| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,901,226 | C→T | 24.4% | T605T (ACG→ACA) | recB ← | exonuclease V (RecBCD complex), beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,901,226 | 0 | C | T | 24.4% | 75.5 / 18.1 | 61 | T605T (ACG→ACA) | recB | exonuclease V (RecBCD complex), beta subunit |
| Reads supporting (aligned to +/- strand): ref base C (26/20); new base T (7/8); total (33/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01 | |||||||||||
GCGCGTTCAGCCCCATCATTGACGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAAC > minE/1901165‑1901282 | gcgcATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGtcatca < 1:487007/69‑1 (MQ=255)gcgcATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGtcatca < 1:2116892/69‑1 (MQ=255)gcgcATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGtcatca < 1:285945/69‑1 (MQ=255)gcgcATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGtcatca < 1:1965675/69‑1 (MQ=255)gcgcATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGtcatca < 1:3072191/69‑1 (MQ=255)gcgcATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGtcatca < 1:1562450/69‑1 (MQ=255)gcgcATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGtcatca < 1:2739752/69‑1 (MQ=255)gcgcATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACATTCGGGTGtcatca < 1:651576/69‑1 (MQ=255) cgcaTTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGtcatca > 1:2847672/1‑68 (MQ=255) cgcaTTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGTCATCAc > 1:932365/1‑69 (MQ=255) cgcaTTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGTCATCAc > 1:2146854/1‑69 (MQ=255) cgcaTTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGTCATCAc > 1:2513352/1‑69 (MQ=255) cgcaTTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGTCATCAc > 1:2815581/1‑69 (MQ=255) cgcaTTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGTCATCAc > 1:1557313/1‑69 (MQ=255) cgcaTTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGTCATCAc > 1:1552229/1‑69 (MQ=255) gcgTTCAGCCCCATCATTGACGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCAcc < 1:1155636/69‑1 (MQ=255) ttCAGCCCCATCATTGACGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGcc < 1:1779058/69‑1 (MQ=255) ttCAGCCCCATCATTGACGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGcc < 1:1863878/69‑1 (MQ=255) ttCAGCCCCATCATTGACGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGcc < 1:2027138/69‑1 (MQ=255) ttCAGCCCCATCATTGACGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGcc < 1:2869556/69‑1 (MQ=255) ttCAGCCCCATCATTGACGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGcc < 1:1276975/69‑1 (MQ=255) ttCAGCCCCATCATTGACGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGcc < 1:2483153/69‑1 (MQ=255) ttCAGCCCCATCATTGACGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGcc < 1:124085/69‑1 (MQ=255) ttCAGCCCCATCATTGACGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGcc < 1:3360472/69‑1 (MQ=255) cAGCCCCATCATTGACGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCt > 1:248281/1‑68 (MQ=255) cAGCCCCATCATTGACGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCt > 1:324432/1‑68 (MQ=255) cAGCCCCATCATTGACGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCt > 1:3065833/1‑68 (MQ=255) cAGCCCCATCATTGACGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCt > 1:2292200/1‑68 (MQ=255) cAGCCCCATCATTGACGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCt > 1:1590702/1‑68 (MQ=255) cAGCCCCATCATTGACGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCt > 1:4872/1‑68 (MQ=255) cAGCCCCATCATTGACGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCt > 1:2092940/1‑68 (MQ=255) cAGCCCCATCATTGACGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCt > 1:2046358/1‑68 (MQ=255) atTGACGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCa > 1:797897/1‑68 (MQ=255) cGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCa < 1:2343696/68‑1 (MQ=255) cGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCa < 1:760844/68‑1 (MQ=255) cGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCa < 1:679651/68‑1 (MQ=255) gCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTcc > 1:3077478/1‑69 (MQ=255) gCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTcc > 1:3221103/1‑69 (MQ=255) gCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTcc > 1:1672356/1‑69 (MQ=255) gCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTcc > 1:1538342/1‑69 (MQ=255) gCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTcc > 1:2169611/1‑69 (MQ=255) gCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTcc > 1:365893/1‑69 (MQ=255) gcACTACGCAGGGTGCTCTCACGTTCGGGCGTCATCACCGCCTGCAACACCCAAAGCATTTCCCGCGCt > 1:2143112/1‑69 (MQ=255) gcACTACGCAGGGTGCTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTgcgc > 1:1115169/1‑68 (MQ=255) gcACTACGCAGGGTGCTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCt > 1:1785831/1‑69 (MQ=255) gcACTACGCAGGGTGCTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCt > 1:2705822/1‑69 (MQ=255) gcACTACGCAGGGTGCTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACAAAAGCATTTCCTGCGCt > 1:2333187/1‑69 (MQ=255) cAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGt < 1:457684/69‑1 (MQ=255) cAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGt < 1:1042725/69‑1 (MQ=255) cAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGt < 1:2070216/69‑1 (MQ=255) cAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGt < 1:481187/69‑1 (MQ=255) cAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGt < 1:2366642/69‑1 (MQ=255) cAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGt < 1:2383787/69‑1 (MQ=255) cAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGt < 1:2406300/69‑1 (MQ=255) cAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGt < 1:2669856/69‑1 (MQ=255) ctcACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAac > 1:23581/1‑69 (MQ=255) ctcACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAac > 1:2117045/1‑69 (MQ=255) ctcACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAac > 1:3132590/1‑69 (MQ=255) ctcACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAac > 1:3126498/1‑69 (MQ=255) ctcACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAac > 1:1517966/1‑69 (MQ=255) ctcACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAac > 1:1341634/1‑69 (MQ=255) | GCGCGTTCAGCCCCATCATTGACGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACCGCCTGCAACAACCAAAGCATTTCCTGCGCTTCCAGAGTTTCAAAAAC > minE/1901165‑1901282 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |