| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,892,496 | G→A | 29.5% | A128A (GCC→GCT) | ygdL ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,892,496 | 0 | G | A | 29.5% | 99.3 / 44.0 | 98 | A128A (GCC→GCT) | ygdL | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (21/48); new base A (16/13); total (37/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.48e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.34e-01 | |||||||||||
GGATTTTATTGCGCCGACAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATAACCCACGCTCATATACTGCGCTACGTTATCCGGCGTCACGAAAT > minE/1892438‑1892560 | ggATTTTATTGCGCCGACAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCatcaatca > 1:3049113/1‑69 (MQ=255)ggATTTTATTGCGCCGACAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCatcaatca < 1:3202177/69‑1 (MQ=255) tttATTGCGCCGACAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCa > 1:3310092/1‑58 (MQ=255) ttATTGCGCCGACAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCatcaat < 1:1853276/62‑1 (MQ=255) gcgcCGACAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATa < 1:255645/69‑1 (MQ=255) gcgcCGACAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATa < 1:368440/69‑1 (MQ=255) gcgcCGACAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATa < 1:1509953/69‑1 (MQ=255) gcgcCGACAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATa < 1:348319/69‑1 (MQ=255) gcgcCGACAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATa < 1:2147865/69‑1 (MQ=255) gcgcCGACAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATa < 1:31953/69‑1 (MQ=255) gcgcCGACAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATa < 1:2509078/69‑1 (MQ=255) gcCGACAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATa < 1:3224312/67‑1 (MQ=255) aCAATAATCAATCAGCGCCGCTTTGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAAccggc < 1:289864/69‑4 (MQ=255) aCAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATAGCATCTATCACGTACGAATAAccggc < 1:734911/69‑4 (MQ=255) aCAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAAccggc < 1:1998173/69‑4 (MQ=255) aCAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAAccggc < 1:2148333/69‑4 (MQ=255) aCAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAAccggc < 1:1670868/69‑4 (MQ=255) aCAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAAccggc < 1:36720/69‑4 (MQ=255) aCAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAAccggc < 1:1637729/69‑4 (MQ=255) aCAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAAccggc < 1:240985/69‑4 (MQ=255) aCAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAAccggc < 1:525304/69‑4 (MQ=255) aCAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAAccggc < 1:844724/69‑4 (MQ=255) aCAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAAccggc < 1:2249903/69‑4 (MQ=255) aCAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAAccggc < 1:1433836/69‑4 (MQ=255) aCAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAAccggc < 1:1399031/69‑4 (MQ=255) aTAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGGATCAATCACGTACGAATAACCCACGCt < 1:2236747/69‑1 (MQ=255) aaGCAATCAGCGCCGCTTTGGGCCGTACACTATCTATGGCATCAATCACGTACGAATAACCCACGCTCa < 1:573193/69‑1 (MQ=255) aaGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATGTCATCAATCACGTACGAATAACCCACGCTCa < 1:1758045/69‑1 (MQ=255) aaGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATAACCCACGCTCa < 1:2104891/69‑1 (MQ=255) aaGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATAACCCACGCTCa < 1:1248598/69‑1 (MQ=255) aaGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATAACCCACGCTCa < 1:659626/69‑1 (MQ=255) aaGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATAACCCACGCTCa < 1:1768013/69‑1 (MQ=255) aaGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATAACCCACGCTCa < 1:655626/69‑1 (MQ=255) aaGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATAACCCACGCTCa < 1:1678993/69‑1 (MQ=255) cAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATAACCCACGCTCata < 1:2343568/68‑1 (MQ=255) cAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATAACCCACGCTCata < 1:2968900/68‑1 (MQ=255) cAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATAACCCACGCTCata < 1:300052/68‑1 (MQ=255) cAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATAACCCACGCTCata < 1:259628/68‑1 (MQ=255) cAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATAACCCACGCTCata < 1:1965106/68‑1 (MQ=255) cAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATAACCCACGCTCata < 1:1612959/68‑1 (MQ=255) cAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATAACCCACGCTCata < 1:1374944/68‑1 (MQ=255) cAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATAACCCACGCTCata < 1:819886/68‑1 (MQ=255) cAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATAACCCACGCTCata < 1:870651/68‑1 (MQ=255) cAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATAACCCACGCTCata < 1:1018181/68‑1 (MQ=255) tCAGCGCCGCTTTGGGCCGTACACTATCAATAGCATCAATCACGTAc > 1:23748/1‑47 (MQ=255) tGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAACCGGCATTCATATATTGCGCTACGTTa > 1:903753/1‑68 (MQ=255) tGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAACCGGCATTCATATATTGCGCTACGTTAt > 1:356237/1‑69 (MQ=255) tGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAACCGGCATTCATATATTGCGCTACGTTAt > 1:304234/1‑69 (MQ=255) tGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAACCGGCATTCATATATTGCGCTACGTTAt > 1:3027132/1‑69 (MQ=255) tGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAACCGGCATTCATATATTGCGCTACGTTAt > 1:475825/1‑69 (MQ=255) tGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAACCGGCATTCATATATTGCGCTACGTTAt > 1:801414/1‑69 (MQ=255) tGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAACCGGCATTCATATATTGCGCTACGTTAt > 1:850571/1‑69 (MQ=255) tGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAACCGGCATTCATATATTGCGCTACGTTAt > 1:975794/1‑69 (MQ=255) tGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAACCGGCATTCATATATTGCGCTACGTTAt > 1:2656475/1‑69 (MQ=255) tGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAACCGGCATTCATATATTGCGCTACGTTAt > 1:1035403/1‑69 (MQ=255) tGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAACCGGCATTCATATATTGCGCTACGTTAt > 1:1118541/1‑69 (MQ=255) tGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAACCGGCATTCATATATTGCGCTACGTTAt > 1:1153281/1‑69 (MQ=255) tGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAACCGGCATTCATATATTGCGCTACGTTAt > 1:1660116/1‑69 (MQ=255) tGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAACCGGCATTCATATATTGCGCTACGTTAt > 1:1901931/1‑69 (MQ=255) tGGGCCGTACACTATCAATAGCATCAATCACGTACGAATAACCGGCATTAATATATTGCGCTAAGTTAt > 1:1656744/1‑69 (MQ=255) cacTATCAATGGCATGAATCACGTACGAATAACCCACGCTCata > 1:787527/1‑44 (MQ=255) cacTATCAATGGCATCAATCACGTACGAATAACCCACGCTCata > 1:2689596/1‑44 (MQ=255) cacTATCAATGGCATCAATCACGTACGAATAACCCACGCTCata > 1:1171644/1‑44 (MQ=255) cacTATCAATGGCATCAATCACGTACGAATAACCCACGCTCata > 1:1246676/1‑44 (MQ=255) cacTATCAATGGCATCAATCACGTACGAATAACCCACGCTCata > 1:750062/1‑44 (MQ=255) cacTATCAATGGCATCAATCACGTACGAATAACCCACGCTCata > 1:1594199/1‑44 (MQ=255) cacTATCAATGGCATCAATCACGTACGAATAACCCACGCTCata > 1:565897/1‑44 (MQ=255) cacTATCAATGGCATCAATCACGTACGAATAACCCACGCTCata > 1:1663474/1‑44 (MQ=255) cacTATCAATGGCATCAATCACGTACGAATAACCCACGCTCata > 1:3359827/1‑44 (MQ=255) cacTATCAATGGCATCAATCACGTACGAATAACCCACGCTCata > 1:2796933/1‑44 (MQ=255) cacTATCAATGGCATCAATCACGTACGAATAACCCACGCTCata > 1:19986/1‑44 (MQ=255) cacTATCAATGGCATCAATCACGTACGAATAACCCACGCTCata > 1:2206933/1‑44 (MQ=255) cacTATCAATGGCATCAATCACGTACGAATAACCCACGCTCa > 1:902145/1‑42 (MQ=255) aTCAATGGCATCAATCACGTACGAATAACCCACGCTCATATACTGCGCTACGTTATCCGGCGTCACGaa > 1:3304409/1‑69 (MQ=255) aTCAATGGCATCAATCACGTACGAATAACCCACGCTCATATACTGCGCTACGTTATCCGGCGTCACGaa > 1:380620/1‑69 (MQ=255) aTCAATGGCATCAATCACGTACGAATAACCCACGCTCATATACTGCGCTACGTTATCCGGCGTCACGaa > 1:2207303/1‑69 (MQ=255) aTCAATGGCATCAATCACGTACGAATAACCCACGCTCATATACTGCGCTACGTTATCCGGCGTCACGaa > 1:1431338/1‑69 (MQ=255) aTCAATGGCATCAATCACGTACGAATAACCCACGCTCATATACTGCGCTACGTTATCCGGCGTCACGaa > 1:2196954/1‑69 (MQ=255) aTCAATGGCATCAATCACGTACGAATAACCCACGCTCATATACTGCGCTACGTTATCCGGCGTCACGaa > 1:1506033/1‑69 (MQ=255) tCAATGGCATCAATCACGTACGAATAACCCACGCt < 1:1828537/35‑1 (MQ=255) tCAATGGCATCAATCACGTACGAATAACCCACGCt < 1:2855868/35‑1 (MQ=255) tCAATGGCATCAATCACGTACGAATAACCCACGCt < 1:821252/35‑1 (MQ=255) tCAATGGCATCAATCACGTACGAATAACCCACGCt < 1:1254726/35‑1 (MQ=255) tCAATGGCATCAATCACGTACGAATAACCCACGCt < 1:1711110/35‑1 (MQ=255) aaTGGCATCAATCACGTACGAATAACCCACGCTCATATACTGCGCTACGTTATCCGGCGTCACGAAat < 1:657385/68‑1 (MQ=255) aaTGGCATCAATCACGTACGAATAACCCACGCTCATATACTGCGCTACGTTATCCGGCGTCACGAAat < 1:2096338/68‑1 (MQ=255) aaTGGCATCAATCACGTACGAATAACCCACGCTCATATACTGCGCTACGTTATCCGGCGTCACGAAat < 1:1351755/68‑1 (MQ=255) aaTGGCATCAATCACGTACGAATAACCCACGCTCATATACTGCGCTACGTTATCCGGCGTCACGAAat < 1:1617599/68‑1 (MQ=255) aaTGGCATCAATCACGTACGAATAACCCACGCTCATATACTGCGCTACGTTATCCGGCGTCACGAAat < 1:2001870/68‑1 (MQ=255) aaTGGCATCAATCACGTACGAATAACCCACGCTCATATACTGCGCTACGTTATCCGGCGTCACGAAat < 1:428738/68‑1 (MQ=255) aaTGGCATCAATCACGTACGAATAACCCACGCTCATATACTGCGCTACGTTATCCGGCGTCACGAAat < 1:2988556/68‑1 (MQ=255) aaTGGCATCAATCACGTACGAATAACCCACGCTCATATACTGCGCTACGTTATCCGGCGTCACGAAat < 1:407358/68‑1 (MQ=255) aaTGGCATCAATCACGTACGAATAACCCACGCTCATATACTGCGCTACGTTATCCGGCGTCACGAAat < 1:2405498/68‑1 (MQ=255) aaTGGCATCAATCACGTACGAATAACCCACGCTCATATACTGCGCTACGTTATCCGGCGTCACGAAat < 1:2521080/68‑1 (MQ=255) aaTGGCATCAATCACGTACGAATAACCCACGCTCATATACTGCGCTACGTTATCCGGCGTCACGAAat < 1:1128224/68‑1 (MQ=255) aaTGGCATCAATCACGTACGAATAACCCACGCTCATATACTGCGCTACGTTATCCGGCGTCACGAAat < 1:380881/68‑1 (MQ=255) aaTGGCATCAATCACGTACGAATAACCCACGCTCATATACTGCGCTACGTTATCCGGCGTCACGAAat < 1:920655/68‑1 (MQ=255) aaTGGCATCAATCACGTACGAATAACCCACGCTCATATACTGCGCTACGTTATCCGGCGTCACGAAat < 1:1896458/68‑1 (MQ=255) | GGATTTTATTGCGCCGACAATAAGCAATCAGCGCCGCTTTGGGCCGTACACTATCAATGGCATCAATCACGTACGAATAACCCACGCTCATATACTGCGCTACGTTATCCGGCGTCACGAAAT > minE/1892438‑1892560 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |