| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,914,374 | G→T | 73.1% | I366I (ATC→ATA) | ptsP ← | fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,914,374 | 0 | G | T | 73.1% | 114.2 / 53.8 | 93 | I366I (ATC→ATA) | ptsP | fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
| Reads supporting (aligned to +/- strand): ref base G (14/11); new base T (39/29); total (53/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
GCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACA > minE/1914308‑1914437 | gCCATCTACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGAt > 1:440339/1‑69 (MQ=255)gCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGAt > 1:2855097/1‑69 (MQ=255)gCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGAt > 1:3391775/1‑69 (MQ=255)gCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGAt > 1:1482446/1‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATcccc > 1:1016943/3‑68 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATcccc > 1:2359381/3‑68 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATcccc > 1:3259727/3‑68 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATcccc > 1:3315943/3‑68 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATcccc > 1:1799261/3‑68 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:1405008/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:2927404/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:2315435/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:2412979/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:2221339/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:517612/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:3328164/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:3370822/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:2438543/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:1961518/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:1916396/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:1081603/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:1892668/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:343435/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:1478724/3‑69 (MQ=255) cgaCGATCAGCGTCCGACGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCa > 1:2512023/3‑69 (MQ=255) gTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTAcc < 1:2022238/69‑1 (MQ=255) gTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTAcc < 1:2245738/69‑1 (MQ=255) gTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTAcc < 1:2663795/69‑1 (MQ=255) tCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTAcc > 1:1526735/1‑68 (MQ=255) tCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTAcc > 1:216206/1‑68 (MQ=255) tCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTAcc > 1:1226538/1‑68 (MQ=255) cTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATg < 1:2631243/69‑1 (MQ=255) cTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATg < 1:1807866/69‑1 (MQ=255) cTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATg < 1:1217682/69‑1 (MQ=255) cTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATg < 1:487107/69‑1 (MQ=255) cTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATg < 1:1721575/69‑1 (MQ=255) tACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATg < 1:3365562/68‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGGGCACGTACCATGAt < 1:2509936/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:2698171/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:240377/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:2809585/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:2760007/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:254747/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:2461818/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:2461710/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:300669/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:3016910/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:3062489/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:3372417/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:365240/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:383692/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:1775170/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:1736567/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:1714123/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:1565692/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:1257841/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:675578/69‑1 (MQ=255) aCGATGCAGTACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:714774/69‑1 (MQ=255) gcagtaCCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGAt < 1:1022768/64‑1 (MQ=255) taCCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTa > 1:1390038/2‑53 (MQ=255) taCCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCgcag > 1:2324544/2‑65 (MQ=255) taCCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCgcag > 1:1860074/2‑65 (MQ=255) taCCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCgcag > 1:1054877/2‑65 (MQ=255) taCCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCgcag > 1:1856136/2‑65 (MQ=255) taCCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCgcag > 1:1832666/2‑65 (MQ=255) taCCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCgcag > 1:406454/2‑65 (MQ=255) taCCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCgcag > 1:2038591/2‑65 (MQ=255) taCCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCgcag > 1:419021/2‑65 (MQ=255) taCCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCgcag > 1:22533/2‑65 (MQ=255) taCCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCgcag > 1:2341553/2‑65 (MQ=255) taCCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCgcag > 1:921839/2‑65 (MQ=255) taCCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCgcag > 1:1411752/2‑65 (MQ=255) taCCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCgcag > 1:2355007/2‑65 (MQ=255) taCCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCgcag > 1:2379712/2‑65 (MQ=255) taCCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCgcag > 1:663231/2‑65 (MQ=255) taCCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCgcag > 1:2698214/2‑65 (MQ=255) taCCGAAGGCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCgca > 1:2279207/2‑64 (MQ=255) gCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTgg < 1:2009079/69‑1 (MQ=255) gCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTgg < 1:3416747/69‑1 (MQ=255) gCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTgg < 1:1905059/69‑1 (MQ=255) gCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTgg < 1:3364403/69‑1 (MQ=255) gCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTgg < 1:2448876/69‑1 (MQ=255) gCTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTgg < 1:2774255/69‑1 (MQ=255) cTGAATATCCGCGCCCATCACGGTAGGTATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTgg < 1:203022/68‑1 (MQ=255) tatCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTgcgc < 1:2938617/69‑1 (MQ=255) aTCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACg < 1:557699/69‑1 (MQ=255) aCGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGac > 1:2141494/1‑68 (MQ=255) aCGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGac > 1:1069477/1‑68 (MQ=255) aCGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGac > 1:1065481/1‑68 (MQ=255) gTATGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGacaaca > 1:3428839/1‑69 (MQ=255) gTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGacaaca > 1:2652290/1‑69 (MQ=255) gTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGacaaca > 1:2951275/1‑69 (MQ=255) aGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTgg > 1:2008862/1‑44 (MQ=255) | GCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACA > minE/1914308‑1914437 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |