| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 21,574 | C→T | 45.2% | V502V (GTC→GTT) | ileS → | isoleucyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 21,574 | 0 | C | T | 45.2% | 7.4 / 41.0 | 53 | V502V (GTC→GTT) | ileS | isoleucyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base C (10/19); new base T (9/15); total (19/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTA > minE/21506‑21639 | aaaGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTc < 1:2593601/69‑1 (MQ=255)aaaGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTc < 1:760468/69‑1 (MQ=255)aaaGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTc < 1:1441852/69‑1 (MQ=255)aaaGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTc < 1:2625284/69‑1 (MQ=255)aaaGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTc < 1:2905767/69‑1 (MQ=255)aaaGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTc < 1:3196549/69‑1 (MQ=255) aaGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTc < 1:1679322/68‑1 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCTTTGAAGTTGa > 1:551225/1‑69 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTGa > 1:2053686/1‑69 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTGa > 1:2390660/1‑69 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTGa > 1:2378297/1‑69 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTGa > 1:2645525/1‑69 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTGa > 1:2957925/1‑69 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTGa > 1:3340560/1‑69 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTGa > 1:2455100/1‑69 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTGa > 1:665643/1‑69 (MQ=255) tGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCgt < 1:3365331/69‑1 (MQ=255) tGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCgt < 1:745130/69‑1 (MQ=255) cgcgTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCa > 1:199037/1‑58 (MQ=255) cgcgTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGtggtgg > 1:332539/1‑68 (MQ=255) cgcgTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGtggtgg > 1:2382572/1‑68 (MQ=255) cgcgTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTggg < 1:3336667/69‑1 (MQ=255) cgcgTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTggg > 1:1371620/1‑69 (MQ=255) cgcgTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTggg < 1:1003257/69‑1 (MQ=255) aCCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGAtctc < 1:2542559/69‑1 (MQ=255) aCCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGAtctc < 1:886584/69‑1 (MQ=255) aaCTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGc > 1:3365479/1‑67 (MQ=255) aaCTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGc > 1:2612913/1‑67 (MQ=255) gATGGAAGAAGTGGCAAAACGCGTTGAAGTTGACGGCATTCAGGCGTGGTGTGATCTCGATGCGAAaga < 1:3314634/69‑1 (MQ=255) gATGGAAGAAGTGGCAAAACGCGTTGAAGTTGACGGCATTCAGGCGTGGTGGGATCTCGATGCGAAaga < 1:2510958/69‑1 (MQ=255) gATGGAAGAAGTGGCAAAACGCGTTGAAGTTGACGGCATTCAGGCGTGGTGGGATCTCGATGCGAAaga < 1:1317559/69‑1 (MQ=255) gATGGAAGAAGTGGCAAAACGCGTTGAAGTTGACGGCATTCAGGCGTGGTGGGATCTCGATGCGAAaga < 1:1340887/69‑1 (MQ=255) gATGGAAGAAGTGGCAAAACGCGTTGAAGTTGACGGCATTCAGGCGTGGTGGGATCTCGATGCGAAaga < 1:1454261/69‑1 (MQ=255) gATGGAAGAAGTGGCAAAACGCGTTGAAGTTGACGGCATTCAGGCGTGGTGGGATCTCGATGCGAAaga < 1:605578/69‑1 (MQ=255) gATGGAAGAAGTGGCAAAACGCGTTGAAGTTGACGGCATTCAGGCGTGGTGGGATCTCGATGCGAAaga < 1:1655350/69‑1 (MQ=255) gATGGAAGAAGTGGCAAAACGCGTTGAAGTTGACGGCATTCAGGCGTGGTGGGATCTCGATGCGAAaga < 1:1958253/69‑1 (MQ=255) gATGGAAGAAGTGGCAAAACGCGTTGAAGTTGACGGCATTCAGGCGTGGTGGGATCTCGATGCGAAaga < 1:3362794/69‑1 (MQ=255) gATGGAAGAAGTGGCAAAACGCGTTGAAGTTGACGGCATTCAGGCGTGGTGGGATCTCGATGCGAAaga < 1:2236105/69‑1 (MQ=255) gATGGAAGAAGTGGCAAAACGCGTTGAAGTTGACGGCATTCAGGCGTGGTGGGATCTCGATGCGAAaga < 1:226772/69‑1 (MQ=255) gATGGAAGAAGTGGCAAAACGCGTTGAAGTTGACGGCATTCAGGCGTGGTGGGATCTCGATGCGAAaga < 1:2419886/69‑1 (MQ=255) gATGGAAGAAGTGGCAAAACGCGTTGAAGTTGACGGCATTCAGGCGTGGTGGGATCTCGATGCGAAaga < 1:2308676/69‑1 (MQ=255) gATGGAAGAAGTGGCAAAACGCGTTGAAGTTGACGGCATTCAGGCGTGGTGGGATCTCGATGCGAAaga < 1:2923600/69‑1 (MQ=255) gATGGAAGAAGTGGCAAAACGCGTTGAAGTTGACGGCATTCAGGCGTGGTGGGATCTCGATGCGAAaga < 1:2358817/69‑1 (MQ=255) ggAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTTGTGGGATCTCGATGCGAAAGAGAt > 1:2968643/1‑69 (MQ=255) ggAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGAt > 1:470472/1‑69 (MQ=255) gaagaaGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGtggtggt < 1:3194857/49‑2 (MQ=255) gaagaaGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTggg < 1:2287758/49‑1 (MQ=255) gaagaaGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTggg < 1:685185/49‑1 (MQ=255) gaagaaGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTggg < 1:2371954/49‑1 (MQ=255) gaagaaGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTggg < 1:23164/49‑1 (MQ=255) gaagaaGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTggg < 1:471436/49‑1 (MQ=255) gaagaaGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTggg < 1:3435657/49‑1 (MQ=255) gaagaaGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTggg < 1:1859321/49‑1 (MQ=255) gaagaaGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTggg < 1:3021836/49‑1 (MQ=255) gaagaaGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTggg < 1:3175608/49‑1 (MQ=255) aagaagTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTggg < 1:2557749/48‑1 (MQ=255) ggCAAAACGCGTTGAAGTCGATGGCATCCAGGCgt < 1:208019/35‑1 (MQ=255) aCGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAg > 1:55307/1‑68 (MQ=255) aCGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAg > 1:1341681/1‑68 (MQ=255) gTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTa < 1:721391/68‑1 (MQ=255) | AAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTA > minE/21506‑21639 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |