| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,891,538 | T→C | 40.8% | T15T (ACA→ACG) | gudP ← | predicted D‑glucarate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,891,538 | 0 | T | C | 40.8% | 35.4 / 67.1 | 93 | T15T (ACA→ACG) | gudP | predicted D‑glucarate transporter |
| Reads supporting (aligned to +/- strand): ref base T (45/10); new base C (10/28); total (55/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.11e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTGAAGGATGTGACGATAAACAACATCACCACTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTGAAAA > minE/1891487‑1891599 | gTTGAAGGATGTGACGATAAACAACATCACCACTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACt > 1:2481553/1‑69 (MQ=255)gTTGAAGGATGTGACGATAAACAACATCACCACTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACt > 1:2013314/1‑69 (MQ=255)gTTGAAGGATGTGACGATAAACAACATCACCACTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACt > 1:187996/1‑69 (MQ=255)gTTGAAGGATGTGACGATAAACAACATCACCACTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACt > 1:1821374/1‑69 (MQ=255)gTTGAAGGATGTGACGATAAACAACATCACCACTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACt > 1:506275/1‑69 (MQ=255)gTTGAAGGATGTGACGATAAACAACATCACCACTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACt > 1:2141515/1‑69 (MQ=255)gTTGAAGGATGTGACGATAAACAACATCACCACTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACt > 1:2684778/1‑69 (MQ=255)gTTGAAGGATGTGACGATAAACAACATCACCACTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACt > 1:289318/1‑69 (MQ=255)gTTGAAGGATGTGACGATAAACAACATCACCACTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACt > 1:1026455/1‑69 (MQ=255)gTTGAAGGATGTGACGATAAACAACATCACCACTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACt > 1:3054099/1‑69 (MQ=255) tgACGATAAACAACATCACCACTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCt > 1:1126065/1‑69 (MQ=255) tgACGATAAACAACATCACCACTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCt > 1:1486367/1‑69 (MQ=255) tAAACAACATCACCACTATCCAGTAACTAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:2199803/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:377242/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:2963826/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:3110697/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:2045571/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:3085249/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:2059180/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:2082149/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:325877/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:413436/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:2704330/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:3278719/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:2976523/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:2812695/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:231981/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:3345793/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:3186608/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:2745162/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:935645/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:831398/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:749913/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:966143/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:112680/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:1634925/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:1687832/69‑1 (MQ=255) tAAACAACATCACCACTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:1966109/69‑1 (MQ=255) tCACCACTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCa > 1:1584833/1‑69 (MQ=255) caccacTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAt < 1:1647076/69‑1 (MQ=255) caccacTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAt < 1:1347576/69‑1 (MQ=255) caccacTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAt < 1:2256248/69‑1 (MQ=255) caccacTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAt < 1:2635491/69‑1 (MQ=255) caccacTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAt < 1:567023/69‑1 (MQ=255) accacTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAtt > 1:2960771/1‑69 (MQ=255) accacTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAtt > 1:1018276/1‑69 (MQ=255) accacTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAtt > 1:1192988/1‑69 (MQ=255) accacTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAtt > 1:2000346/1‑69 (MQ=255) accacTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAtt > 1:251764/1‑69 (MQ=255) accacTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAtt > 1:2492623/1‑69 (MQ=255) accacTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAtt > 1:2365843/1‑69 (MQ=255) accacTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAtt > 1:2340724/1‑69 (MQ=255) accacTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAtt > 1:1056409/1‑69 (MQ=255) accacTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAtt > 1:3425250/1‑69 (MQ=255) accacTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAtt > 1:3436194/1‑69 (MQ=255) accacTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAtt > 1:663509/1‑69 (MQ=255) accacTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAtt > 1:1817630/1‑69 (MQ=255) accacTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAt < 1:1375665/68‑1 (MQ=255) accacTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAt < 1:1229165/68‑1 (MQ=255) accacTATCCAGTAACGAGCATTTGTGCGTTTTACcac > 1:2931710/1‑38 (MQ=255) cTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAttgttg < 1:3175142/69‑1 (MQ=255) cTATCCAGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:2624348/54‑1 (MQ=255) tCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAttgt < 1:1333742/64‑1 (MQ=255) tCCAGTAACGAGCATTTGTGAGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAttgt < 1:1178660/64‑1 (MQ=255) cAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTc > 1:2545031/1‑69 (MQ=255) cAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTc > 1:1185933/1‑69 (MQ=255) cAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTc > 1:646739/1‑69 (MQ=255) aGTAACGAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTa < 1:975184/48‑1 (MQ=255) aaCGCGCATTTGTGCGTTTTTCCTCACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTg > 1:2934679/1‑68 (MQ=255) aaCGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTg > 1:3345188/1‑68 (MQ=255) aaCGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTg > 1:681128/1‑68 (MQ=255) aaCGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTg > 1:2474863/1‑68 (MQ=255) aaCGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTg > 1:3033334/1‑68 (MQ=255) aaCGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTg > 1:2690632/1‑68 (MQ=255) aaCGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTg > 1:2442989/1‑68 (MQ=255) aaCGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTg > 1:1050264/1‑68 (MQ=255) aaCGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTg > 1:2252991/1‑68 (MQ=255) aaCGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTg > 1:2153281/1‑68 (MQ=255) aaCGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTg > 1:2121477/1‑68 (MQ=255) aaCGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTg > 1:2120142/1‑68 (MQ=255) aaCGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTg > 1:211172/1‑68 (MQ=255) aaCGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTg > 1:1836943/1‑68 (MQ=255) aaCGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTg > 1:1250409/1‑68 (MQ=255) gAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGc > 1:3253065/1‑58 (MQ=255) gAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTGaaaa > 1:994793/1‑69 (MQ=255) gAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTGaaaa > 1:1100954/1‑69 (MQ=255) gAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTGaaaa > 1:1414858/1‑69 (MQ=255) gAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTGaaaa > 1:146776/1‑69 (MQ=255) gAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTGaaaa > 1:697487/1‑69 (MQ=255) gAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTGaaaa > 1:1593275/1‑69 (MQ=255) gAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTGaaaa > 1:397523/1‑69 (MQ=255) gAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTGaaaa > 1:3375/1‑69 (MQ=255) gAGCATTCGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTGaaaa > 1:3082169/1‑69 (MQ=255) tgtgCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAttgttg < 1:1469007/50‑1 (MQ=255) tgtgCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCAttgttg < 1:1614287/50‑1 (MQ=255) | GTTGAAGGATGTGACGATAAACAACATCACCACTATCCAGTAACGAGCATTTGTGCGTTTTTCCACACTGCTCGCAGCCTGACTTAAAGAACTCATTGTTGCACTCCTGAAAA > minE/1891487‑1891599 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |