| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,914,053 | G→A | 28.0% | F473F (TTC→TTT) | ptsP ← | fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,914,053 | 0 | G | A | 28.0% | 112.5 / 42.9 | 103 | F473F (TTC→TTT) | ptsP | fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
| Reads supporting (aligned to +/- strand): ref base G (38/36); new base A (15/14); total (53/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 106 total aligned reads displayed.
GGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAG > minE/1913990‑1914121 | gggTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCTACCTGTTCTTCTTCCGACGGGAAAcc < 1:2293515/69‑1 (MQ=255)gggTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAAcc < 1:1096857/69‑1 (MQ=255)gggTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAAcc < 1:1626563/69‑1 (MQ=255)gggTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAAcc < 1:2131786/69‑1 (MQ=255)gggTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAAcc < 1:3420983/69‑1 (MQ=255)gggTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAAcc < 1:2565274/69‑1 (MQ=255)gggTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAAcc < 1:1358160/69‑1 (MQ=255)gggTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAAcc < 1:3219753/69‑1 (MQ=255) tttATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACt > 1:2901424/1‑69 (MQ=255) gAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTgcagca > 1:1269604/1‑68 (MQ=255) aCATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATg < 1:3147597/68‑1 (MQ=255) aCATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATg < 1:2985318/68‑1 (MQ=255) aCATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATg < 1:1875403/68‑1 (MQ=255) aCATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATg < 1:1081789/68‑1 (MQ=255) aCATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGAAAACCACTTTGCAGCATGa > 1:3175018/1‑69 (MQ=255) aCATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGAAAACCACTTTGCAGCATGa > 1:695784/1‑69 (MQ=255) aCATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGAAAACCACTTTGCAGCATGa > 1:2074476/1‑69 (MQ=255) aCATTTGCAGCATCCCCTGATAATGCGCCACCTGTTCTTCTTCCGACGGAAAACCACttt > 1:1503931/1‑60 (MQ=255) tttGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGAAAACCACTTTGCAGCATGAATg < 1:1830273/69‑1 (MQ=255) tttGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGAAAACCACTTTGCAGCATGAATg < 1:1312534/69‑1 (MQ=255) tttGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGAAAACCACTTTGCAGCATGAATg < 1:1647646/69‑1 (MQ=255) tttGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGAAAACCACTTTGCAGCATGAATg < 1:448586/69‑1 (MQ=255) tttGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGAAAACCACTTTGCAGCATGAATg < 1:1568683/69‑1 (MQ=255) tttGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGAAAACCACTTTGCAGCATGAATg < 1:436722/69‑1 (MQ=255) tttGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGAAAACCACTTTGCAGCATGAATg < 1:2507255/69‑1 (MQ=255) tttGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGAAAACCACTTTGCAGCATGAATg < 1:3246785/69‑1 (MQ=255) tttGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGAAAACCACTTTGCAGCATGAATg < 1:2814325/69‑1 (MQ=255) tttGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGAAAACCACTTTGCAGCATGAATg < 1:2837136/69‑1 (MQ=255) tttGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGAAAACCACTTTGCAGCATGAATg < 1:2887361/69‑1 (MQ=255) tttGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGAAAACCACTTTGCAGCATGAATg < 1:2978665/69‑1 (MQ=255) caTCCCCTGATACTGCGCCACCTGTTCTTCTTCTGACGGGAAACCACTTTGCAGCATGAATGGGATTTc < 1:22159/69‑1 (MQ=255) caTCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTc < 1:2497579/69‑1 (MQ=255) caTCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTc < 1:809677/69‑1 (MQ=255) caTCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTc < 1:2365930/69‑1 (MQ=255) caTCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTc < 1:1387936/69‑1 (MQ=255) caTCCCATGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTc < 1:2250301/69‑1 (MQ=255) cccTGATACTGCGCCACCTGTTCTTCTTCCGACGGAAAACCACTTTGCAGCATGAATg < 1:3411266/58‑1 (MQ=255) gATACTGCGCCACCTGTTCTTCTTCCGACTGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGa > 1:2927398/1‑68 (MQ=255) aCTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATa < 1:1553690/67‑1 (MQ=255) aCTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATa < 1:3327962/67‑1 (MQ=255) cTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGAt > 1:2560111/1‑54 (MQ=255) cTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGAt > 1:1449154/1‑54 (MQ=255) cTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGAt > 1:1388248/1‑54 (MQ=255) cTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGAt > 1:1141103/1‑54 (MQ=255) cTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGAt > 1:466227/1‑54 (MQ=255) gcgcCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAg > 1:1346475/1‑67 (MQ=255) gcgcCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGa > 1:1197068/1‑68 (MQ=255) gcgcCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGa > 1:42081/1‑68 (MQ=255) gcgcCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGa > 1:1838453/1‑68 (MQ=255) gcgcCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGa > 1:498402/1‑68 (MQ=255) gcgcCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGa > 1:1437634/1‑68 (MQ=255) gcgcCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGa > 1:687361/1‑68 (MQ=255) gcgcCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGa > 1:2181135/1‑68 (MQ=255) gcgcCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGa > 1:1634931/1‑68 (MQ=255) gcgcCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGa > 1:2334011/1‑68 (MQ=255) ccACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGTTTTCAGTGCGATAAAGACCGa < 1:2801871/69‑1 (MQ=255) ccACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGa < 1:3426965/69‑1 (MQ=255) ccACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGa < 1:125734/69‑1 (MQ=255) ccACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGa < 1:1951965/69‑1 (MQ=255) cACCTGTTCTTCTTCCGACGGAAAACCACTTTGCAGCATGAATg < 1:621850/44‑1 (MQ=255) ccTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGc > 1:3190814/1‑69 (MQ=255) ccTGTCCTTCTTCCGACGGGAATCCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGc > 1:3123671/1‑69 (MQ=255) tGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATg > 1:2706113/1‑36 (MQ=255) tGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATg > 1:1288667/1‑36 (MQ=255) tGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATaaa < 1:840315/58‑1 (MQ=255) tGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATaaa < 1:823494/58‑1 (MQ=255) tGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATaaa < 1:2156605/58‑1 (MQ=255) tGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATaaa < 1:3381082/58‑1 (MQ=255) tGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATaaa < 1:2992477/58‑1 (MQ=255) tGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATaaa < 1:217886/58‑1 (MQ=255) tGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGcc > 1:405756/1‑68 (MQ=255) tGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGcc > 1:696891/1‑68 (MQ=255) tGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGcc > 1:571033/1‑68 (MQ=255) tGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGcc > 1:1326334/1‑68 (MQ=255) tGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGcc > 1:2651392/1‑68 (MQ=255) tGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGcc > 1:534881/1‑68 (MQ=255) tGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGcc > 1:1476579/1‑68 (MQ=255) tGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGc > 1:35012/1‑67 (MQ=255) tGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGc > 1:2710306/1‑67 (MQ=255) tGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATAGGATTTCAGTGCGATAAAGACCGAt > 1:1351841/1‑65 (MQ=255) ttcttcttcCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATc > 1:1381028/1‑69 (MQ=255) ttcttcttcCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATc > 1:1585130/1‑69 (MQ=255) ttcttcttcCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATc > 1:2888010/1‑69 (MQ=255) ttcttcttcCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATc > 1:586845/1‑69 (MQ=255) cttcttcCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCaa < 1:887716/69‑1 (MQ=255) cttcttcCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCaa < 1:2528679/69‑1 (MQ=255) cttcttcCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCaa < 1:1847891/69‑1 (MQ=255) cttcttcCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCaa < 1:141044/69‑1 (MQ=255) ccGACGGAAAACCACTTTGCAGCATGAATGGGATTTCAGt > 1:810086/1‑40 (MQ=255) ccGACGGAAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGTCCTATGCCATCAATACGGc > 1:2322115/1‑69 (MQ=255) ccGACGGAAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGTCCTATGCCATCAATACGGc > 1:2207469/1‑69 (MQ=255) ccGACGGAAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGTCCTATGCCATCAATACGGc > 1:659346/1‑69 (MQ=255) ccGACGGAAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGTCCTATGCCATCAATACGGc > 1:1839343/1‑69 (MQ=255) ccGACGGAAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGTCCTATGCCATCAATACGGc > 1:1806032/1‑69 (MQ=255) ccGACGGAAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGTCCTATGCCATCAATACGGc > 1:1760500/1‑69 (MQ=255) ccGACGGAAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGTCCTATGCCATCAATACGGc > 1:1189679/1‑69 (MQ=255) ccGACGGAAAACAACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGTCCTATGCCATCAATACGGc > 1:1151098/1‑69 (MQ=255) gAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAg < 1:2587446/69‑1 (MQ=255) gAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAg < 1:566810/69‑1 (MQ=255) gAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAg < 1:871905/69‑1 (MQ=255) | GGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAG > minE/1913990‑1914121 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |