| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,891,013 | C→G | 48.8% | G190G (GGG→GGC) | gudP ← | predicted D‑glucarate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,891,013 | 0 | C | G | 48.8% | ‑3.3 / 48.6 | 41 | G190G (GGG→GGC) | gudP | predicted D‑glucarate transporter |
| Reads supporting (aligned to +/- strand): ref base C (3/18); new base G (8/12); total (11/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.55e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01 | |||||||||||
TCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCACTTCATGCGTCAGCCAGCCCAT > minE/1890946‑1891075 | tcttATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATc > 1:3058438/1‑68 (MQ=255)tcttATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATc > 1:1704877/1‑68 (MQ=255)tcttATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATc > 1:1552494/1‑68 (MQ=255) cttATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATc > 1:440281/1‑67 (MQ=255) tttGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAaagaagaa < 1:1009383/68‑1 (MQ=255) tttGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAaagaagaa < 1:102877/68‑1 (MQ=255) ttGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAagaagaaga < 1:62096/69‑1 (MQ=255) ttGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAagaagaaga < 1:2187123/69‑1 (MQ=255) cGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGtgtg < 1:1421868/69‑1 (MQ=255) cGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGtgtg < 1:1845398/69‑1 (MQ=255) gTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGtgtg < 1:3240597/68‑1 (MQ=255) gATGAGTTTCAACCAGATAAAGCTGATGACAATGCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCa < 1:557274/69‑1 (MQ=255) gATGACTTTCAACGAGATAAAGCTGATGACAATGCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCa < 1:1117007/69‑1 (MQ=255) gATGACTTTCAACCAGATAAAGCTGATGACAATGCCCAGACCGCCCATAAAGAAGAAGACGTTTGACCa < 1:435499/69‑1 (MQ=255) gATGACTTTCAACCAGATAAAGCTGATGACAATGCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCa < 1:523452/69‑1 (MQ=255) gATGACTTTCAACCAGATAAAGCTGATGACAATGCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCa < 1:854415/69‑1 (MQ=255) gATGACTTTCAACCAGATAAAGCTGATGACAATGCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCa < 1:1013876/69‑1 (MQ=255) gATGACTTTCAACCAGATAAAGCTGATGACAATGCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCa < 1:2897193/69‑1 (MQ=255) gATGACTTTCAACCAGATAAAGCTGATGACAATGCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCa < 1:2793343/69‑1 (MQ=255) gATGACTTTCAACCAGATAAAGCTGATGACAATGCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCa < 1:2724284/69‑1 (MQ=255) gATGACTTTCAACCAGATAAAGCTGATGACAATGCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCa < 1:1022307/69‑1 (MQ=255) gATGACTTTCAACCAGATAAAGCTGATGACAATGCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCa < 1:2031156/69‑1 (MQ=255) gATGACTTTCAACCAGATAAAGCTGATGACAATGCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCa < 1:1817124/69‑1 (MQ=255) caTGACTTTCAACCAGATAAAGCTGATGACAATCACCAGACCGCCCATAAAGAAGAAGACGTGTGACCa > 1:2252597/2‑69 (MQ=255) gACTTTCAACCAGATAAAGCTGATGACAATGCCCAGACCGc > 1:3232942/1‑41 (MQ=255) gACTTTCAACCAGATAAAGCTGATGACAATGCCCAGACCGc > 1:1075274/1‑41 (MQ=255) gACTTTCAACCAGATAAAGCTGATGACAATGCCCAGACCGc > 1:1290484/1‑41 (MQ=255) gACTTTCAACCAGATAAAGCTGATGACAATGCCCAGACCGc > 1:1548651/1‑41 (MQ=255) gACTTTCAACCAGATAAAGCTGATGACAATGCCCAGACCGc > 1:1749472/1‑41 (MQ=255) gACTTTCAACCAGATAAAGCTGATGACAATGCCCAGACCGc > 1:2984137/1‑41 (MQ=255) gACTTTCAACCAGATAAAGCTGATGACAATGCCCAGACCGc > 1:1827106/1‑41 (MQ=255) gACTTTCAACCAGATAAAGCTGATGACAATGCCCAGACCGc > 1:2769517/1‑41 (MQ=255) aaCCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCACtt < 1:2807955/67‑1 (MQ=255) aaCCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCACtt < 1:2430998/67‑1 (MQ=255) cAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCACTTCATGc > 1:3270850/1‑69 (MQ=255) cAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCACTTCATGc > 1:2316168/1‑69 (MQ=255) aaGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCACTTCATGCGTcagc < 1:2931854/69‑1 (MQ=255) aaGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCACTTCATGCGTcagc < 1:3321614/69‑1 (MQ=255) aaGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCACTTCATGCGTcagc < 1:3399359/69‑1 (MQ=255) aaGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCACTTCATGCGTcagc < 1:2213381/69‑1 (MQ=255) aaGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCACTTCATGCGTcagc < 1:487622/69‑1 (MQ=255) aaGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCACTTCATGCGTcagc < 1:748118/69‑1 (MQ=255) aaGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCACTTCATGCGTcagc < 1:2257770/69‑1 (MQ=255) gaCAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCACTTCATGCGTCAGCCAGCCCAt < 1:1624076/69‑1 (MQ=255) aCAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCACTTCATGCGTCAGCCAGCCCAt < 1:1373675/68‑1 (MQ=255) | TCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAGAAGAAGACGTGTGACCAGCCCACTTCATGCGTCAGCCAGCCCAT > minE/1890946‑1891075 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |