| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,892,400 | A→G | 100% | D160D (GAT→GAC) | ygdL ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,892,400 | 0 | A | G | 79.2% | 58.9 / 8.2 | 43 | D160D (GAT→GAC) | ygdL | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (5/4); new base G (34/0); total (39/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.02e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGCCAGCGGGTCCTGAATCGTTTTCGCCAGATCGGTAACCTGAATCTGCGTCGGATCAATCTGCCCACCCGCGCCACCGGTTGTGACCAGTGGGATTTTATTGCGCCGACAATAAGCAATC > minE/1892346‑1892466 | cgccAGCGGGTCCTGAATCGTTTTCGCCAGATCGGTAACCTGAATCTGCGTCGGATCAATCTGCCCAcc < 1:700683/69‑1 (MQ=255) ccAACGGGTCCTGCATCGTTTTCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCAc > 1:1362180/1‑66 (MQ=255) ccAACGGGTCCTGAATCGTTTTCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCAccag > 1:2055500/1‑67 (MQ=255) ccAACGGGTCCTGAATCGTTTTCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCAccag > 1:208525/1‑67 (MQ=255) ccAACGGGTCCTGAATCGTTTTCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCAccag > 1:2032930/1‑67 (MQ=255) ccAACGGGTCCTGAATCGTTTTCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCAccag > 1:1921358/1‑67 (MQ=255) ccAACGGGTCCTGAATCGTTTTCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCAccag > 1:1906537/1‑67 (MQ=255) ccAACGGGTCCTGAATCGTTTTCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCAccag > 1:1888725/1‑67 (MQ=255) ccAACGGGTCCTGAATCGTTTTCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCAccag > 1:2094078/1‑67 (MQ=255) ccAACGGGTCCTGAATCGTTTTCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCAccag > 1:2175618/1‑67 (MQ=255) ccAACGGGTCCTGAATCGTTTTCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCAccag > 1:2615714/1‑67 (MQ=255) ccAACGGGTCCTGAATCGTTTTCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCAccag > 1:2837152/1‑67 (MQ=255) ccAACGGGTCCTGAATCGTTTTCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCAccag > 1:3277311/1‑67 (MQ=255) ccAACGGGTCCTGAATCGTTTTCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCAccag > 1:1524470/1‑67 (MQ=255) ccAACGGGTCCTGAATCGTTTTCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCAccag > 1:3278129/1‑67 (MQ=255) ccAACGGGTCCTGAATCGTTTTCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCAccag > 1:3305128/1‑67 (MQ=255) ccAACGGGTCCTGAATCGTTTTCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCAccag > 1:3348225/1‑67 (MQ=255) ccAACGGGTCCTGAATCGTTTTCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCAccag > 1:407750/1‑67 (MQ=255) ccAACGGGTCCTGAATCGTTTTCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCAccag > 1:1097778/1‑67 (MQ=255) ccAACGGGTCCTGAATCGTTTTCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCAcca > 1:1061644/1‑67 (MQ=255) ccAACGGGTCCTGAATCGTTTTCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCAcca > 1:1347491/1‑67 (MQ=255) ccAACGGGTCCTGAATCGTTTTCGCCAGATAGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCAccag > 1:2552799/1‑67 (MQ=255) ttCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCACCAGCGCCACCg > 1:1721078/1‑57 (MQ=255) ttCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCACCAGCGCCACCGGtc > 1:2660341/1‑59 (MQ=255) ttCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCACCAGCGCCACCGGtc > 1:33977/1‑59 (MQ=255) ttCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCACCAGCGCCACCGGtc > 1:429299/1‑59 (MQ=255) ttCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCACCAGCGCCACCGGtc > 1:2619361/1‑59 (MQ=255) ttCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCACCAGCGCCACCGGtc > 1:1650846/1‑59 (MQ=255) ttCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCACCAGCGCCACCGGtc > 1:2516616/1‑59 (MQ=255) ttCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCACCAGCGCCACCGGTCGTGACCAgc > 1:1018029/1‑68 (MQ=255) ttCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCACCAGCGCCACCGGTCGTGACCAgc > 1:3153869/1‑68 (MQ=255) ttCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCACCAGCGCCACCGGTCGTGACCAgc > 1:539401/1‑68 (MQ=255) ttCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCACCAGCGCCACCGGTCGTGACCAgc > 1:1455811/1‑68 (MQ=255) ttCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATCTGCCCACCAGCGCCACCGGTCGTGACCAgc > 1:2173373/1‑68 (MQ=255) ttCGCCAGATCGGTAACCTGAATCTGCGTCGGGTCAATATGCCCACCAGCGCCACCGGtc > 1:3052363/1‑59 (MQ=255) tGAATCTGCGTCGGATCAATCTGCCCACCCGCGCCACCGGTTGTGAcc > 1:2327208/1‑48 (MQ=255) tGAATCTGCGTCGGATCAATCTGCCCACCCGCGCCACCGGTTGTGACCAGTGGGATTTTATTGCGCCg > 1:966495/1‑68 (MQ=255) tGAATCTGCGTCGGATCAATCTGCCCACCCGCGCCACCGGTTGTGACCAGTGGGATTTTATTGCGCCg > 1:2034413/1‑68 (MQ=255) tGAATCTGCGTCGGATCAATCTGCCCACCCGCGCCACCGGTTGTGACCAGTGGGATTTTATTGCGCCg > 1:1229570/1‑68 (MQ=255) tGAATCTGCGTCGGATCAATCTGCCCACCCGCGCCACCGGTTGTGACCAGTGGGATTTTATTGCGCCg > 1:1793086/1‑68 (MQ=255) gAATCTGCGTCGGATCAATCTGCCCACCCGCGCCACCGGTTGCGACCAGTGGGATTTTATTGCGCCGAc < 1:15922/69‑1 (MQ=255) ggATCAATCTGCCCACCCGCGCCACCGGTTGTGACCAGTGGGATTTTATTGCGCCGACAATAAGCAATc < 1:1466977/69‑1 (MQ=255) ggATCAATCTGCCCACCCGCGCCACCGGTTGTGACCAGTGGGATTTTATTGCGCCGACAATAAGCAATc < 1:1645942/69‑1 (MQ=255) | CGCCAGCGGGTCCTGAATCGTTTTCGCCAGATCGGTAACCTGAATCTGCGTCGGATCAATCTGCCCACCCGCGCCACCGGTTGTGACCAGTGGGATTTTATTGCGCCGACAATAAGCAATC > minE/1892346‑1892466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |