| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,902,495 | G→C | 34.1% | V182V (GTC→GTG) | recB ← | exonuclease V (RecBCD complex), beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,902,495 | 0 | G | C | 34.1% | 33.8 / 29.2 | 41 | V182V (GTC→GTG) | recB | exonuclease V (RecBCD complex), beta subunit |
| Reads supporting (aligned to +/- strand): ref base G (8/19); new base C (7/7); total (15/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01 | |||||||||||
GCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGAC > minE/1902427‑1902535 | gcTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAgacg < 1:474399/69‑1 (MQ=255)gcTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAgacg < 1:2613601/69‑1 (MQ=255)gcTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAgacg < 1:1353290/69‑1 (MQ=255)gcTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAgacg < 1:531135/69‑1 (MQ=255)gcTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAgacg < 1:2981802/69‑1 (MQ=255) cTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAgacga < 1:2510363/69‑1 (MQ=255) cTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAgacga < 1:3103627/69‑1 (MQ=255) cTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAgacga < 1:1731513/69‑1 (MQ=255) tGCAGATACCGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCt < 1:2497673/69‑1 (MQ=255) tGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCt < 1:2871571/69‑1 (MQ=255) aGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACCACCTGGGCAAt < 1:1677939/68‑1 (MQ=255) aGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACCACCTGGGCAAt < 1:546076/68‑1 (MQ=255) aGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACCACCTGGGCAAt < 1:1922681/68‑1 (MQ=255) aGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACCACCTGGGCAAt < 1:3192673/68‑1 (MQ=255) aGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACCACCTGGGCAAt < 1:3343544/68‑1 (MQ=255) aGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACCACCTGGGCAAt < 1:415111/68‑1 (MQ=255) aGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACCACCTGGGCAAt < 1:659324/68‑1 (MQ=255) aTAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTc > 1:2615652/1‑69 (MQ=255) aTAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTc > 1:1031002/1‑69 (MQ=255) aTAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTc > 1:77147/1‑69 (MQ=255) aTAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTc > 1:2326513/1‑69 (MQ=255) tAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACCACCTGGGCAATTTCACGCGTCa > 1:982098/1‑69 (MQ=255) tAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACCACCTGGGCAATTTCACGCGTCa > 1:2840337/1‑69 (MQ=255) tAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACCACCTGGGCAATTTCACGCGTCa > 1:2207575/1‑69 (MQ=255) tAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACCACCTGGGCAATTTCACGCGTCa > 1:2205114/1‑69 (MQ=255) tAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACCACCTGGGCAATTTCACGCGTCa > 1:3344204/1‑69 (MQ=255) tAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACCACCTGGGCAATTTCACGCGTCa > 1:3191779/1‑69 (MQ=255) tAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACCACCTGGGCAATTTCACGCGTCa > 1:3273282/1‑69 (MQ=255) gcagcaACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGc < 1:3171391/69‑1 (MQ=255) gcagcaACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGc < 1:523077/69‑1 (MQ=255) gcagcaACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGc < 1:1422159/69‑1 (MQ=255) gcagcaACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGc < 1:3414423/69‑1 (MQ=255) gcagcaACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGc < 1:1513764/69‑1 (MQ=255) gcagcaACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGc < 1:1888128/69‑1 (MQ=255) gcagcaACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGc < 1:3098734/69‑1 (MQ=255) gcagcaACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGc < 1:2924623/69‑1 (MQ=255) gcagcaACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGc < 1:2789219/69‑1 (MQ=255) gcagcaACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGc < 1:2216720/69‑1 (MQ=255) cagcaACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGc < 1:748325/68‑1 (MQ=255) cagcaACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGc < 1:991288/68‑1 (MQ=255) agcaACGCCTGCGGCCCTTT‑‑AGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGc < 1:242933/65‑1 (MQ=255) caACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGc < 1:3432739/65‑1 (MQ=255) ccTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGAc > 1:811735/1‑69 (MQ=255) ccTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGAc > 1:90501/1‑69 (MQ=255) ccTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGAc > 1:1225100/1‑69 (MQ=255) ccTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGAc > 1:1221280/1‑69 (MQ=255) tCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGc < 1:650533/48‑1 (MQ=255) tCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGc < 1:1461794/48‑1 (MQ=255) aaaGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGc < 1:2130527/38‑1 (MQ=255) cgacgacCTGGGCTATTTCACGCGGCAGCGGGTAGc < 1:850194/35‑1 (MQ=255) gacgacCTGGGCTATTTCACGCGGCAGCGGGTAGc < 1:3153232/35‑1 (MQ=255) acgacCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGc < 1:86270/40‑1 (MQ=255) | GCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGAC > minE/1902427‑1902535 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |