| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,888,954 | A→G | 30.5% | G425G (GGT→GGC) | gudX ← | predicted glucarate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,888,954 | 0 | A | G | 30.5% | 75.6 / 40.6 | 85 | G425G (GGT→GGC) | gudX | predicted glucarate dehydratase |
| Reads supporting (aligned to +/- strand): ref base A (20/39); new base G (12/14); total (32/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01 | |||||||||||
ACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTT > minE/1888894‑1889018 | aCGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGcgtc < 1:1783848/69‑1 (MQ=255)aCGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGcgtc < 1:2975183/69‑1 (MQ=255)aCGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGcgtc < 1:367927/69‑1 (MQ=255)aCGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGcgtc < 1:2128935/69‑1 (MQ=255)aCGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGcgtc < 1:2776483/69‑1 (MQ=255)aCGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGcgtc < 1:465764/69‑1 (MQ=255)aCGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGcgtc < 1:628957/69‑1 (MQ=255) cGGCCGAAACCGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGcgtc < 1:135955/68‑1 (MQ=255) cGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGcgtc < 1:1556476/68‑1 (MQ=255) aaCGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACgcgc < 1:2784576/69‑1 (MQ=255) aaCGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACgcgc < 1:335717/69‑1 (MQ=255) aaCGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACgcgc < 1:2374024/69‑1 (MQ=255) aCGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACgcgc < 1:2778802/68‑1 (MQ=255) aCGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACgcgc < 1:983783/68‑1 (MQ=255) gTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCa > 1:2165901/1‑68 (MQ=255) gTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCa > 1:33660/1‑68 (MQ=255) gTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCa > 1:2377502/1‑68 (MQ=255) gTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCa > 1:2431163/1‑68 (MQ=255) gTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCa > 1:2081963/1‑68 (MQ=255) gTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCa > 1:321142/1‑68 (MQ=255) gTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCa > 1:1768305/1‑68 (MQ=255) gTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCa > 1:2980339/1‑68 (MQ=255) gTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCa > 1:1585310/1‑68 (MQ=255) gTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCa > 1:763659/1‑68 (MQ=255) gTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCa > 1:140778/1‑68 (MQ=255) gTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCa > 1:3347690/1‑68 (MQ=255) gTTTAAGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACgcgc < 1:1496945/61‑1 (MQ=255) tttACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGcgtc < 1:1890572/51‑1 (MQ=255) tACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGc > 1:484785/1‑68 (MQ=255) tACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCa > 1:3311593/1‑69 (MQ=255) tACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCa > 1:3297480/1‑69 (MQ=255) tACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCa > 1:1236365/1‑69 (MQ=255) cAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGtt < 1:2842158/69‑1 (MQ=255) cAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGtt < 1:3371104/69‑1 (MQ=255) cAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGtt < 1:2179796/69‑1 (MQ=255) cAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGtt < 1:367121/69‑1 (MQ=255) cAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGtt < 1:1517549/69‑1 (MQ=255) cAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGtt < 1:1016616/69‑1 (MQ=255) aGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTta < 1:2365232/68‑1 (MQ=255) aGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTta < 1:2162310/68‑1 (MQ=255) aGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTta < 1:1874146/68‑1 (MQ=255) aGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTta < 1:1693052/68‑1 (MQ=255) aGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTta < 1:2958425/68‑1 (MQ=255) ccGGGTATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCa < 1:1449074/69‑1 (MQ=255) ccGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCa < 1:995078/69‑1 (MQ=255) ccGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCa < 1:312088/69‑1 (MQ=255) ccGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCa < 1:3042401/69‑1 (MQ=255) ccGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCa < 1:457425/69‑1 (MQ=255) ccGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCa < 1:854354/69‑1 (MQ=255) ccGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCa < 1:1662043/69‑1 (MQ=255) ccGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCa < 1:1929034/69‑1 (MQ=255) ccGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCa < 1:2523162/69‑1 (MQ=255) ccGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCa < 1:2196358/69‑1 (MQ=255) ccGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCa < 1:2304509/69‑1 (MQ=255) ccGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCa < 1:1571333/69‑1 (MQ=255) ccGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCa < 1:1625890/69‑1 (MQ=255) ccGGGGATCAGGTACTGCATCGGGCCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCa < 1:1612306/69‑1 (MQ=255) ggggATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCAtg < 1:891500/69‑1 (MQ=255) gATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGc > 1:945982/1‑69 (MQ=255) aTCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTta < 1:1665057/54‑1 (MQ=255) tCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCt > 1:234320/1‑69 (MQ=255) tCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCt > 1:3123872/1‑69 (MQ=255) tCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCt > 1:2296560/1‑69 (MQ=255) tCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCt > 1:3105947/1‑69 (MQ=255) tCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCt > 1:2561924/1‑69 (MQ=255) tCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAAACGTTTATAGGCCTCATGTGCCt > 1:3222766/1‑69 (MQ=255) tACTGCATCGGACCTGCGTCGTTACGCGCACCGCCa > 1:2856930/1‑36 (MQ=255) tACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGt > 1:704854/1‑69 (MQ=255) tACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGt > 1:1643883/1‑69 (MQ=255) tACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGt > 1:1351496/1‑69 (MQ=255) tACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGt > 1:2482370/1‑69 (MQ=255) aCTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTa < 1:938409/69‑1 (MQ=255) aCTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTa < 1:2991280/69‑1 (MQ=255) gCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCt < 1:1906194/69‑1 (MQ=255) gCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCt < 1:1959855/69‑1 (MQ=255) gCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCt < 1:2448448/69‑1 (MQ=255) gCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCt < 1:3187971/69‑1 (MQ=255) gCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCt < 1:842683/69‑1 (MQ=255) gCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCt < 1:1378434/69‑1 (MQ=255) gCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGACTCATGTGCCTTTTGTACCt < 1:3163465/69‑1 (MQ=255) gCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGACTCATGTGCCTTTTGTACCt < 1:3159344/69‑1 (MQ=255) ggACCTGCGTCGTTACGCGCCCCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGtt > 1:592701/1‑67 (MQ=255) ggACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGtt > 1:665245/1‑67 (MQ=255) ggACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGtt > 1:1522124/1‑67 (MQ=255) ggACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGtt > 1:2976323/1‑67 (MQ=255) | ACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTT > minE/1888894‑1889018 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |