| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,886,680 | G→A | 16.9% | L714L (TTG→TTA) | barA → | hybrid sensory histidine kinase, in two‑component regulatory system with UvrY |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,886,680 | 0 | G | A | 16.9% | 145.2 / 16.0 | 88 | L714L (TTG→TTA) | barA | hybrid sensory histidine kinase, in two‑component regulatory system with UvrY |
| Reads supporting (aligned to +/- strand): ref base G (36/37); new base A (6/9); total (42/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.42e-01 | |||||||||||
CAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCA > minE/1886612‑1886742 | cAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGGCGTTCGATTTg < 1:2555889/69‑1 (MQ=255)cAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTg < 1:2314745/69‑1 (MQ=255)cAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTg < 1:1431177/69‑1 (MQ=255)cAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTg < 1:3285816/69‑1 (MQ=255)cAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTg < 1:2658462/69‑1 (MQ=255)cAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTg < 1:2522285/69‑1 (MQ=255)cAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTg < 1:2405764/69‑1 (MQ=255)cAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTg < 1:2403392/69‑1 (MQ=255) tgtgGAACTTTGCGATAGCGGTCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCtt < 1:1864716/69‑1 (MQ=255) tgtgGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCtt > 1:175151/1‑69 (MQ=255) tgtgGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCtt < 1:2773389/69‑1 (MQ=255) tgtgGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCtt > 1:2788341/1‑69 (MQ=255) tgtgGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCtt < 1:2900377/69‑1 (MQ=255) tgtgGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCtt < 1:30440/69‑1 (MQ=255) tgtgGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCtt > 1:3076917/1‑69 (MQ=255) tgtgGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCtt > 1:3112790/1‑69 (MQ=255) tgtgGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCtt > 1:3093201/1‑69 (MQ=255) tgtgGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCtt > 1:3118622/1‑69 (MQ=255) tgtgGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCtt > 1:1396276/1‑69 (MQ=255) tgtgGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCtt < 1:1291759/69‑1 (MQ=255) tgtgGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCtt > 1:3296869/1‑69 (MQ=255) tgtgGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCtt > 1:3346130/1‑69 (MQ=255) tgtgGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCtt > 1:539714/1‑69 (MQ=255) tgtgGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCtt < 1:770576/69‑1 (MQ=255) tgtgGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCtt > 1:773791/1‑69 (MQ=255) tgtgGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCtt > 1:930591/1‑69 (MQ=255) tgtgGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCtt > 1:960248/1‑69 (MQ=255) tgtgGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGACGCCGTTCGATTTGATCtt > 1:3150298/1‑69 (MQ=255) tgtgGAACTTTGCGATAGCGGCCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCtt > 1:1232419/1‑69 (MQ=255) tgtgGAACTTTGAGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCtt > 1:1351006/1‑69 (MQ=255) tgGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTaa > 1:995386/1‑69 (MQ=255) tgGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTaa > 1:2619468/1‑69 (MQ=255) tgGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTaa > 1:152305/1‑69 (MQ=255) tgGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTaa > 1:162218/1‑69 (MQ=255) gAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTAATCTTAATg > 1:1936589/1‑69 (MQ=255) gAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTAATCTTAATg > 1:1993709/1‑69 (MQ=255) gAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTAATCTTAATg > 1:1411513/1‑69 (MQ=255) gAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTAATCTTAATg > 1:2244155/1‑69 (MQ=255) gAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTAATCTTAATg > 1:2692700/1‑69 (MQ=255) gAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTAATCTTAATg > 1:173884/1‑69 (MQ=255) aCTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGa < 1:1018544/69‑1 (MQ=255) aCTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGa < 1:2932802/69‑1 (MQ=255) aCTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGa < 1:2930853/69‑1 (MQ=255) gATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCa < 1:1270932/68‑1 (MQ=255) gATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCa < 1:2968004/68‑1 (MQ=255) gATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCa < 1:2357471/68‑1 (MQ=255) gATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCa < 1:1864517/68‑1 (MQ=255) gCGGGCATCAGGCGGTTGAACGGGCGACACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGc < 1:2810099/69‑1 (MQ=255) gCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGc < 1:2422382/69‑1 (MQ=255) cAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACatg < 1:3292393/69‑1 (MQ=255) cAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACatg < 1:1318013/69‑1 (MQ=255) ggCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACatgga > 1:617664/1‑69 (MQ=255) gCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCtt < 1:2818632/41‑1 (MQ=255) ggTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACatggatgg > 1:1776777/1‑69 (MQ=255) ggTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACatggatgg < 1:113732/69‑1 (MQ=255) ggTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACatggatgg < 1:3103191/69‑1 (MQ=255) ggTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACatggatgg < 1:1129391/69‑1 (MQ=255) ggTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACatggatgg > 1:1114110/1‑69 (MQ=255) gTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACatg < 1:1917632/63‑1 (MQ=255) gTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACatg < 1:222256/63‑1 (MQ=255) gTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACatg < 1:3323870/63‑1 (MQ=255) gTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGc > 1:1469787/1‑69 (MQ=255) gTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGc > 1:325098/1‑69 (MQ=255) gTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGc > 1:796494/1‑69 (MQ=255) gTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGc > 1:3305044/1‑69 (MQ=255) gTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGc > 1:1030962/1‑69 (MQ=255) gTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGc > 1:562363/1‑69 (MQ=255) ttGAACGGGCGAAACAGCTGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCa < 1:608912/69‑1 (MQ=255) ttGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCa < 1:2800866/69‑1 (MQ=255) tGAACGGGCGAAACAGATGCCGTTCGATTTAATCtt < 1:3435861/36‑1 (MQ=255) tGAACGGGCGAAACAGATGCCGTTCGATTTAATCtt < 1:956722/36‑1 (MQ=255) tGAACGGGCGAAACAGATGCCGTTCGATTTAATCtt < 1:789571/36‑1 (MQ=255) tGAACGGGCGAAACAGATGCCGTTCGATTTAATCtt < 1:1956068/36‑1 (MQ=255) tGAACGGGCGAAACAGATGCCGTTCGATTTAATCtt < 1:1573462/36‑1 (MQ=255) tGAACGGGCGAAACAGATGCCGTTCGATTTAATCtt < 1:1870783/36‑1 (MQ=255) tGAACGGGCGAAACAGATGCCGTTCGATTTAATCtt < 1:2582412/36‑1 (MQ=255) tGAACGGGCGAAACAGATGCCGTTCGATGTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCag > 1:2466575/1‑68 (MQ=255) tGAAAGGGCGAAACAGATGCCGTTCGATTTAATCtt < 1:1694066/36‑1 (MQ=38) gAACGGGCGAAACAGATGCCGTTCGATTTAATCtt < 1:2834596/35‑1 (MQ=255) ggCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGc > 1:974037/1‑69 (MQ=255) ggCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGc > 1:2537667/1‑69 (MQ=255) ggCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGc > 1:1162431/1‑69 (MQ=255) ggCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGc > 1:1634407/1‑69 (MQ=255) ggCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGCACATGGATGGCATTCGGGc > 1:1155583/1‑69 (MQ=255) aCAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGc < 1:3289303/63‑1 (MQ=255) aCAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGc < 1:974055/63‑1 (MQ=255) aCAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGc < 1:1730464/63‑1 (MQ=255) aCAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGc < 1:777658/63‑1 (MQ=255) aCAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGc < 1:381097/63‑1 (MQ=255) aCAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGc < 1:157662/63‑1 (MQ=255) tGCCGTTCGATTTGATCTTAATGGATATTCAAATGc < 1:1814919/36‑1 (MQ=255) cGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATccacca < 1:1133244/69‑1 (MQ=255) cGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATccacca < 1:286365/69‑1 (MQ=255) cGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATccacca > 1:2972806/1‑69 (MQ=255) cGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATccacca < 1:3045816/69‑1 (MQ=255) tttGATCTTAATGGATATTCAAATGCCGGACATGGATGGCa < 1:3066213/41‑1 (MQ=255) | CAACATGTGGAACTTTGCGATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCA > minE/1886612‑1886742 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |