Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 139,741 G→A 31.8% D142D (GAC→GAT htrE ← predicted outer membrane usher protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE139,7410GA31.8% 63.2 / 34.3 72D142D (GAC→GAThtrEpredicted outer membrane usher protein
Reads supporting (aligned to +/- strand):  ref base G (19/30);  new base A (10/13);  total (29/43)
Fisher's exact test for biased strand distribution p-value = 7.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

ATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATC  >  minE/139675‑139806
                                                                  |                                                                 
aTAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACg                                                                   >  1:756191/1‑67 (MQ=255)
  aGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTATCGTCAt                                                               <  1:442784/69‑1 (MQ=255)
  aGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCAt                                                               <  1:2103968/69‑1 (MQ=255)
  aGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCAt                                                               <  1:467084/69‑1 (MQ=255)
   gTTTTGATAATTCTTCATAATCCATGCCTGTGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                                                              <  1:749256/69‑1 (MQ=255)
   gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                                                              <  1:1022105/69‑1 (MQ=255)
   gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                                                              <  1:1157484/69‑1 (MQ=255)
   gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                                                              <  1:3377166/69‑1 (MQ=255)
   gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                                                              <  1:205781/69‑1 (MQ=255)
   gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                                                              <  1:3185350/69‑1 (MQ=255)
   gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                                                              <  1:1444888/69‑1 (MQ=255)
   gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                                                              <  1:2508012/69‑1 (MQ=255)
   gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                                                              <  1:1542301/69‑1 (MQ=255)
   gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                                                              <  1:1607980/69‑1 (MQ=255)
   gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                                                              <  1:2716367/69‑1 (MQ=255)
   gTTTTGATAATTCATCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                                                              <  1:2322446/69‑1 (MQ=255)
    ttttGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                                                              <  1:2030272/68‑1 (MQ=255)
         atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                                                         >  1:3069268/2‑68 (MQ=255)
         atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                                                         >  1:2896663/2‑68 (MQ=255)
         atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                                                         >  1:3082853/2‑68 (MQ=255)
         atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                                                         >  1:2474370/2‑68 (MQ=255)
         atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                                                         >  1:558154/2‑68 (MQ=255)
         atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                                                         >  1:3187997/2‑68 (MQ=255)
         atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                                                         >  1:2063249/2‑68 (MQ=255)
         atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                                                         >  1:866857/2‑68 (MQ=255)
         atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                                                         >  1:2141195/2‑68 (MQ=255)
         atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                                                         >  1:2365083/2‑68 (MQ=255)
              ttttCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGaa                                                    <  1:1530033/68‑1 (MQ=255)
              ttttCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGaa                                                    <  1:2434839/68‑1 (MQ=255)
                      aCCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAggg                                           <  1:1014071/69‑1 (MQ=255)
                      aCCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAggg                                           <  1:559956/69‑1 (MQ=255)
                      aCCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAggg                                           <  1:1226880/69‑1 (MQ=255)
                      aCCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAggg                                           <  1:1713788/69‑1 (MQ=255)
                      aCCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAggg                                           <  1:1256283/69‑1 (MQ=255)
                      aCCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAggg                                           <  1:2028433/69‑1 (MQ=255)
                      aCCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAggg                                           <  1:1861666/69‑1 (MQ=255)
                      aCCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAggg                                           <  1:717558/69‑1 (MQ=255)
                           ggCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATa                                        >  1:2675347/1‑67 (MQ=255)
                           ggCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAAt                                      >  1:1825668/1‑69 (MQ=255)
                           ggCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAAt                                      >  1:1346114/1‑69 (MQ=255)
                             cTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAAttt                                    >  1:2467767/1‑69 (MQ=255)
                             cTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAAttt                                    >  1:1611192/1‑69 (MQ=255)
                             cTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAAttt                                    >  1:2601714/1‑69 (MQ=255)
                             cTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAAttt                                    >  1:2161066/1‑69 (MQ=255)
                             cTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAAttt                                    >  1:2977560/1‑69 (MQ=255)
                             cTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAAttt                                    >  1:274251/1‑69 (MQ=255)
                                 aGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTcc                                  >  1:1960478/1‑67 (MQ=255)
                                 aGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTcc                                  >  1:418844/1‑67 (MQ=255)
                                      cGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCaaa                            >  1:2720893/1‑68 (MQ=255)
                                              tCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGa                                              >  1:2915543/1‑42 (MQ=255)
                                              tCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGa                                              >  1:1837057/1‑42 (MQ=255)
                                              tCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGa                                              >  1:2769694/1‑42 (MQ=255)
                                              tCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGc                     <  1:2234409/67‑1 (MQ=255)
                                              tCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGc                     <  1:2666753/67‑1 (MQ=255)
                                              tCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGc                     <  1:1908415/67‑1 (MQ=255)
                                              tCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGc                     <  1:603641/67‑1 (MQ=255)
                                              tCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGc                     <  1:2394969/67‑1 (MQ=255)
                                              tCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGc                     <  1:1188330/67‑1 (MQ=255)
                                              tCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGc                     <  1:2289047/67‑1 (MQ=255)
                                              tCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCa                    <  1:2570652/68‑1 (MQ=255)
                                                        gATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCg         <  1:2426080/69‑1 (MQ=255)
                                                        gATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCg         >  1:315023/1‑69 (MQ=255)
                                                        gATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCg         <  1:222296/69‑1 (MQ=255)
                                                        gATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCg         <  1:1988688/69‑1 (MQ=255)
                                                        gATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCg         >  1:1537568/1‑69 (MQ=255)
                                                           ctttAACGTCATAACGAACAGAAGCCTTAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGtt       <  1:1306890/66‑1 (MQ=255)
                                                               aaCGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATc  <  1:384292/69‑1 (MQ=255)
                                                               aaCGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATc  <  1:32139/69‑1 (MQ=255)
                                                               aaCGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATc  >  1:552636/1‑69 (MQ=255)
                                                               aaCGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATc  <  1:246150/69‑1 (MQ=255)
                                                               aaCGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATc  <  1:121720/69‑1 (MQ=255)
                                                               aaCGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATc  <  1:1034305/69‑1 (MQ=255)
                                                               aaCGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATc  >  1:941436/1‑69 (MQ=255)
                                                                  |                                                                 
ATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAATTTCCGTCAAATTGAGGCAATTGCCGAGCGTTTCATC  >  minE/139675‑139806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: