| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,914,338 | C→T | 51.8% | V378V (GTG→GTA) | ptsP ← | fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,914,338 | 0 | C | T | 51.8% | ‑1.9 / 73.1 | 81 | V378V (GTG→GTA) | ptsP | fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
| Reads supporting (aligned to +/- strand): ref base C (12/27); new base T (21/21); total (33/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGAT > minE/1914279‑1914397 | tCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAgg > 1:1425310/1‑68 (MQ=255)tCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAgg > 1:179553/1‑68 (MQ=255)tCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAgg > 1:2342350/1‑68 (MQ=255)tCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAgg > 1:487780/1‑68 (MQ=255)tCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAgg > 1:512899/1‑68 (MQ=255) gATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTg < 1:531935/67‑1 (MQ=255) gATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTg < 1:446821/67‑1 (MQ=255) gATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTg < 1:418726/67‑1 (MQ=255) gATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTg < 1:1533732/67‑1 (MQ=255) gATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTg < 1:2009957/67‑1 (MQ=255) gATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTg < 1:819047/67‑1 (MQ=255) ttCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCAt < 1:563754/69‑1 (MQ=255) ttCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCAt < 1:2476361/69‑1 (MQ=255) ttCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCAt < 1:3271468/69‑1 (MQ=255) ttCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCAt < 1:2554926/69‑1 (MQ=255) ttCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCAt < 1:2492165/69‑1 (MQ=255) ttCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCAt < 1:760248/69‑1 (MQ=255) ttCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCAt < 1:2249118/69‑1 (MQ=255) ttCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCAt < 1:1865159/69‑1 (MQ=255) ttCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCAt < 1:1160491/69‑1 (MQ=255) ttCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCAt < 1:1175943/69‑1 (MQ=255) tCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCAt < 1:20284/68‑1 (MQ=255) cgcgATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACgg < 1:604254/69‑1 (MQ=255) gCCATCTACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGAt > 1:440339/1‑69 (MQ=255) gCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGAt > 1:2855097/1‑69 (MQ=255) gCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGAt > 1:1482446/1‑69 (MQ=255) gCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGAt > 1:3391775/1‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATcccc > 1:3315943/3‑68 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATcccc > 1:3259727/3‑68 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATcccc > 1:2359381/3‑68 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATcccc > 1:1016943/3‑68 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATcccc > 1:1799261/3‑68 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:1478724/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:1405008/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:3328164/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:343435/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:2927404/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:517612/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:2512023/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:3370822/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:2412979/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:1081603/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:1892668/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:1916396/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:1961518/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:2221339/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:2438543/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:2315435/3‑69 (MQ=255) gTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTAcc < 1:2663795/69‑1 (MQ=255) gTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTAcc < 1:2245738/69‑1 (MQ=255) gTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTAcc < 1:2022238/69‑1 (MQ=255) tCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTAcc > 1:1226538/1‑68 (MQ=255) tCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTAcc > 1:1526735/1‑68 (MQ=255) tCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTAcc > 1:216206/1‑68 (MQ=255) cTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATg < 1:2631243/69‑1 (MQ=255) cTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATg < 1:1807866/69‑1 (MQ=255) cTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATg < 1:1721575/69‑1 (MQ=255) cTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATg < 1:487107/69‑1 (MQ=255) cTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATg < 1:1217682/69‑1 (MQ=255) tACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATg < 1:3365562/68‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGGGCACGTACCATGAt < 1:2509936/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:1257841/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:675578/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:383692/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:714774/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:2760007/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:365240/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:3372417/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:3062489/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:3016910/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:300669/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:2809585/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:1775170/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:2698171/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:254747/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:2461818/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:2461710/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:240377/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:1565692/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:1714123/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:1736567/69‑1 (MQ=255) gcagtaCCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:1022768/64‑1 (MQ=255) | TCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGAT > minE/1914279‑1914397 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |