| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,893,820 | G→A | 18.7% | R132R (CGC→CGT) | mltA ← | membrane‑bound lytic murein transglycosylase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,893,820 | 0 | G | A | 18.7% | 80.5 / 9.0 | 53 | R132R (CGC→CGT) | mltA | membrane‑bound lytic murein transglycosylase A |
| Reads supporting (aligned to +/- strand): ref base G (30/13); new base A (3/7); total (33/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.01e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATA > minE/1893761‑1893879 | gaCGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATgg < 1:1075611/69‑1 (MQ=255)gaCGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATgg < 1:804421/69‑1 (MQ=255) aCGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATgg < 1:1479982/68‑1 (MQ=255) gCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATgg < 1:2650733/65‑1 (MQ=255) gCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATgg < 1:797756/65‑1 (MQ=255) cAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATgg < 1:1211785/64‑1 (MQ=255) gcggcATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCgtg < 1:2360531/68‑1 (MQ=255) gcggcATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCgtg < 1:1957127/68‑1 (MQ=255) ggcATACGGTAAATCGGATACTTGAACTCGCCCTGACGGGTATGGCGCGCCTGAATTACCGGCgtgt < 1:82965/67‑1 (MQ=255) ggcATACGGTAAATCGGATACTGGAACTCGCCCTGACGGGTATGGCGCGCCTGAATTACCGGCgtgt < 1:880593/67‑1 (MQ=255) ggcATACGGTAAATCGGATACTGGAACTCGCCCTGACGGGTATGGCGCGCCTGAATTACCGGCgtgt < 1:2656369/67‑1 (MQ=255) ggcATACGGTAAATCGGATACTGGAACTCGCCCTGACGGGTATGGCGCGCCTGAATTACCGGCgtgt < 1:2429606/67‑1 (MQ=255) ggcATACGGTAAATCGGATACTGGAACTCGCCCTGACGGGTATGGCGCGCCTGAATTACCGGCgtgt < 1:641543/67‑1 (MQ=255) ggcATACGGTAAATCGGATACTGGAACTCGCCCTGACGGGTATGGCGCGCCTGAATTACCGGCgtgt < 1:696677/67‑1 (MQ=255) ggcATACGGTAAATCGGATACTGGAACTCGCCCTGACGGGTATGGCGCGCCTGAATTACAGGCgtgt < 1:2000558/67‑1 (MQ=255) aaaTAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCgtgt > 1:1674699/1‑57 (MQ=255) aaaTAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCgtgt < 1:640809/57‑1 (MQ=255) aaaTAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTa > 1:2764166/1‑68 (MQ=255) aaaTAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTa > 1:2534312/1‑68 (MQ=255) aaaTAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTa > 1:1852046/1‑68 (MQ=255) aaaTAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTa > 1:2025315/1‑68 (MQ=255) aaaTAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTa > 1:2043328/1‑68 (MQ=255) aaaTAGGATACTGGAACTCCCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCgtgt < 1:1057793/57‑1 (MQ=255) aaTAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGcc > 1:554288/1‑40 (MQ=255) aaTAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTg > 1:1692877/1‑42 (MQ=255) aaTAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTg > 1:2936152/1‑42 (MQ=255) aaTAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTg > 1:1172879/1‑42 (MQ=255) aaTAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTg > 1:2342482/1‑42 (MQ=255) aaTAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTg > 1:2281342/1‑42 (MQ=255) aaTAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTg > 1:2984449/1‑42 (MQ=255) aaTAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTg > 1:2050350/1‑42 (MQ=255) aaTAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTg > 1:40744/1‑42 (MQ=255) aaTAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTg < 1:520124/42‑1 (MQ=255) aaTAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTg > 1:1729728/1‑42 (MQ=255) aaTAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCgtgt < 1:1037844/56‑1 (MQ=255) ggATACTGGAACTCGCCCTTACGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAGCCTGTAAACTGc > 1:2734274/1‑69 (MQ=255) ggATACTGGAACTCGCCCTGACGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAGCCTGTAAACTGc > 1:2575965/1‑69 (MQ=255) ggATACTGGAACTCGCCCTGACGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAGCCTGTAAACTGc > 1:1785175/1‑69 (MQ=255) aCTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGt > 1:2245052/1‑69 (MQ=255) cTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAAc > 1:121105/1‑61 (MQ=255) cTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGtt > 1:891671/1‑69 (MQ=255) cTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGtt > 1:1006853/1‑69 (MQ=255) cTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGtt > 1:1094074/1‑69 (MQ=255) cTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGtt > 1:819391/1‑69 (MQ=255) cTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGtt > 1:1121640/1‑69 (MQ=255) cTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGtt > 1:1494682/1‑69 (MQ=255) cTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGtt > 1:1644289/1‑69 (MQ=255) cTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGtt > 1:1776822/1‑69 (MQ=255) cTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGtt > 1:180677/1‑69 (MQ=255) cTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGtt > 1:1806833/1‑69 (MQ=255) cTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGtt > 1:2457449/1‑69 (MQ=255) cTGGAACTAGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGtt > 1:3346090/1‑69 (MQ=255) cTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATa < 1:3060947/69‑1 (MQ=255) | GACGGCAGACGACCACGTTTTGGCGGCATACGGTAAATAGGATACTGGAACTCGCCCTGGCGGGTATGGCGCGCCTGAATTACCGGCGTGTAATAACCGGTAAACTGCACGTTACCATA > minE/1893761‑1893879 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |