| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,896,246 | C→A | 40.9% | intergenic (‑199/‑33) | amiC ← / → argA | N‑acetylmuramoyl‑L‑alanine amidase/fused acetylglutamate kinase homolog (inactive) and amino acid N‑acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,896,246 | 0 | C | A | 40.9% | 59.2 / 76.3 | 88 | intergenic (‑199/‑33) | amiC/argA | N‑acetylmuramoyl‑L‑alanine amidase/fused acetylglutamate kinase homolog (inactive) and amino acid N‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (6/46); new base A (23/13); total (29/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.37e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
TAAAAAAGTCGCTCTCGCATAAAATTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAGGTGTGCCGTGGTAAAGGAACGTAAAACCGAGTTGGTCGAGGGA > minE/1896182‑1896314 | tAAAAAAGTCGCTCTCGCATAAAATTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAAt > 1:2193017/1‑69 (MQ=255)tAAAAAAGTCGCTCTCGCATAAAATTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAAt > 1:237518/1‑69 (MQ=255)tAAAAAAGTCGCTCTCGCATAAAATTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAAt > 1:7169/1‑69 (MQ=255) aTAAAATTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCaa < 1:1138380/68‑1 (MQ=255) aTAAAATTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCaa < 1:870644/68‑1 (MQ=255) aaaTTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAagag > 1:3341205/1‑69 (MQ=255) aTTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATTCACTAATTTCTAATAATCATGCAAAGAgg < 1:1112354/68‑1 (MQ=255) aTTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCTAAGAgg < 1:5281/68‑1 (MQ=255) aTTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:367152/68‑1 (MQ=255) aTTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:757939/68‑1 (MQ=255) aTTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:2190070/68‑1 (MQ=255) aTTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:78814/68‑1 (MQ=255) aTTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:1762718/68‑1 (MQ=255) aTTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:79125/68‑1 (MQ=255) aTTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:688005/68‑1 (MQ=255) aTTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:573009/68‑1 (MQ=255) aTTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:3132045/68‑1 (MQ=255) aTTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:3428202/68‑1 (MQ=255) aTTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:3398922/68‑1 (MQ=255) aTTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCCAATAATCATGCAAAGAgg < 1:588293/68‑1 (MQ=255) tttACACTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:1594776/67‑1 (MQ=255) acTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAGGTGTGcc < 1:2017986/68‑1 (MQ=255) acTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAGGTGTGcc < 1:185149/68‑1 (MQ=255) acTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAGGTGTGcc < 1:422390/68‑1 (MQ=255) acTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAGGTGTGcc < 1:901229/68‑1 (MQ=255) acTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAGGTGTGcc < 1:729464/68‑1 (MQ=255) cTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAGGTGTGcc < 1:2951470/67‑1 (MQ=255) tGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:3225083/59‑1 (MQ=255) tGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:2287565/59‑1 (MQ=255) tGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:2332967/59‑1 (MQ=255) tGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:2083175/59‑1 (MQ=255) tGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:2141384/59‑1 (MQ=255) tGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:828250/59‑1 (MQ=255) tGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:1741417/59‑1 (MQ=255) tGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:1129619/59‑1 (MQ=255) tGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:1588617/59‑1 (MQ=255) tGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:870238/59‑1 (MQ=255) tGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:1395572/59‑1 (MQ=255) tGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAGGTGTGCCGTg > 1:2623022/1‑68 (MQ=255) gCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAgg < 1:2637921/58‑1 (MQ=255) cTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAGGTGTGCCGTGGTAAAgg < 1:994668/69‑1 (MQ=255) cTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAGGTGTGCCGTGGTAAAgg < 1:1260932/69‑1 (MQ=255) tGCAAAAAAACAGAATAAAAATACAATAATTTCGaataat > 1:1588047/1‑40 (MQ=38) tGCAAAAAAACAGAATAAAAATACAATAATTTCGAATAATCATGGAAAGAGgtgtac > 1:1093319/1‑55 (MQ=255) tGCAAAAAAACAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAgg > 1:2880593/1‑68 (MQ=255) tGCAAAAAAACAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGa > 1:1382469/1‑69 (MQ=255) tGCAAAAAAACAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGa > 1:1376175/1‑69 (MQ=255) tGCAAAAAAACAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGa > 1:3150829/1‑69 (MQ=255) tGCAAAAAAACAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGa > 1:467111/1‑69 (MQ=255) tGCAAAAAAACAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGa > 1:1108904/1‑69 (MQ=255) tGCAAAAAAACAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGa > 1:1060929/1‑69 (MQ=255) tGCAAAAAAACAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGa > 1:2915688/1‑69 (MQ=255) tGCAAAAAAACAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGa > 1:1875264/1‑69 (MQ=255) tGCAAAAAAACAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGa > 1:2788222/1‑69 (MQ=255) tGCAAAAAAACAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGa > 1:1596636/1‑69 (MQ=255) tGCAAAAAAACAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGa > 1:1020622/1‑69 (MQ=255) tGCAAAAAAACAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGa > 1:1601468/1‑69 (MQ=255) tGCAAAAAAACAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGa > 1:2325587/1‑69 (MQ=255) tGCAAAAAAACAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGa > 1:814523/1‑69 (MQ=255) tGCAAAAAAACAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGa > 1:1658093/1‑69 (MQ=255) tGCAAAAAAACAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGa > 1:1818777/1‑69 (MQ=255) tGCAAAAAAACAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGa > 1:2179720/1‑69 (MQ=255) tGCAAAAAAACAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGa > 1:1989445/1‑69 (MQ=255) tGCAAAAAAACAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGAAAAGGa > 1:2223957/1‑69 (MQ=255) tGCAAAAAAACAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGAAAAGGa > 1:2220071/1‑69 (MQ=255) aaaaaaCAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAATGAACGt < 1:2733689/69‑1 (MQ=255) aaaaaaCAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGt < 1:606795/69‑1 (MQ=255) aaaaaaCAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGt < 1:2372540/69‑1 (MQ=255) aaaaaaCAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGt < 1:103329/69‑1 (MQ=255) aaaaaaCAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGt < 1:1460670/69‑1 (MQ=255) aaaaaaCAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGt < 1:3050808/69‑1 (MQ=255) aaaaaaCAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGt < 1:2986707/69‑1 (MQ=255) aaaaaaCAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGt < 1:2981242/69‑1 (MQ=255) aaaaaaCAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGt < 1:2766286/69‑1 (MQ=255) aaaaaaCAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGt < 1:151664/69‑1 (MQ=255) aaaaaaCAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGt < 1:1695043/69‑1 (MQ=255) aaaaaaCAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGt < 1:2533620/69‑1 (MQ=255) aaaaaaCAGAATAAAAATACAATAATTTCGAATAATCATGCAAAGAGGTGTACCGTGGTAAAGGAACGt < 1:1977891/69‑1 (MQ=255) aaaaCAGAATAAAAATACACTAATTTCGAATAATCATGCa < 1:2267348/40‑1 (MQ=255) aaaaCAGAATAAAAATACACTAATTTCGAATAATCATGCa < 1:1790863/40‑1 (MQ=255) aaaaCAGAATAAAAATACACTAATTTCGAATAATCATGCa < 1:2569800/40‑1 (MQ=255) aaaaCAGAATAAAAATACACTAATTTCGAATAATCATGCa < 1:633245/40‑1 (MQ=255) aGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAGGTGTGCCGTGGTAAAGGAACGTAAAACCg < 1:1657568/69‑1 (MQ=255) aGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAGGTGTGCCGTGGTAAAGGAACGTAAAACCg < 1:123818/69‑1 (MQ=255) aGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAGGTGTGCCGTGGTAAAGGAACGTAAAACCg < 1:934953/69‑1 (MQ=255) aTAAAAATACACTAATTTCGAATAATCATGCAAAGAGGTGTGCCGTGGTAAAGGAACGTAAAACCGAGt < 1:3268196/69‑1 (MQ=255) aTAAAAATACACTAATTTCGAATAATCATGCAAAGAGGTGTGCCGTGGTAAAGGAACGTAAAACCGAGt < 1:1335489/69‑1 (MQ=255) aaaaTACACTAATTTCGAATAATCATGCAAAGAGGTGTGCCGTGGTAAAGGAACGTAAAACCGAGTTgg > 1:3192545/1‑69 (MQ=255) cTAATTTCGAATAATCATGCAAAGAGGTGTGCCGTGGTAAAGGAACGTAAAACCGAGTTGGTCGAGGGa < 1:444332/69‑1 (MQ=255) | TAAAAAAGTCGCTCTCGCATAAAATTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATAATCATGCAAAGAGGTGTGCCGTGGTAAAGGAACGTAAAACCGAGTTGGTCGAGGGA > minE/1896182‑1896314 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |