| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 141,288 | A→T | 53.1% | T112T (ACT→ACA) | yadN ← | predicted fimbrial‑like adhesin protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 141,288 | 0 | A | T | 53.1% | 7.3 / 70.5 | 64 | T112T (ACT→ACA) | yadN | predicted fimbrial‑like adhesin protein |
| Reads supporting (aligned to +/- strand): ref base A (15/15); new base T (9/25); total (24/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.15e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
GTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGA > minE/141231‑141354 | gtgtAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGc < 1:948319/68‑1 (MQ=255)gtgtAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGc < 1:556465/68‑1 (MQ=255)gtgtAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGc < 1:1369399/68‑1 (MQ=255)gtgtAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGc < 1:3382588/68‑1 (MQ=255)gtgtAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCATTTGc < 1:2502055/68‑1 (MQ=255) tAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGt < 1:1372140/68‑1 (MQ=255) tAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGt < 1:88066/68‑1 (MQ=255) tAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGt < 1:465365/68‑1 (MQ=255) cGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTcc > 1:1428696/1‑54 (MQ=255) cGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTAc > 1:3108185/1‑66 (MQ=255) cGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTAc > 1:2496243/1‑66 (MQ=255) cGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAACTCCCTTTGCTGTTAc > 1:3241435/1‑66 (MQ=255) atTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGaa > 1:2050118/1‑69 (MQ=255) aaCGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGaa > 1:2135642/1‑66 (MQ=255) aaCGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGaa > 1:1008238/1‑66 (MQ=255) gttAATAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCAtttt < 1:2157117/69‑1 (MQ=255) gttAATAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCAtttt < 1:2473194/69‑1 (MQ=255) gttAATAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCAtttt < 1:2542390/69‑1 (MQ=255) gttAATAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCAtttt < 1:2713665/69‑1 (MQ=255) gttAATAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCAtttt < 1:380009/69‑1 (MQ=255) gttAATAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCAtttt < 1:2933179/69‑1 (MQ=255) gttAATAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCAtttt < 1:3101334/69‑1 (MQ=255) gttAATAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCAtttt < 1:3129938/69‑1 (MQ=255) gttAATAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCAtttt < 1:215615/69‑1 (MQ=255) gttAATAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCAtttt < 1:1089267/69‑1 (MQ=255) gttAATAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCAtttt < 1:753468/69‑1 (MQ=255) gttAATAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCAtttt < 1:1432358/69‑1 (MQ=255) gttAATAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCAtttt < 1:1348289/69‑1 (MQ=255) gttAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCAtttt < 1:664103/69‑1 (MQ=255) gttAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCAtttt < 1:732243/69‑1 (MQ=255) gttAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCAtttt < 1:92261/69‑1 (MQ=255) gttAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCAtttt < 1:151001/69‑1 (MQ=255) gttAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCAtttt < 1:753673/69‑1 (MQ=255) gttAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCAtttt < 1:13063/69‑1 (MQ=255) aaTAGACATGTCGTTGTTCGGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:2416876/69‑1 (MQ=255) aaTAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:37950/69‑1 (MQ=255) aaTAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:3116142/69‑1 (MQ=255) aaTAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:909803/69‑1 (MQ=255) aaTAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:3066429/69‑1 (MQ=255) aaTAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:2979815/69‑1 (MQ=255) aaTAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:754957/69‑1 (MQ=255) aaTAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:2914283/69‑1 (MQ=255) aaTAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:2021928/69‑1 (MQ=255) aTAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:3060745/68‑1 (MQ=255) aTAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:379633/68‑1 (MQ=255) tAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:3409462/67‑1 (MQ=255) cATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGCAGtagt > 1:747510/1‑66 (MQ=255) cATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGCAGtagt > 1:2886813/1‑66 (MQ=255) cATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGCAGtagt > 1:2931302/1‑66 (MQ=255) cATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGCAGtagt > 1:2866839/1‑66 (MQ=255) cATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGCAGtagt > 1:2139445/1‑66 (MQ=255) cATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGCAGtagt > 1:1545499/1‑66 (MQ=255) cATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGCAGtagt > 1:1460798/1‑66 (MQ=255) cATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGCAGtagt > 1:143881/1‑66 (MQ=255) cATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGCAGtagt > 1:1381223/1‑66 (MQ=255) gTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTAcc > 1:355765/1‑69 (MQ=255) gTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTAcc > 1:1805546/1‑69 (MQ=255) gTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTAcc > 1:1238580/1‑69 (MQ=255) gTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTAcc > 1:1353637/1‑69 (MQ=255) tCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTAcc > 1:3248453/1‑68 (MQ=255) tCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTAcc > 1:886182/1‑68 (MQ=255) tCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTAcc > 1:1686035/1‑68 (MQ=255) tCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTAcc > 1:677764/1‑68 (MQ=255) cAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCgg < 1:1787470/69‑1 (MQ=255) aaGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGa < 1:1055308/68‑1 (MQ=255) | GTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGA > minE/141231‑141354 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |