| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,884,080 | G→A | 11.9% | L135L (CTG→TTG) | rumA ← | 23S rRNA (uracil‑5)‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,884,080 | 0 | G | A | 11.9% | 124.9 / 4.2 | 66 | L135L (CTG→TTG) | rumA | 23S rRNA (uracil‑5)‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (32/26); new base A (4/4); total (36/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
GACAATGTCACTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCGTGT > minE/1884012‑1884144 | gACAATGTCACTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCAg > 1:3284550/1‑69 (MQ=255)gACAATGTCACTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCAg > 1:3280897/1‑69 (MQ=255)gACAATGTCACTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCAg > 1:1585246/1‑69 (MQ=255)gACAATGTCACTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCAg > 1:3223843/1‑69 (MQ=255) tGTCACTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCAGACTTa < 1:2066111/69‑1 (MQ=255) tGTCACTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCAGACTTa < 1:2064839/69‑1 (MQ=255) tGTCACTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCAGACTTa < 1:2907602/69‑1 (MQ=255) tGTCACTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCAGACTTa < 1:400329/69‑1 (MQ=255) gTCACTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCAGACTTa < 1:1469654/68‑1 (MQ=255) aCTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCAAACTTAAAcg > 1:465896/1‑69 (MQ=255) aCTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCAAACTTAAAcg > 1:1345723/1‑69 (MQ=255) aCTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCAAACTTAAAcg > 1:1194151/1‑69 (MQ=255) aCTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCAAACTTAAAcg > 1:3029055/1‑69 (MQ=255) gAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCAGACTTAAAcgcgc < 1:1630986/68‑1 (MQ=255) gCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCAGACTTAAAcgcgcgcg > 1:2510535/1‑69 (MQ=255) aaCCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCAAACTTAAACGCGCGCGACGGCGATAGccc < 1:37409/69‑1 (MQ=255) aaCCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCAAACTTAAACGCGCGCGACGGCGATAGccc < 1:3237394/69‑1 (MQ=255) aaCCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCAAACTTAAACGCGCGCGACGGCGATAGccc < 1:1319443/69‑1 (MQ=255) aaCCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCAAACTTAAACGCGCGCGACGGCGATAGccc < 1:2935318/69‑1 (MQ=255) ccATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAg > 1:1762890/1‑68 (MQ=255) gTTGCTGTGTTTTCGGTAAGTAGTTCAGACTTAAACGCGCGCGAc > 1:2511953/1‑45 (MQ=255) gTTGCTGTGTTTTCGGTAAGTAGTTCAGACTTAAACGCGCGCGAc > 1:964399/1‑45 (MQ=255) gTTGCTGTGTTTTCGGTAAGTAGTTCAGACTTAAACGCGCGCGAc > 1:3163714/1‑45 (MQ=255) gTTGCTGTGTTTTCGGTAAGTAGTTCAGACTTAAACGCGCGCGAc > 1:242017/1‑45 (MQ=255) gTTGCTGTGTTTTCGGTAAGTAGTTCAGACTTAAACGCGCGCGAc > 1:2216860/1‑45 (MQ=255) gTTGCTGTGTTTTCGGTAAGTAGTTCAGACTTAAACGCGCGCGAc > 1:87118/1‑45 (MQ=255) gTTGCTGTGTTTTCGGTAAGTAGTTCAGACTTAAACGCGCGCGAc > 1:3301880/1‑45 (MQ=255) ttGCTGTGTTTTCGGTAAGTAGTTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCgg > 1:3222684/1‑68 (MQ=255) ttGCTGTGTTTTCGGTAAGTAGTTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGc > 1:252022/1‑69 (MQ=255) ttGCTGTGTTTTCGGTAAGTAGTTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGc > 1:2995935/1‑69 (MQ=255) ttGCTGTGTTTTCGGTAAGTAGTTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGc > 1:2726562/1‑69 (MQ=255) ttGCTGTGTTTTCGGTAAGTAGTTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGc > 1:1004034/1‑69 (MQ=255) ttGCTGTGTTTTCGGTAAGTAGTTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGc > 1:506364/1‑69 (MQ=255) ttGCTGTGTTTTCGGTAAGTAGTTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGc > 1:1812769/1‑69 (MQ=255) ttGCTGTGTTTTCGGTAAGTAGTTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGc > 1:2270185/1‑69 (MQ=255) ttGCTGTGTTTTCGGTAAGTAGTTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGc > 1:3368932/1‑69 (MQ=255) ttGCTGTGTTTTCGGTAAGTAGTTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGc > 1:3248570/1‑69 (MQ=255) ttGCTGAGTTTTCGGTAAGTAGTTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGc > 1:866/1‑69 (MQ=255) tgtgTTTTCGGTAAGTAGTTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGAt < 1:710405/68‑1 (MQ=255) ttttCGGTAAGTAGTTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCAc < 1:1443729/67‑1 (MQ=255) ttttCGGTAAGTAGTTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCAc < 1:228525/67‑1 (MQ=255) tCGGTAAGTAGTTCAGACTTAAACGCGCGCGACGGc < 1:358465/36‑1 (MQ=255) tCGGTAAGTAGTTCAGACTTAAACGCGCGCGACGGc < 1:807956/36‑1 (MQ=255) tCGGTAAGTAGTTCAGACTTAAACGCGCGCGACGGc < 1:2685822/36‑1 (MQ=255) tCGGTAAGTAGTTCAGACTTAAACGCGCGCGACGGc < 1:2398776/36‑1 (MQ=255) tCGGTAAGTAGTTCAGACTTAAACGCGCGCGACGGc < 1:1826792/36‑1 (MQ=255) agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACTTCgt < 1:2940086/69‑1 (MQ=255) agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACTTCgt < 1:2763544/69‑1 (MQ=255) agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACTTCgt < 1:2736841/69‑1 (MQ=255) agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACTTCgt < 1:2214374/69‑1 (MQ=255) agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACTTCgt < 1:1068538/69‑1 (MQ=255) agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACTTCgt < 1:748581/69‑1 (MQ=255) agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACTTCgt < 1:1440012/69‑1 (MQ=255) agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACTTCgt < 1:3340140/69‑1 (MQ=255) agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACTTCgt < 1:1916190/69‑1 (MQ=255) agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACTTCgt < 1:1801348/69‑1 (MQ=255) agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgt > 1:3103965/1‑69 (MQ=255) agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgt > 1:934119/1‑69 (MQ=255) agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgt > 1:1396066/1‑69 (MQ=255) agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgt > 1:62779/1‑69 (MQ=255) agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgt > 1:149737/1‑69 (MQ=255) agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgt > 1:1870987/1‑69 (MQ=255) agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgt > 1:3324491/1‑69 (MQ=255) agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgt > 1:1983150/1‑69 (MQ=255) agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgt > 1:2131981/1‑69 (MQ=255) agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgt > 1:2763180/1‑69 (MQ=255) gtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACTTCgt < 1:2002580/68‑1 (MQ=255) ttCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCTGCGATCACTTCAGAGACTTCgt < 1:1105190/67‑1 (MQ=255) tCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgtgt > 1:148066/1‑68 (MQ=255) tCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgtgt > 1:104222/1‑68 (MQ=255) | GACAATGTCACTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCGTGT > minE/1884012‑1884144 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |