| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,897,196 | T→C | 30.9% | I306I (ATT→ATC) | argA → | fused acetylglutamate kinase homolog (inactive) and amino acid N‑acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,897,196 | 0 | T | C | 30.9% | 116.9 / 64.8 | 129 | I306I (ATT→ATC) | argA | fused acetylglutamate kinase homolog (inactive) and amino acid N‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (18/71); new base C (13/27); total (31/98) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 129 total aligned reads displayed.
GGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCG > minE/1897131‑1897260 | tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg < 1:2597848/69‑1 (MQ=255) tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg < 1:1473/69‑1 (MQ=255) tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg < 1:1709628/69‑1 (MQ=255) tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg < 1:1732358/69‑1 (MQ=255) tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg < 1:1771095/69‑1 (MQ=255) tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg < 1:642859/69‑1 (MQ=255) tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg < 1:2149952/69‑1 (MQ=255) tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg < 1:2161967/69‑1 (MQ=255) tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg < 1:2637127/69‑1 (MQ=255) tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg < 1:361383/69‑1 (MQ=255) tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg < 1:2694467/69‑1 (MQ=255) tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg < 1:89039/69‑1 (MQ=255) tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg < 1:2734680/69‑1 (MQ=255) tATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCgg < 1:323133/69‑1 (MQ=255) tCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTa < 1:3351998/69‑1 (MQ=255) tCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTa < 1:1847057/69‑1 (MQ=255) tCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTa < 1:2770200/69‑1 (MQ=255) tCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTa < 1:2986510/69‑1 (MQ=255) ggTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTAt < 1:1216850/68‑1 (MQ=255) ggTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTAt < 1:2086323/68‑1 (MQ=255) ttGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGa < 1:1163911/69‑1 (MQ=255) ttGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGa < 1:1317320/69‑1 (MQ=255) ttGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGa < 1:2530197/69‑1 (MQ=255) ttGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGa > 1:1615385/1‑69 (MQ=255) ttGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGa < 1:877881/69‑1 (MQ=255) ttGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGa < 1:2875934/69‑1 (MQ=255) ttGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGa < 1:843938/69‑1 (MQ=255) ttGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGa < 1:2623358/69‑1 (MQ=255) ttGTGATGGAAAGCGCAGATCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGa < 1:1518041/69‑1 (MQ=255) tgtgATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGa < 1:1509382/68‑1 (MQ=255) gATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTc < 1:217243/68‑1 (MQ=255) gATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTc < 1:278774/68‑1 (MQ=255) gATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTc < 1:443149/68‑1 (MQ=255) gATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTc < 1:477743/68‑1 (MQ=255) gATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTc < 1:687927/68‑1 (MQ=255) gATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTc < 1:1065384/68‑1 (MQ=255) gATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTc < 1:1678972/68‑1 (MQ=255) gATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTc < 1:753104/68‑1 (MQ=255) gATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGAAGTTGATTc < 1:3421936/68‑1 (MQ=255) aTGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCg > 1:2119461/1‑68 (MQ=255) gAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCa > 1:800519/1‑69 (MQ=255) ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:2668550/69‑1 (MQ=255) ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:2763032/69‑1 (MQ=255) ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:1461671/69‑1 (MQ=255) ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:2206488/69‑1 (MQ=255) ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:862214/69‑1 (MQ=255) ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:818673/69‑1 (MQ=255) ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:1479170/69‑1 (MQ=255) ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:3184359/69‑1 (MQ=255) ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:1345687/69‑1 (MQ=255) ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:1347279/69‑1 (MQ=255) cGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:2259412/68‑1 (MQ=255) cGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:91300/68‑1 (MQ=255) gAGCAGATTCGTCGCGCAACAATCATCGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:2484970/67‑1 (MQ=255) gATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGa < 1:2063987/47‑1 (MQ=255) gATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAgg < 1:1873488/69‑1 (MQ=255) gATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAgg < 1:73782/69‑1 (MQ=255) gATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAgg < 1:351389/69‑1 (MQ=255) gATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAgg < 1:1200757/69‑1 (MQ=255) gATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAgg < 1:1245847/69‑1 (MQ=255) gATTCGTCGCGCAACAATCAACGATATTGGCGGAATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAgg < 1:3142079/69‑1 (MQ=255) ttCGTCGCGCAACAATCAAGGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGt > 1:2788910/1‑68 (MQ=255) ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa > 1:3378884/1‑69 (MQ=255) ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa > 1:558509/1‑69 (MQ=255) ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa < 1:824599/69‑1 (MQ=255) ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa > 1:3179443/1‑69 (MQ=255) ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa < 1:925718/69‑1 (MQ=255) ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa < 1:936442/69‑1 (MQ=255) ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa < 1:1767693/69‑1 (MQ=255) ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa < 1:1854208/69‑1 (MQ=255) ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa < 1:1190648/69‑1 (MQ=255) gcgcAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTgg < 1:2739634/69‑1 (MQ=255) aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt < 1:1453242/60‑1 (MQ=255) aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt < 1:1466533/60‑1 (MQ=255) aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt < 1:160171/60‑1 (MQ=255) aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt < 1:1451846/60‑1 (MQ=255) aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt < 1:3040000/60‑1 (MQ=255) aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt < 1:1221564/60‑1 (MQ=255) aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt < 1:1120087/60‑1 (MQ=255) aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt < 1:2847593/60‑1 (MQ=255) aTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGt < 1:115095/69‑1 (MQ=255) aTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGt < 1:2681626/69‑1 (MQ=255) aTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGt < 1:1603264/69‑1 (MQ=255) cGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTtctc > 1:275683/1‑68 (MQ=255) cGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTtctc > 1:3372361/1‑68 (MQ=255) cGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTtctc > 1:2463467/1‑68 (MQ=255) cGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTtctc > 1:3315917/1‑68 (MQ=255) cGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTtctc > 1:2938241/1‑68 (MQ=255) cGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTtct > 1:3368779/1‑67 (MQ=255) cGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTtct > 1:1029741/1‑67 (MQ=255) atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGt > 1:1718001/1‑55 (MQ=255) atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg > 1:1011712/1‑69 (MQ=255) atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg > 1:2076207/1‑69 (MQ=255) atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg > 1:675606/1‑69 (MQ=255) atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg > 1:2455724/1‑69 (MQ=255) atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg > 1:2773093/1‑69 (MQ=255) atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg > 1:3376157/1‑69 (MQ=255) atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg > 1:2790564/1‑69 (MQ=255) atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg > 1:2838834/1‑69 (MQ=255) atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg > 1:3038475/1‑69 (MQ=255) | GGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCG > minE/1897131‑1897260 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |