ=255) tGGTGCACTCTTTTGCCGCCAGTACGCGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCaa > 1:1639414/1‑69 (MQ=255) tGGTGCACTCTTTTGCCGCCAGTACGCGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCaa > 1:1692403/1‑69 (MQ=255) tGGTGCACTCTTTTGCCGCCAGTACGCGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCaa > 1:1499181/1‑69 (MQ=255) tGGTGCACTCTTTTGCCGCCAGTACGCGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCaa > 1:1831716/1‑69 (MQ=255) tGGTGCACTCTTTTGCCGCCAGTACGCGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCaa > 1:1408440/1‑69 (MQ=255) ggTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCaa < 1:2330417/68‑1 (MQ=255) ggTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCaa < 1:2827070/68‑1 (MQ=255) ggTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCaa < 1:2697463/68‑1 (MQ=255) ggTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCaa < 1:3032996/68‑1 (MQ=255) ggTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCaa < 1:2177963/68‑1 (MQ=255) ggTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCaa < 1:2145369/68‑1 (MQ=255) cacTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGc < 1:616945/69‑1 (MQ=255) cacTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGc < 1:2645763/69‑1 (MQ=255) cacTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGc < 1:2460150/69‑1 (MQ=255) cacTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGc < 1:2998861/69‑1 (MQ=255) cacTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGc < 1:3304077/69‑1 (MQ=255) cacTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGc < 1:900311/69‑1 (MQ=255) cacTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGc < 1:1572796/69‑1 (MQ=255) ctctTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATTAGCAATACGCTc > 1:2682289/1‑69 (MQ=255) ctctTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCt > 1:2854429/1‑68 (MQ=255) ctctTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTc > 1:1929223/1‑69 (MQ=255) ctctTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTc > 1:2122239/1‑69 (MQ=255) ctctTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTc > 1:2507911/1‑69 (MQ=255) cacGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTtg > 1:2267544/1‑62 (MQ=255) cacGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTtg > 1:791698/1‑62 (MQ=255) cacGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTtg > 1:24972/1‑62 (MQ=255) cacGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTtg < 1:1539490/62‑1 (MQ=255) acGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTtg < 1:501018/61‑1 (MQ=255) ttCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCa < 1:1707587/69‑1 (MQ=255) ttCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCa < 1:1037158/69‑1 (MQ=255) ttCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCa < 1:467521/69‑1 (MQ=255) cATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCa > 1:3059651/1‑38 (MQ=255) cATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCAATACGCTcg > 1:1172018/1‑46 (MQ=255) cATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCAATACGCTcc > 1:2970368/1‑47 (MQ=255) cATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCAATACGCTcc > 1:998618/1‑47 (MQ=255) cATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCAATACGCTcc > 1:357341/1‑47 (MQ=255) cATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCAATACGCTcc > 1:488277/1‑47 (MQ=255) cATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCAATACGCTcc > 1:1576738/1‑47 (MQ=255) cATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCAATACGCTcc > 1:1734953/1‑47 (MQ=255) cATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCAATACGCTcc > 1:1935219/1‑47 (MQ=255) cATCTTCCGGCGCATCTTTTACTTCGCGCAGGTGAGCAATACGCTc > 1:2346894/1‑46 (MQ=255) cATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCaa < 1:2593842/39‑1 (MQ=255) ccGGCGCATCTTTTACTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAat > 1:2856987/1‑69 (MQ=255) ccGGCGCATCTTTTACTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAat > 1:1454363/1‑69 (MQ=255) ccGGCGCATCTTTTACTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAat > 1:1466667/1‑69 (MQ=255) cTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGAc > 1:2630309/1‑67 (MQ=255) cTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGAc > 1:2878482/1‑67 (MQ=255) cTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGAc > 1:1983126/1‑67 (MQ=255) cTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGAc > 1:1987240/1‑67 (MQ=255) cTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGAc > 1:2200071/1‑67 (MQ=255) ttttACTTCGCGCAGATGAGCAATACGCTCCGCCa > 1:1967615/1‑35 (MQ=255) ttttACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCAt > 1:1287920/1‑69 (MQ=255) ttttACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCAt > 1:177736/1‑69 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:2315389/65‑1 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:3359949/65‑1 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:3102358/65‑1 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:2207241/65‑1 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:3090946/65‑1 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:2196941/65‑1 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:2178268/65‑1 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:2132390/65‑1 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:1274307/65‑1 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:704763/65‑1 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:1521358/65‑1 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:2022466/65‑1 (MQ=255) aCTTCGCGCACGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:3022766/65‑1 (MQ=255) aCTTCGCCCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:2078308/65‑1 (MQ=255) cTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:3357073/64‑1 (MQ=255) gcAGGTGAGCAATACGCTCCGCCTGTTTGATGACCACGCACCGAAAATCATCGACCAt < 1:2365965/58‑1 (MQ=255) | GTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCAT > minE/1882594‑1882710 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |