| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,883,630 | G→C | 41.1% | Q285E (CAA→GAA) | rumA ← | 23S rRNA (uracil‑5)‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,883,630 | 0 | G | C | 41.1% | 36.8 / 72.6 | 73 | Q285E (CAA→GAA) | rumA | 23S rRNA (uracil‑5)‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (20/23); new base C (17/13); total (37/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01 | |||||||||||
GTCGCCAATGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGTTCACACCCGCATTGACCTGA > minE/1883566‑1883691 | gtCGCCAATGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGca < 1:1623564/67‑1 (MQ=255) aaTGGCAGTGTAAAGTTGCCCATACCTCAGAACAGATCCAGTACGCGATCTTCAGGTTCCACATCCa < 1:10626/67‑1 (MQ=255) aaTGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTCCACATCCa < 1:1009206/67‑1 (MQ=255) aaTGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTCCACATCCa < 1:3029565/67‑1 (MQ=255) aaTGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTCCACATCCa < 1:371509/67‑1 (MQ=255) aaTGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTCCACATCCa < 1:2984740/67‑1 (MQ=255) aaTGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTCCACATCCa < 1:451456/67‑1 (MQ=255) aaTGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTCCACATCCa < 1:535502/67‑1 (MQ=255) aaTGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTCCACATCCa < 1:1359582/67‑1 (MQ=255) aaTGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTCCACATCCa < 1:2548265/67‑1 (MQ=255) aaTGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTCCACATCCa < 1:2383769/67‑1 (MQ=255) aaTGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTCCACATCCa < 1:151202/67‑1 (MQ=255) aaTGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTCCACATCCa < 1:2309066/67‑1 (MQ=255) ggCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCAt > 1:680898/1‑69 (MQ=255) ggCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCAt > 1:3258151/1‑69 (MQ=255) ggCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCAt > 1:2114615/1‑69 (MQ=255) ggCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCAt > 1:2680706/1‑69 (MQ=255) ggCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCAt > 1:1280748/1‑69 (MQ=255) ggCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCAt > 1:902324/1‑69 (MQ=255) aGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTcc < 1:1835266/69‑1 (MQ=255) aGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTcc < 1:1965688/69‑1 (MQ=255) aGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTcc < 1:1108737/69‑1 (MQ=255) cATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCAcgcg > 1:1715452/1‑65 (MQ=255) cATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTAc > 1:796875/1‑69 (MQ=255) cATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTAc > 1:1254836/1‑69 (MQ=255) cATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTAc > 1:1218070/1‑69 (MQ=255) cATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTAc > 1:2970540/1‑69 (MQ=255) cATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTAc > 1:3433805/1‑69 (MQ=255) cATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTAc > 1:2171088/1‑69 (MQ=255) cATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTAc > 1:330057/1‑69 (MQ=255) cATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTAc > 1:3187586/1‑69 (MQ=255) cATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTAc > 1:1882575/1‑69 (MQ=255) cATACCGCAGAACAGATCCAGAACGCGATCTTCAGCTTGCACAt > 1:1171626/1‑44 (MQ=38) cGCAGAACAGATCCAGTACGCGATCTTCAGGTTCCACATCCa < 1:2035521/42‑1 (MQ=255) cAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGtt < 1:2226747/69‑1 (MQ=255) cAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGtt < 1:3208038/69‑1 (MQ=255) cAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGtt < 1:328782/69‑1 (MQ=255) cAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGtt < 1:2602291/69‑1 (MQ=255) cAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGtt < 1:1827694/69‑1 (MQ=255) cAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGtt < 1:268530/69‑1 (MQ=255) aGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGtt < 1:1804816/68‑1 (MQ=255) aGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGtt < 1:2695644/68‑1 (MQ=255) aGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGtt < 1:2826666/68‑1 (MQ=255) tCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGTTcaca > 1:2602469/1‑69 (MQ=255) tCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGTTcaca > 1:1624337/1‑69 (MQ=255) gTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGTTCACACCCg < 1:2121798/69‑1 (MQ=255) gTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGTTCACACCCg < 1:2042004/69‑1 (MQ=255) cgcgATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGtt < 1:300710/58‑1 (MQ=255) cgATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCTCTACCATTTTTTGGTTCACACCCGCATTg < 1:2519852/69‑1 (MQ=255) cgATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCTCTACCATTTTTTGGTTCACACCCGCATTg < 1:2515780/69‑1 (MQ=255) cgATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGTTCACACCCGCATTg < 1:1506049/69‑1 (MQ=255) cgATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGTTCACACCCGCATTg < 1:2263245/69‑1 (MQ=255) cgATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGTTCACACCCGCATTg < 1:3124318/69‑1 (MQ=255) cgATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGTTCACACCCGCATTg < 1:2072153/69‑1 (MQ=255) tCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGtt < 1:1574612/53‑1 (MQ=255) cTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGTTCACACCCGCATTGAcc > 1:2771824/1‑68 (MQ=255) cAGGTTCCACATCCAGCCATTCCAACGCACGCGCTAcc > 1:1133627/1‑38 (MQ=255) cAGGTTCCACATCCAGCCATTCCAACGCACGCGCTACCATTTTCTGGTTCACACCCGCATTGACCTg > 1:662308/1‑67 (MQ=255) cAGGTTCCACATCCAGCCATTCCAACGCACGCGCTACCATTTTCTGGTTCACACCCGCATTGACCTg > 1:1031326/1‑67 (MQ=255) cAGGTTCCACATCCAGCCATTCCAACGCACGCGCTACCATTTTCTGGTTCACACCCGCATTGACCTg > 1:3152075/1‑67 (MQ=255) cAGGTTCCACATCCAGCCATTCCAACGCACGCGCTACCATTTTCTGGTTCACACCCGCATTGACCTg > 1:2880100/1‑67 (MQ=255) cAGGTTCCACATCCAGCCATTCCAACGCACGCGCTACCATTTTCTGGTTCACACCCGCATTGACCTg > 1:2758002/1‑67 (MQ=255) cAGGTTCCACATCCAGCCATTCCAACGCACGCGCTACCATTTTCTGGTTCACACCCGCATTGACCTGa > 1:3220960/1‑68 (MQ=255) cAGGTTCCACATCCAGCCATTCCAACGCACGCGCTACCATTTTCTGGTTCACACCCGCATTGACCTGa > 1:3212404/1‑68 (MQ=255) cAGGTTCCACATCCAGCCATTCCAACGCACGCGCTACCATTTTCTGGTTCACACCCGCATTGACCTGa > 1:3079580/1‑68 (MQ=255) cAGGTTCCACATCCAGCCATTCCAACGCACGCGCTACCATTTTCTGGTTCACACCCGCATTGACCTGa > 1:3434285/1‑68 (MQ=255) cAGGTTCCACATCCAGCCATTCCAACGCACGCGCTACCATTTTCTGGTTCACACCCGCATTGACCTGa > 1:3008571/1‑68 (MQ=255) cAGGTTCCACATCCAGCCATTCCAACGCACGCGCTACCATTTTCTGGTTCACACCCGCATTGACCTGa > 1:2742140/1‑68 (MQ=255) cAGGTTCCACATCCAGCCATTCCAACGCACGCGCTACCATTTTCTGGTTCACACCCGCATTGACCTGa > 1:1720517/1‑68 (MQ=255) cAGGTTCCACATCCAGCCATTCCAACGCACGCGCTACCATTTTCTGGTTCACACCCGCATTGACCTGa > 1:697053/1‑68 (MQ=255) cAGGTTCCACATCCAGCCATTCCAACGCACGCGCTACCATTTTCTGGTTCACACCCGCATTGACCTGa > 1:1640269/1‑68 (MQ=255) cAGGTTCCACATCCAGCCATTCCAACGCACGCGCTACCATTTTCTGGTTCACACCCGCATTGACCTGa > 1:1424200/1‑68 (MQ=255) cAGGTTCCACATCCAGCCATTCCAACGCACGCGCTACCATTTTCTGGTTCACACCCGCATTGACCTGa > 1:908470/1‑68 (MQ=255) gCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGtt < 1:3378823/43‑1 (MQ=255) | GTCGCCAATGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGTTCACACCCGCATTGACCTGA > minE/1883566‑1883691 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |