| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,882,687 | T→C | 31.6% | V149V (GTA→GTG) | relA ← | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,882,687 | 0 | T | C | 31.6% | 47.9 / 28.7 | 60 | V149V (GTA→GTG) | relA | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Reads supporting (aligned to +/- strand): ref base T (22/19); new base C (3/16); total (25/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.02e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGA > minE/1882624‑1882752 | ttCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCa < 1:1037158/69‑1 (MQ=255)ttCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCa < 1:1707587/69‑1 (MQ=255)ttCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCa < 1:467521/69‑1 (MQ=255) ccGGCGCATCTTTTACTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAat > 1:2856987/1‑69 (MQ=255) ccGGCGCATCTTTTACTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAat > 1:1466667/1‑69 (MQ=255) ccGGCGCATCTTTTACTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAat > 1:1454363/1‑69 (MQ=255) cTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGAc > 1:2878482/1‑67 (MQ=255) cTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGAc > 1:1987240/1‑67 (MQ=255) cTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGAc > 1:2630309/1‑67 (MQ=255) cTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGAc > 1:1983126/1‑67 (MQ=255) cTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGAc > 1:2200071/1‑67 (MQ=255) ttttACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCAt > 1:1287920/1‑69 (MQ=255) ttttACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCAt > 1:177736/1‑69 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:2132390/65‑1 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:3359949/65‑1 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:2178268/65‑1 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:2196941/65‑1 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:2207241/65‑1 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:2315389/65‑1 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:704763/65‑1 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:1274307/65‑1 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:3090946/65‑1 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:3102358/65‑1 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:2022466/65‑1 (MQ=255) aCTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:1521358/65‑1 (MQ=255) aCTTCGCGCACGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:3022766/65‑1 (MQ=255) aCTTCGCCCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:2078308/65‑1 (MQ=255) cTTCGCGCAGGTGAGCAATACGCTCCGCCAGTTTGATGACCACGCAGCGAAAATCATCGACCAt < 1:3357073/64‑1 (MQ=255) gcAGGTGAGCAATACGCTCCGCCTGTTTGATGACCACGCACCGAAAATCATCGACCAt < 1:2365965/58‑1 (MQ=255) gAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCgg > 1:539210/1‑68 (MQ=255) gAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCgg > 1:459008/1‑68 (MQ=255) gAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCgg > 1:1734711/1‑68 (MQ=255) gAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCgg > 1:2502835/1‑68 (MQ=255) tACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGt > 1:2761929/1‑69 (MQ=255) cGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGaa > 1:1580214/1‑64 (MQ=255) cGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGtt > 1:1523546/1‑68 (MQ=255) cGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTa > 1:328165/1‑69 (MQ=255) cGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTa < 1:3109962/69‑1 (MQ=255) cGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTa > 1:3327121/1‑69 (MQ=255) cGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTa < 1:623258/69‑1 (MQ=255) cGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTa > 1:867540/1‑69 (MQ=255) cGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTa < 1:882634/69‑1 (MQ=255) cGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTa < 1:283680/69‑1 (MQ=255) cGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTa > 1:1682551/1‑69 (MQ=255) cGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTa < 1:1980739/69‑1 (MQ=255) cGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTa > 1:1977846/1‑69 (MQ=255) cGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTa > 1:2468021/1‑69 (MQ=255) gaCTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCggaggt < 1:2113116/69‑2 (MQ=255) gaCTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCggagga < 1:1427257/69‑1 (MQ=255) gaCTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCggagga < 1:2399198/69‑1 (MQ=255) aCTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATc < 1:2395539/53‑1 (MQ=255) aCTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATc < 1:1629241/53‑1 (MQ=255) aCTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATc < 1:35635/53‑1 (MQ=255) aCTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATc < 1:1784200/53‑1 (MQ=255) aCTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATc < 1:135080/53‑1 (MQ=255) aCTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATc < 1:3238588/53‑1 (MQ=255) aCTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATc < 1:2791272/53‑1 (MQ=255) aCTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATc < 1:2109558/53‑1 (MQ=255) aCTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCggagga > 1:316913/1‑68 (MQ=255) aCTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCggagga > 1:1283263/1‑68 (MQ=255) | TTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGA > minE/1882624‑1882752 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |