| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,884,138 | A→T | 35.8% | D115E (GAT→GAA) | rumA ← | 23S rRNA (uracil‑5)‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,884,138 | 0 | A | T | 35.8% | 78.5 / 75.7 | 92 | D115E (GAT→GAA) | rumA | 23S rRNA (uracil‑5)‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (35/24); new base T (20/13); total (55/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
AGTTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTAAATCCACGCTGG > minE/1884074‑1884199 | agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACTTCgt < 1:2736841/69‑1 (MQ=255)agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACTTCgt < 1:1440012/69‑1 (MQ=255)agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACTTCgt < 1:3340140/69‑1 (MQ=255)agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACTTCgt < 1:1801348/69‑1 (MQ=255)agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACTTCgt < 1:1916190/69‑1 (MQ=255)agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACTTCgt < 1:748581/69‑1 (MQ=255)agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACTTCgt < 1:2214374/69‑1 (MQ=255)agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACTTCgt < 1:2940086/69‑1 (MQ=255)agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACTTCgt < 1:2763544/69‑1 (MQ=255)agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACTTCgt < 1:1068538/69‑1 (MQ=255)agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgt > 1:2131981/1‑69 (MQ=255)agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgt > 1:1983150/1‑69 (MQ=255)agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgt > 1:3103965/1‑69 (MQ=255)agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgt > 1:1870987/1‑69 (MQ=255)agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgt > 1:2763180/1‑69 (MQ=255)agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgt > 1:3324491/1‑69 (MQ=255)agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgt > 1:149737/1‑69 (MQ=255)agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgt > 1:1396066/1‑69 (MQ=255)agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgt > 1:934119/1‑69 (MQ=255)agtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgt > 1:62779/1‑69 (MQ=255) gtTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACTTCgt < 1:2002580/68‑1 (MQ=255) ttCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCTGCGATCACTTCAGAGACTTCgt < 1:1105190/67‑1 (MQ=255) tCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgtgt > 1:104222/1‑68 (MQ=255) tCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCgtgt > 1:148066/1‑68 (MQ=255) gcgcgACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCg < 1:2256833/69‑1 (MQ=255) gcgcgACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCg < 1:2206805/69‑1 (MQ=255) gcgcgACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCg < 1:2176334/69‑1 (MQ=255) gcgcgACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCg < 1:1006742/69‑1 (MQ=255) gcgcgACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCg < 1:1165153/69‑1 (MQ=255) gcgcgACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCg < 1:651224/69‑1 (MQ=255) gcgcgACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCg < 1:3225003/69‑1 (MQ=255) gcgcgACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCg < 1:3337265/69‑1 (MQ=255) gcgcgACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCg < 1:1754574/69‑1 (MQ=255) gcgcgACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCg < 1:1632028/69‑1 (MQ=255) gcgcgACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCg < 1:1502153/69‑1 (MQ=255) cgcgACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCg < 1:1282954/68‑1 (MQ=255) cgcgACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCg < 1:2207349/68‑1 (MQ=255) gcgACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCGAg < 1:648447/69‑1 (MQ=255) gcgACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCGAg < 1:2565658/69‑1 (MQ=255) gACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACTTCgt < 1:2884465/48‑1 (MQ=255) aGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACttt < 1:895394/69‑1 (MQ=255) aGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACttt < 1:2048087/69‑1 (MQ=255) aGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACttt < 1:2267873/69‑1 (MQ=255) aGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACttt < 1:3276895/69‑1 (MQ=255) gAACATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCgct > 1:833333/1‑69 (MQ=255) gAACATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCgc > 1:1119229/1‑68 (MQ=255) aaCATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCgctg > 1:3288065/1‑69 (MQ=255) aaCATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCgctg > 1:537751/1‑69 (MQ=255) aCATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCgctgc > 1:2797297/1‑69 (MQ=255) aCATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCgctgc > 1:3050310/1‑69 (MQ=255) aCATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCgctgc > 1:2401539/1‑69 (MQ=255) aCATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCgctgc > 1:2579957/1‑69 (MQ=255) aCATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGAGCCGCACTTTTGCTTCgctgc > 1:1046736/1‑69 (MQ=255) aCATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATAGGGCGAGTGCCGCACTTTTGCTTCgctgc > 1:2345750/1‑69 (MQ=255) gCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTaa < 1:1873323/68‑1 (MQ=255) gATCACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTAAATc < 1:2460960/69‑1 (MQ=255) gATCACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTAAATc < 1:2098342/69‑1 (MQ=255) gATCACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTAAATc < 1:1973753/69‑1 (MQ=255) gATCACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTAAATc < 1:3410412/69‑1 (MQ=255) cACTTGAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTAAATCCAc > 1:2985766/1‑69 (MQ=255) cACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTAAATCCAc > 1:3318693/1‑69 (MQ=255) cACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTAAATCCAc > 1:1449999/1‑69 (MQ=255) cACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTAAATCCAc > 1:498094/1‑69 (MQ=255) cACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTAAATCCAc > 1:110078/1‑69 (MQ=255) cACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTAAATCCAc > 1:862796/1‑69 (MQ=255) cACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTAAATCCAc > 1:1784317/1‑69 (MQ=255) cACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTAAATCCAc > 1:1098385/1‑69 (MQ=255) cACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTAAATCCAc > 1:2371981/1‑69 (MQ=255) cACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTAAATCCAc > 1:1927127/1‑69 (MQ=255) cACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGAACTTTTGCTTCgc > 1:918441/1‑55 (MQ=255) cAGAGACTTCGTGTTTCATTAATCGGGCGAGTGCCGCAc > 1:969451/1‑39 (MQ=255) cAGAGACTTCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTaa > 1:916400/1‑58 (MQ=255) cAGAGACTTCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTaa > 1:2746864/1‑58 (MQ=255) cAGAGACTTCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTaa > 1:801832/1‑58 (MQ=255) cAGAGACTTCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTaa > 1:1122104/1‑58 (MQ=255) cAGAGACTTCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTaa > 1:745678/1‑58 (MQ=255) cAGAGACTTCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTaa > 1:1230177/1‑58 (MQ=255) cAGAGACTTCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTaa > 1:959739/1‑58 (MQ=255) cAGAGACTTCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTaa > 1:1232756/1‑58 (MQ=255) cAGAGACTTCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTaa > 1:48338/1‑58 (MQ=255) cAGAGACTTCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTaa > 1:367345/1‑58 (MQ=255) cAGAGACTTCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTaa > 1:3359865/1‑58 (MQ=255) cAGAGACTTCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTaa > 1:1639369/1‑58 (MQ=255) cAGAGACTTCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTaa > 1:180343/1‑58 (MQ=255) cAGAGACTTCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTaa > 1:1815199/1‑58 (MQ=255) cAGAGACTTCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTaa > 1:314589/1‑58 (MQ=255) cAGAGACTTCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTaa > 1:229853/1‑58 (MQ=255) cAGAGACTTCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTaa > 1:2403292/1‑58 (MQ=255) cAGAGACTTCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTa > 1:376199/1‑57 (MQ=255) cAGAGACTTCGTGTTTCATTAATCGGGCGAGTGCAGCACTTTTGCTTCGCTGCTGTaa > 1:119667/1‑58 (MQ=255) cAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACtttt > 1:3396934/1‑43 (MQ=255) cAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTAAATCCACGCTgg > 1:2857824/1‑69 (MQ=255) | AGTTCAGACTTAAACGCGCGCGACGGCGATAGCCCCAGGGAACATCGGCGATCACTTCAGAGACATCGTGTTTCATTAATCGGGCGAGTGCCGCACTTTTGCTTCGCTGCTGTAAATCCACGCTGG > minE/1884074‑1884199 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |