| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,887,935 | C→A | 37.1% | L311L (CTG→CTT) | gudD ← | (D)‑glucarate dehydratase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,887,935 | 0 | C | A | 37.1% | 96.7 / 81.6 | 105 | L311L (CTG→CTT) | gudD | (D)‑glucarate dehydratase 1 |
| Reads supporting (aligned to +/- strand): ref base C (39/27); new base A (12/27); total (51/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.23e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01 | |||||||||||
Only 100 of 114 total aligned reads displayed.
TTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATT > minE/1887875‑1888003 | ttCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGat > 1:3247214/1‑69 (MQ=255) gCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCaa > 1:1507008/1‑68 (MQ=255) gCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCaa > 1:2862298/1‑68 (MQ=255) cacaTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACg > 1:1490118/1‑69 (MQ=255) cacaTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACg > 1:10300/1‑69 (MQ=255) cacaTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACg > 1:1457412/1‑69 (MQ=255) cacaTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACg > 1:1283844/1‑69 (MQ=255) cacaTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACg > 1:1222247/1‑69 (MQ=255) acaTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACgg < 1:2536934/69‑1 (MQ=255) acaTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACgg < 1:218632/69‑1 (MQ=255) acaTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACgg < 1:2160125/69‑1 (MQ=255) acaTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACgg < 1:588231/69‑1 (MQ=255) acaTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACgg < 1:2070480/69‑1 (MQ=255) acaTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACgg < 1:2722505/69‑1 (MQ=255) acaTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACgg < 1:1885151/69‑1 (MQ=255) acaTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACgg < 1:1934038/69‑1 (MQ=255) acaTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACgg < 1:1983077/69‑1 (MQ=255) acaTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACgg < 1:2024260/69‑1 (MQ=255) acaTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACgg < 1:1350759/69‑1 (MQ=255) acaTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACgg < 1:2261900/69‑1 (MQ=255) acaTTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACgg > 1:1148730/1‑69 (MQ=255) acaTTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACgg > 1:2046989/1‑69 (MQ=255) acaTTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACgg > 1:42747/1‑69 (MQ=255) acaTTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACgg > 1:2698767/1‑69 (MQ=255) acaTTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACgg > 1:490845/1‑69 (MQ=255) acaTTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACgg > 1:1208729/1‑69 (MQ=255) acaTTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACgg > 1:1698942/1‑69 (MQ=255) acaTTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACgg > 1:3348935/1‑69 (MQ=255) acaTTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACgg > 1:2905838/1‑69 (MQ=255) acaTTTGCGCCACACGTACCGAACCTTGCATCGTCCAGAAATGCGGATCCGCAAGCGGGATATCAACgg > 1:755405/1‑69 (MQ=255) tGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGc > 1:3092619/1‑69 (MQ=255) tGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGc > 1:3383785/1‑69 (MQ=255) cGTACCGAACCTTGCATTGTCCAGATATGCGGATCCGCCAg > 1:1079303/1‑41 (MQ=255) cGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCTGGATATCAACGGa > 1:1861792/1‑56 (MQ=255) cGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGagag > 1:2528698/1‑68 (MQ=255) cGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGagag > 1:565400/1‑68 (MQ=255) cGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGagag > 1:2393195/1‑68 (MQ=255) cGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGagag > 1:2760364/1‑68 (MQ=255) cGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGagag > 1:2578854/1‑68 (MQ=255) cGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGagag > 1:1777985/1‑68 (MQ=255) cGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGagag > 1:176296/1‑68 (MQ=255) cGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGagag > 1:160540/1‑68 (MQ=255) cGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGagag > 1:307499/1‑68 (MQ=255) cGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGagag > 1:839407/1‑68 (MQ=255) cGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGagag > 1:1143159/1‑68 (MQ=255) cGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGagag > 1:3216149/1‑68 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCg < 1:246588/69‑1 (MQ=255) gTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCg < 1:867868/69‑1 (MQ=255) agcGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACgg < 1:3230040/50‑1 (MQ=255) aCCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTa < 1:1546502/69‑1 (MQ=255) tGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTgcc > 1:3222960/1‑68 (MQ=255) tGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTgcc > 1:3040336/1‑68 (MQ=255) tGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTgcc > 1:2424221/1‑68 (MQ=255) tGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTgcc > 1:1918155/1‑68 (MQ=255) tGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTgcc > 1:1108124/1‑68 (MQ=255) tGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTgcc > 1:1562955/1‑68 (MQ=255) tGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTgcc > 1:698848/1‑68 (MQ=255) tGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTgcc > 1:1501800/1‑68 (MQ=255) tGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGG‑CCATTTgcc > 1:1347234/1‑67 (MQ=255) tGTCCAGAAATGCGGATCCGCCAGCGGGATATCAAAGGATTGCAGGGAGAGCGTATGGCCCATTTgcc > 1:2250498/1‑68 (MQ=255) gTCCAGAAATGCGGATCCGCCAGCGGGATATCAACg < 1:3261663/36‑1 (MQ=255) cAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAg > 1:221364/1‑69 (MQ=255) tGCGGATCCGCAAGCGGGATATCTACGGATTGCAACGAGAGCGTATGGCCCATCTGCCGCCAGTCGGTg < 1:2078504/69‑1 (MQ=255) tGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGAGAGCGTATGGCCCATCTGCCGCCATTCGGTg < 1:6967/69‑1 (MQ=255) tGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGAGAGCGTATGGCCCATCTGCCGCCAGTCGGTg < 1:2388054/69‑1 (MQ=255) tGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGAGAGCGTATGGCCCATCTGCCGCCAGTCGGTg < 1:913751/69‑1 (MQ=255) tGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGAGAGCGTATGGCCCATCTGCCGCCAGTCGGTg < 1:860401/69‑1 (MQ=255) tGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGAGAGCGTATGGCCCATCTGCCGCCAGTCGGTg < 1:761242/69‑1 (MQ=255) tGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGAGAGCGTATGGCCCATCTGCCGCCAGTCGGTg < 1:3253871/69‑1 (MQ=255) tGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGAGAGCGTATGGCCCATCTGCCGCCAGTCGGTg < 1:688476/69‑1 (MQ=255) tGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGAGAGCGTATGGCCCATCTGCCGCCAGTCGGTg < 1:662518/69‑1 (MQ=255) tGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGAGAGCGTATGGCCCATCTGCCGCCAGTCGGTg < 1:582516/69‑1 (MQ=255) tGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGAGAGCGTATGGCCCATCTGCCGCCAGTCGGTg < 1:1046581/69‑1 (MQ=255) tGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGAGAGCGTATGGCCCATCTGCCGCCAGTCGGTg < 1:1047590/69‑1 (MQ=255) tGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGAGAGCGTATGGCCCATCTGCCGCCAGTCGGTg < 1:3150522/69‑1 (MQ=255) tGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGAGAGCGTATGGCCCATCTGCCGCCAGTCGGTg < 1:2802384/69‑1 (MQ=255) tGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGAGAGCGTATGGCCCATCTGCCGCCAGTCGGTg < 1:2465786/69‑1 (MQ=255) tGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGAGAGCGTATGGCCCATCTGCCGCCAGTCGGTg < 1:1532014/69‑1 (MQ=255) tGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGAGAGCGTATGGCCCATCTGCCGCCAGTCGGTg < 1:1001135/69‑1 (MQ=255) tGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGAGAGCGTATGGCCCATCTGCCGCCAGTCGGTg < 1:1743488/69‑1 (MQ=255) tGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGAGAGCGTATGGCCCATCTGCCGCCAGTCGGTg < 1:1882652/69‑1 (MQ=255) tGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGAGAGCGTATGGCCCATCTGCCGCCAGTCGGTg < 1:193865/69‑1 (MQ=255) tGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGAGAGCGTATGGCCCATCTGCCGCCAGTCGGTg < 1:2131451/69‑1 (MQ=255) tGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGAGAGCGTATGGCCCATCTGCCGCCAGTCGGTg < 1:2116415/69‑1 (MQ=255) tGCGGATCCGCAAGCGGGATATCAACGGATTGCAACGAGAGCGTATGGCCCATCTGCCGCCAGTCGGTg < 1:2037082/69‑1 (MQ=255) aTCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGAt < 1:767663/69‑1 (MQ=255) ccGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCa < 1:1016165/69‑1 (MQ=255) ccGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCa < 1:1324418/69‑1 (MQ=255) ccGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCa < 1:2232777/69‑1 (MQ=255) ccGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCa < 1:3329995/69‑1 (MQ=255) ccGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCa < 1:3234866/69‑1 (MQ=255) cGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCa < 1:3238789/68‑1 (MQ=255) cAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATAtt < 1:231796/69‑1 (MQ=255) cAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATAtt < 1:413907/69‑1 (MQ=255) cAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATAtt < 1:3352354/69‑1 (MQ=255) cAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATAtt < 1:1530434/69‑1 (MQ=255) cAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATAtt < 1:1403620/69‑1 (MQ=255) cAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATAtt < 1:800762/69‑1 (MQ=255) cAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATAtt < 1:2806998/69‑1 (MQ=255) cAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATAtt < 1:3217921/69‑1 (MQ=255) | TTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATT > minE/1887875‑1888003 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |