| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,889,568 | C→T | 45.4% | A221T (GCA→ACA) | gudX ← | predicted glucarate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,889,568 | 0 | C | T | 45.4% | 11.6 / 51.8 | 66 | A221T (GCA→ACA) | gudX | predicted glucarate dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (17/19); new base T (3/27); total (20/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.20e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01 | |||||||||||
CAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAAT > minE/1889505‑1889624 | caAGCAGCCAGGCACCATTGGGATCAACGGTAATCCGCGCATCAGGGAAGCGTTTCTTCAATGTACg > 1:579991/1‑67 (MQ=255)caAGCAGCCAGGCACCATTGGGATCAACGGTAATCCGCGCATCAGGGAAGCGTTTCTTCAATGTACg > 1:429695/1‑67 (MQ=255)caAGCAGCCAGGCACCATTGGGATCAACGGTAATCCGCGCATCAGGGAAGCGTTTCTTCAATGTACg > 1:1791840/1‑67 (MQ=255) cATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCg > 1:3260298/1‑69 (MQ=255) cATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCg > 1:597277/1‑69 (MQ=255) cATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCg > 1:1194342/1‑69 (MQ=255) caacGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGAtt > 1:3184319/4‑68 (MQ=255) cACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGAttt > 1:2179592/1‑69 (MQ=255) cACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGAtt > 1:2308972/1‑68 (MQ=255) cACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGAtt > 1:155519/1‑68 (MQ=255) cACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGAtt > 1:1529273/1‑68 (MQ=255) cACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGAtt > 1:342442/1‑68 (MQ=255) cACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGAtt > 1:1093408/1‑68 (MQ=255) gTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTg < 1:1495338/69‑1 (MQ=255) gTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTg < 1:877464/69‑1 (MQ=255) gTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTg < 1:3162330/69‑1 (MQ=255) gTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTg < 1:3199968/69‑1 (MQ=255) gTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTg < 1:3240810/69‑1 (MQ=255) gTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTg < 1:842061/69‑1 (MQ=255) gTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTg < 1:2289798/69‑1 (MQ=255) gTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTg < 1:1181048/69‑1 (MQ=255) gTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGCCGATTTCTTg < 1:1124002/69‑1 (MQ=255) ttGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTg < 1:2444231/68‑1 (MQ=255) aaCGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGc > 1:2179026/1‑65 (MQ=255) ggTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTaa < 1:1528404/69‑1 (MQ=255) aaTCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAacac > 1:3083030/1‑69 (MQ=255) aaTCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAacac > 1:1838265/1‑69 (MQ=255) aaTCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAacac > 1:2218321/1‑69 (MQ=255) cgcgCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACAcgccg > 1:1945194/1‑69 (MQ=255) cgcgCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACAcgccg > 1:2027901/1‑69 (MQ=255) cgcgCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACAcgcc > 1:521059/1‑68 (MQ=255) cGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAg < 1:3365871/69‑1 (MQ=255) cGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAg < 1:1516/69‑1 (MQ=255) aGCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGa < 1:546929/69‑1 (MQ=255) aGCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGa < 1:1014941/69‑1 (MQ=255) aGCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGa < 1:1265817/69‑1 (MQ=255) aGCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGa < 1:988925/69‑1 (MQ=255) aGCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGa < 1:1690191/69‑1 (MQ=255) aGCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGa < 1:2962003/69‑1 (MQ=255) aGCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGa < 1:3426864/69‑1 (MQ=255) aGCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGa < 1:2746502/69‑1 (MQ=255) aGCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGa < 1:1851409/69‑1 (MQ=255) aGCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGa < 1:2680508/69‑1 (MQ=255) gCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGaa < 1:867925/69‑1 (MQ=255) gCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGaa < 1:455526/69‑1 (MQ=255) gCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGaa < 1:1002739/69‑1 (MQ=255) gCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGaa < 1:75562/69‑1 (MQ=255) gCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGaa < 1:559868/69‑1 (MQ=255) gCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGaa < 1:992880/69‑1 (MQ=255) gCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGaa < 1:1346495/69‑1 (MQ=255) gCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGaa < 1:1496985/69‑1 (MQ=255) gCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGaa < 1:2626143/69‑1 (MQ=255) gCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGaa < 1:2476771/69‑1 (MQ=255) gCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGaa < 1:2274283/69‑1 (MQ=255) gCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGaa < 1:1590900/69‑1 (MQ=255) gCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGaa < 1:167651/69‑1 (MQ=255) gCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGaa < 1:1912205/69‑1 (MQ=255) gCGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGa < 1:2880919/68‑1 (MQ=255) cGTTTCTTCAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGaa < 1:3383804/68‑1 (MQ=255) gTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAAt < 1:2254041/69‑1 (MQ=255) gTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAAt < 1:96105/69‑1 (MQ=255) gTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAAt < 1:1986301/69‑1 (MQ=255) gTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAAt < 1:2547050/69‑1 (MQ=255) gTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAAt < 1:2693399/69‑1 (MQ=255) gTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAAt < 1:3173458/69‑1 (MQ=255) tcttcAATGTACGGACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCACCCTTAAGTTTGaa < 1:216596/64‑1 (MQ=255) | CAAGCAGCCATGCACCGTTGGGATCAACGGTAATCCGCGCATCCGGGAAGCGTTTCTTCAATGCACGAACAGTGTCGATTTCTTGCTCGCCAGGTAACACGCCGCCCTTAAGTTTGAAAT > minE/1889505‑1889624 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |