| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,886,479 | G→A | 42.8% | T647T (ACG→ACA) | barA → | hybrid sensory histidine kinase, in two‑component regulatory system with UvrY |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,886,479 | 0 | G | A | 42.8% | 25.0 / 56.5 | 77 | T647T (ACG→ACA) | barA | hybrid sensory histidine kinase, in two‑component regulatory system with UvrY |
| Reads supporting (aligned to +/- strand): ref base G (17/27); new base A (10/23); total (27/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01 | |||||||||||
GGTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGT > minE/1886420‑1886547 | ggTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGt < 1:1016376/69‑1 (MQ=255)ggTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGt < 1:2967943/69‑1 (MQ=255)ggTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGt < 1:3144744/69‑1 (MQ=255)ggTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGt < 1:3249181/69‑1 (MQ=255)ggTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGt < 1:1986022/69‑1 (MQ=255)ggTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGt < 1:1903604/69‑1 (MQ=255) gTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGt < 1:3312959/68‑1 (MQ=255) gTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGt < 1:1386143/68‑1 (MQ=255) aTCGGCGCGTGTCTGCTGAAACCATTAACACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTGtcat < 1:1075248/69‑1 (MQ=255) aTCGGCGCGTGTCTGCTGAAACCATTAACACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTGtcat < 1:239624/69‑1 (MQ=255) aTCGGCGCGTGTCTGCTGAAACCATTAACACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTGtcat < 1:3096685/69‑1 (MQ=255) aTCGGCGCGTGTCTGCTGAAACCATTAACACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTGtcat < 1:3124107/69‑1 (MQ=255) aTCGGCGCGTGTCTGCTGAAACCATTAACACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTGtcat < 1:867339/69‑1 (MQ=255) aTCGGCGCGTGTCTGCTGAAACCATTAACACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTGtcat < 1:775360/69‑1 (MQ=255) gcgTGTCTGCTGAAACCATTAACACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTGtcat < 1:1958330/63‑1 (MQ=255) ctgctgAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGt < 1:37205/54‑1 (MQ=255) gAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCt < 1:2947058/69‑1 (MQ=255) gAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCt < 1:317822/69‑1 (MQ=255) gAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCt < 1:3176316/69‑1 (MQ=255) gAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCt < 1:2008658/69‑1 (MQ=255) gAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCt < 1:1045466/69‑1 (MQ=255) gAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCt > 1:10171/1‑69 (MQ=255) gAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCt < 1:2212349/69‑1 (MQ=255) gAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCt > 1:2258933/1‑69 (MQ=255) gAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCt < 1:1187058/69‑1 (MQ=255) gAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCt > 1:2416872/1‑69 (MQ=255) gAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCt > 1:161824/1‑69 (MQ=255) gAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCt > 1:1581125/1‑69 (MQ=255) gAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCt < 1:2538935/69‑1 (MQ=255) ccATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTg < 1:1982318/69‑1 (MQ=255) ccATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTg < 1:2551079/69‑1 (MQ=255) ttAACACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTGTCATCACAAACAAAACACGCTTTTGCCt < 1:602692/69‑1 (MQ=255) ttAACACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTGTCATCACAAACAAAACACGCTTTTGCCt < 1:1240590/69‑1 (MQ=255) ttAACACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTGTCATCACAAACAAAACACGCTTTTGCCt < 1:2863490/69‑1 (MQ=255) ttAACACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTGTCATCACAAACAAAACACGCTTTTGCCt < 1:1280492/69‑1 (MQ=255) ttAACACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTGTCATCACAAACAAAACACGCTTTTGCCt < 1:2531791/69‑1 (MQ=255) ttAACACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTGTCATCACAAACAAAACACGCTTTTGCCt < 1:10044/69‑1 (MQ=255) ttAACACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTGTCATCACAAACAAAACACGCTTTTGCCt < 1:1065199/69‑1 (MQ=255) ttAACACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTGTCATCACAAACAAAACACGCTTTTGCCt < 1:3284754/69‑1 (MQ=255) ttAACACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTGTCATCACAAACAAAACACGCTTTTGCCt < 1:745618/69‑1 (MQ=255) ttAACACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTGTCATCACAAACAAAACACGCTTTTGCCt < 1:922288/69‑1 (MQ=255) ttAACACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTGTCATCACAAACAAAACACGCTTTTGCCt < 1:1895612/69‑1 (MQ=255) ttAACACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTGTCATCACAAACAAAACACGCTTTTGCCt < 1:337763/69‑1 (MQ=255) ttAACACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTGTCATCACAAACAAAACACGCTTTTGCCt < 1:352694/69‑1 (MQ=255) ttAACACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTGTCATCACAAACAAAACACGCTATTGCCt < 1:440434/69‑1 (MQ=255) ttAAAACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTGTCATCACAAACAAAACACGCTTTTGCCt < 1:689859/69‑1 (MQ=255) aaCACGTACGCGCCTGTTGCCTGCCCTGACAGAATTTTg > 1:2898071/1‑39 (MQ=37) aaCACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTg > 1:2561926/1‑39 (MQ=38) aaCACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTg > 1:512951/1‑39 (MQ=38) aaCACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTg > 1:266751/1‑39 (MQ=38) aaCACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTg > 1:357094/1‑39 (MQ=38) aaCACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTg > 1:997750/1‑39 (MQ=38) aaCACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTg > 1:3280675/1‑39 (MQ=38) aaCACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTg > 1:1360380/1‑39 (MQ=38) aaCACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTg > 1:202002/1‑39 (MQ=38) aaCACCTACGCGCCTGTTGCCTGCCCTGACAGAATTTTg > 1:2016002/1‑39 (MQ=38) acacCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTg < 1:1061388/63‑1 (MQ=255) ccTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAAcc < 1:1955496/69‑1 (MQ=255) tACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACaaacaaaaca > 1:722600/1‑50 (MQ=255) tACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACaaacaaaaca > 1:133643/1‑50 (MQ=255) tACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACaaacaaaaca > 1:2229162/1‑50 (MQ=255) tACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACaaacaaaaca > 1:884061/1‑50 (MQ=255) tACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACaaacaaaaca > 1:3140972/1‑50 (MQ=255) tACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACaaacaaaaca > 1:2181997/1‑50 (MQ=255) tACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACaaacaaaaca > 1:826598/1‑50 (MQ=255) tACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACaaacaaaaca > 1:1299557/1‑50 (MQ=255) tACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTa < 1:483617/64‑1 (MQ=255) tACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTa < 1:660037/64‑1 (MQ=255) tACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTa < 1:2552570/64‑1 (MQ=255) tACACGACTGTTGCCTGCCCTGACGGAATTTTGTCATCACaaacaaaaca > 1:439474/1‑50 (MQ=255) acgcGCCTGTTGCCTGCCCTGACAGAATTTTGTCATCACAAACAAAACACGCTTTTGCCt < 1:1625896/57‑1 (MQ=255) cacGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGAt > 1:2209107/1‑68 (MQ=255) cacGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGAt > 1:2383965/1‑68 (MQ=255) cacGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGAt > 1:2488722/1‑68 (MQ=255) cGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGaa < 1:2073402/69‑1 (MQ=255) cGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGaa < 1:1688121/69‑1 (MQ=255) cGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGaa < 1:645577/69‑1 (MQ=255) ggAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGt < 1:1353374/69‑1 (MQ=255) ggAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGt < 1:952056/69‑1 (MQ=255) ggAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGt < 1:1389750/69‑1 (MQ=255) ggAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGt < 1:1449811/69‑1 (MQ=255) ggAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGt < 1:103955/69‑1 (MQ=255) ggAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGt < 1:488325/69‑1 (MQ=255) ggAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGt < 1:470017/69‑1 (MQ=255) ggAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGt < 1:359939/69‑1 (MQ=255) ggAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGt < 1:1680614/69‑1 (MQ=255) ggAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGt < 1:3385978/69‑1 (MQ=255) ggAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGt < 1:1864314/69‑1 (MQ=255) ggAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGt < 1:1918686/69‑1 (MQ=255) ggAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGt < 1:3285773/69‑1 (MQ=255) ggAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGt < 1:1973493/69‑1 (MQ=255) ggAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGt < 1:3236339/69‑1 (MQ=255) ggAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGt < 1:2223924/69‑1 (MQ=255) ggAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGt < 1:2419489/69‑1 (MQ=255) ggAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGt < 1:2895754/69‑1 (MQ=255) ggAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGt < 1:2441524/69‑1 (MQ=255) ggAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGAAAGt < 1:1656325/69‑1 (MQ=255) | GGTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGT > minE/1886420‑1886547 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |