| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,885,303 | G→A | 54.0% | Q255Q (CAG→CAA) | barA → | hybrid sensory histidine kinase, in two‑component regulatory system with UvrY |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,885,303 | 0 | G | A | 54.0% | 12.6 / 96.9 | 113 | Q255Q (CAG→CAA) | barA | hybrid sensory histidine kinase, in two‑component regulatory system with UvrY |
| Reads supporting (aligned to +/- strand): ref base G (37/15); new base A (37/24); total (74/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
Only 100 of 128 total aligned reads displayed.
ACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTG > minE/1885251‑1885370 | aCGGTATCAACTCGATGGCAATGTCGCTGGCGGCTTATCACGAAGAGATGCAACACAATATCGACCAg > 1:620218/1‑68 (MQ=255)aCGGTATCAACTCGATGGCAATGTCGCTGGCGGCTTATCACGAAGAGATGCAACACAATATCGACCAg > 1:31404/1‑68 (MQ=255)aCGGTATCAACTCGATGGCAATGTCGCTGGCGGCTTATCACGAAGAGATGCAACACAATATCGACCAg > 1:328271/1‑68 (MQ=255)aCGGTATCAACTCGATGGCAATGTCGCTGGCGGCTTATCACGAAGAGATGCAACACAATATCGACCAg > 1:3083988/1‑68 (MQ=255)aCGGTATCAACTCGATGGCAATGTCGCTGGCGGCTTATCACGAAGAGATGCAACACAATATCGACCAg > 1:2920590/1‑68 (MQ=255)aCGGTATCAACTCGATGGCAATGTCGCTGGCGGCTTATCACGAAGAGATGCAACACAATATCGACCAg > 1:2424815/1‑68 (MQ=255)aCGGTATCAACTCGATGGCAATGTCGCTGGCGGCTTATCACGAAGAGATGCAACACAATATCGACCAg > 1:2345160/1‑68 (MQ=255)aCGGTATCAACTCGATGGCAATGTCGCTGGCGGCTTATCACGAAGAGATGCAACACAATATCGACCAg > 1:229851/1‑68 (MQ=255)aCGGTATCAACTCGATGGCAATGTCGCTGGCGGCTTATCACGAAGAGATGCAACACAATATCGACCAg > 1:581536/1‑68 (MQ=255)aCGGTATCAACTCGATGGCAATGTCGCTGGCGGCTTATCACGAAGAGATGCAACACAATATCGACCAg > 1:1914622/1‑68 (MQ=255)aCGGTATCAACTCGATGGCAATGTCGCTGGCGGCTTATCACGAAGAGATGCAACACAATATCGACCAg > 1:1892410/1‑68 (MQ=255)aCGGTATCAACTCGATGGCAATGTCGCTGGCGGCTTATCACGAAGAGATGCAACACAATATCGACCAg > 1:1642801/1‑68 (MQ=255)aCGGTATCAACTCGATGGCAATGTCGCTGGCGGCTTATCACGAAGAGATGCAACACAATATCGACCAg > 1:856500/1‑68 (MQ=255)aCGGTATCAACTCGATGGCAATGTCGCTGGCGGCTTATCACGAAGAGATGCAACACAATATCGACCAg > 1:1218025/1‑68 (MQ=255)aCGGTATCAACACGATGGCAATGTCGCTGGCGGCTTATCACGAAGAGATGCAACACAATATCGACCAg > 1:381592/1‑68 (MQ=255) ggTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCg < 1:2913718/69‑1 (MQ=255) ggTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCg < 1:2753309/69‑1 (MQ=255) ggTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCg < 1:2553955/69‑1 (MQ=255) aaCTCGATGGCAATGTCGCTGGCTGCTTATCACGATGAGATGCAGCACAATATCGACCAGGc > 1:2540350/1‑62 (MQ=255) aaCTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAat > 1:1209209/1‑51 (MQ=255) aaCTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGc > 1:2966504/1‑62 (MQ=255) aaCTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGc > 1:343718/1‑62 (MQ=255) aaCTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGc > 1:2572742/1‑62 (MQ=255) aaCTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTcc > 1:1681723/1‑69 (MQ=255) aaCTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTcc > 1:2200291/1‑69 (MQ=255) aaCTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTcc > 1:1781816/1‑69 (MQ=255) aaCTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTcc > 1:2974572/1‑69 (MQ=255) aaCTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTcc > 1:265565/1‑69 (MQ=255) aaCTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTcc > 1:1012231/1‑69 (MQ=255) aaTGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGa < 1:3352639/69‑1 (MQ=255) tCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACg > 1:707461/1‑69 (MQ=255) tCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACg > 1:368895/1‑69 (MQ=255) ggctgCTTATCACGAAGTGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTgg > 1:2168026/1‑68 (MQ=255) ggctgCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTgg > 1:2643329/1‑68 (MQ=255) cACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATgg < 1:1089017/67‑1 (MQ=255) cACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGaa > 1:1190963/1‑69 (MQ=255) cACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGaa > 1:858734/1‑69 (MQ=255) cACGAAGAGATGCAACACAATATCGACCAGGCGACg > 1:671252/1‑36 (MQ=255) cACGAAGAGATGCAACACAATATCGACCAGGCGACg > 1:267489/1‑36 (MQ=255) cACGAAGAGATGCAACACAATATCGACCAGGCGACg > 1:1040420/1‑36 (MQ=255) cACGAAGAGATGCAACACAATATCGACCAGGCGACg > 1:1126437/1‑36 (MQ=255) cACGAAGAGATGCAACACAATATCGACCAGGCGACg > 1:2097116/1‑36 (MQ=255) cACGAAGAGATGCAACACAATATCGACCAGGCGACg > 1:114001/1‑36 (MQ=255) cACGAAGAGATGCAACACAATATCGACCAGGCGACg > 1:2942001/1‑36 (MQ=255) cACGAAGAGATGCAACACAATATCGACCAGGCGACg > 1:3042186/1‑36 (MQ=255) cACGAAGAGATGCAACACAATATCGACCAGGCGACg > 1:1542542/1‑36 (MQ=255) aCGAAGAGATGTAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGaaa > 1:1575240/1‑69 (MQ=255) aCGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGaaa > 1:2913878/1‑69 (MQ=255) aCGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGaaa > 1:1034751/1‑69 (MQ=255) aCGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGaaa > 1:1251612/1‑69 (MQ=255) aCGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGaaa > 1:101312/1‑69 (MQ=255) aCGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGaaa > 1:1003889/1‑69 (MQ=255) aCGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGaaa > 1:2585290/1‑69 (MQ=255) aCGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGaaa > 1:2434486/1‑69 (MQ=255) aCGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGaaa > 1:899387/1‑69 (MQ=255) aCGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGaaa > 1:836559/1‑69 (MQ=255) aCGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGaaa > 1:1657254/1‑69 (MQ=255) aCGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGaaa > 1:943706/1‑69 (MQ=255) aCGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGaa > 1:1054027/1‑68 (MQ=255) aCGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGaa > 1:1905180/1‑68 (MQ=255) aCGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGATGGAGCAGATGGaa > 1:1549187/1‑68 (MQ=255) gagaTGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAg < 1:2484796/69‑1 (MQ=255) gagaTGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAg < 1:1273536/69‑1 (MQ=255) gagaTGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAg < 1:2255608/69‑1 (MQ=255) gagaTGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAg < 1:2781407/69‑1 (MQ=255) gagaTGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAg < 1:467104/69‑1 (MQ=255) gagaTGCAACACAATATCGACCAGGCGACGTCCGATCTGCGTGAGACGCTGGAGCAAATGGAGATTCAg < 1:638381/69‑1 (MQ=255) gagaTGCAACACAATATCGACCAGGCGACGTCCGATCTGCGTGAGACGCTGGAGCAAATGGAGATTCAg < 1:34720/69‑1 (MQ=255) gagaTGCAACACAATATCGACCAGGCGACGTCCGATCTGCGTGAGACGCTGGAGCAAATGGAGATTCAg < 1:1011562/69‑1 (MQ=255) gagaTGCAACACAATATCGACCAGGCGACGTCCGATCTGCGTGAGACGCTGGAGCAAATGGAGATTCAg < 1:797858/69‑1 (MQ=255) gagaTGCAACACAATATCGACCAGGCGACGTCCGATCTGCGTGAGACGCTGGAGCAAATGGAGATTCAg < 1:3302086/69‑1 (MQ=255) gagaTGCAACACAATATCGACCAGGCGACGTCCGATCTGCGTGAGACGCTGGAGCAAATGGAGATTCAg < 1:919022/69‑1 (MQ=255) gagaTGCAACACAATATCGACCAGGCGACGTCCGATCTGCGTGAGACGCTGGAGCAAATGGAGATTCAg < 1:1489687/69‑1 (MQ=255) gagaTGCAACACAATATCGACCAGGCGACGTCCGATCTGCGTGAGACGCTGGAGCAAATGGAGATTCAg < 1:2072023/69‑1 (MQ=255) gagaTGCAACACAATATCGACCAGGCGACGTCCGATCTGCGTGAGACGCTGGAGCAAATGGAGATTCAg < 1:1533362/69‑1 (MQ=255) gagaTGCAACACAATATCGACCAGGCGACGTCCGATCTGCGTGAGACGCTGGAGCAAATGGAGATTCAg < 1:2291549/69‑1 (MQ=255) gagaTGCAACACAATATCGACCAGGCGACGTCCGATCTGCGTGAGACGCTGGAGCAAATGGAGATTCAg < 1:2395070/69‑1 (MQ=255) gagaTGCAACACAATATCGACCAGGCGACGTCCGATCTGCGTGAGACGCTGGAGCAAATGGAGATTCAg < 1:2766328/69‑1 (MQ=255) gagaTGCAACACAATATCGACCAGGCGACGTCCGATCTGCGTGAGACGCTGGAGCAAATGGAGATTCAg < 1:3033006/69‑1 (MQ=255) gagaTGCAACACAATATCGACCAGGCGACGTCCGATCTGCGTGAGACGCTGGAGCAAATGGAGATTCAg < 1:3042889/69‑1 (MQ=255) gagaTGCAACACAATATCGACCAGGCGACGTCCGATCTGCGTGAGACGCTGGAGCAAATGGAGATTCAg < 1:1281781/69‑1 (MQ=255) gagaTGCAACACAATATCGACCAGGCGACGTCCGATCTGCGTGAGACGCTGGAGCAAATGGAGATTCAg < 1:3103116/69‑1 (MQ=255) agaTGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAg < 1:2896964/68‑1 (MQ=255) agaTGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGa > 1:3042839/1‑69 (MQ=255) agaTGCAACACAATATCGACCAGGCGACGTCCGATCTGCGTGAGACGCTGGAGCAAATGGAGATTCAg < 1:487750/68‑1 (MQ=255) agaTGCAACACAATATCGACCAGGCCACGTCCGATCTGCGTGAGACGCTGGAGCAAATGGAGATTCAg < 1:635745/68‑1 (MQ=255) cagcaCAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGa < 1:1483329/56‑1 (MQ=255) cagcaCAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGa < 1:943820/56‑1 (MQ=255) cagcaCAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGa < 1:1213445/56‑1 (MQ=255) cagcaCAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGa < 1:3220265/56‑1 (MQ=255) cagcaCAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGa < 1:1995382/56‑1 (MQ=255) cagcaCAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGa < 1:357507/56‑1 (MQ=255) cagcaCAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGa < 1:3130186/56‑1 (MQ=255) gcaCAATATCGACCAGGCGACGTCCGATCTGCGTTAAACGCTGGAGCAGATGGAAATTCAGAACGTTg < 1:3319507/68‑1 (MQ=255) gcaCAATATCGACCAGGCGACGTCCGATCTGCGTGAAAGGCTGGAGCAGATGGAAATTCAGAACGTTg < 1:2121460/68‑1 (MQ=255) gcaCAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTg < 1:1900904/68‑1 (MQ=255) gcaCAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTg < 1:1518173/68‑1 (MQ=255) gcaCAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTg < 1:3411190/68‑1 (MQ=255) gcaCAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTg < 1:3324607/68‑1 (MQ=255) gcaCAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTg < 1:1149039/68‑1 (MQ=255) | ACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTG > minE/1885251‑1885370 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |