| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,920,411 | G→A | 27.3% | V163V (GTC→GTT) ‡ | ygeD ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,920,411 | 0 | G | A | 27.3% | 92.9 / 34.4 | 84 | V163V (GTC→GTT) ‡ | ygeD | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (37/24); new base A (4/19); total (41/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.99e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
TGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTC > minE/1920344‑1920479 | tGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGa < 1:2423409/69‑1 (MQ=255)tGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGa < 1:2843630/69‑1 (MQ=255)tGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGa < 1:3410844/69‑1 (MQ=255) tGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGcc > 1:519682/1‑69 (MQ=255) tAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGCCGAGGACATGCCa > 1:1798736/1‑68 (MQ=255) tAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCa > 1:2458110/1‑68 (MQ=255) tAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCa > 1:2863233/1‑68 (MQ=255) tAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCa > 1:1321901/1‑68 (MQ=255) tAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCa > 1:1592896/1‑68 (MQ=255) tAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCa > 1:885825/1‑68 (MQ=255) tatTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTca < 1:965174/68‑1 (MQ=255) tatTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTca < 1:422623/68‑1 (MQ=255) tatTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTca < 1:3271583/68‑1 (MQ=255) tatTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTca < 1:846421/68‑1 (MQ=255) tatTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTca < 1:1623960/68‑1 (MQ=255) ttGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTcagcc > 1:1752968/1‑69 (MQ=255) ttGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTcagcc > 1:776595/1‑69 (MQ=255) ttGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTcagcc > 1:3362523/1‑69 (MQ=255) ttGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTcagcc > 1:2824164/1‑69 (MQ=255) ggCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTcagccag > 1:669932/1‑69 (MQ=255) ggCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTcagccag > 1:30587/1‑69 (MQ=255) ggCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTcagccag > 1:3195191/1‑69 (MQ=255) ggCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTcagccag > 1:2281676/1‑69 (MQ=255) ggCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTcagccag > 1:540462/1‑69 (MQ=255) ggCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTcagccag > 1:2128714/1‑69 (MQ=255) ggCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTcagccag > 1:419981/1‑69 (MQ=255) ggCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTcagccag > 1:954076/1‑69 (MQ=255) aaCGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGcac < 1:482037/69‑1 (MQ=255) cGCACCACCGTAGGCCAGTTCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCAcaccac > 1:288377/1‑68 (MQ=255) cGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGcac < 1:1820520/63‑1 (MQ=255) cGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACaccacc > 1:2805444/1‑69 (MQ=255) cGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACaccacc > 1:175435/1‑69 (MQ=255) accGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATg < 1:1977203/42‑1 (MQ=255) accGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATg < 1:599652/42‑1 (MQ=255) accGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATg < 1:248103/42‑1 (MQ=255) accGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATg < 1:3389164/42‑1 (MQ=255) accGTAGGCCAGTGCGCATGCGGCCAGGGCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTa > 1:2559912/1‑68 (MQ=255) accGTAGGCCAGTGCGCATGCGGCCAGGGCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTa > 1:3017972/1‑68 (MQ=255) accGTAGGCCAGTGCGCATGCGGCCAGGGCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTa > 1:1055469/1‑68 (MQ=255) accGTAGGCCAGTGCGCATGCGGCCAGGGCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTa > 1:1182093/1‑68 (MQ=255) cGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGa < 1:969802/36‑1 (MQ=255) tGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCaa > 1:2356292/1‑69 (MQ=255) tGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCaa > 1:1180425/1‑69 (MQ=255) tGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCaa > 1:1286423/1‑69 (MQ=255) tGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCaa > 1:75424/1‑69 (MQ=255) tGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCaa > 1:1850487/1‑69 (MQ=255) tGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCaa > 1:1900454/1‑69 (MQ=255) tGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCaa > 1:1937302/1‑69 (MQ=255) tGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCaa > 1:2330254/1‑69 (MQ=255) tGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCaa > 1:499807/1‑69 (MQ=255) cgcATGCGGCCAGGGCGCCGAGGAAATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCaaaa > 1:1479506/1‑69 (MQ=255) cgcATGCGGCCAGGGCGACGAGGAAATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCaaaa > 1:1483272/1‑69 (MQ=255) cGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGa < 1:251683/52‑1 (MQ=255) cGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATcgcc > 1:1815907/1‑68 (MQ=255) cGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATcgcc > 1:724788/1‑68 (MQ=255) cGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATcgcc > 1:1457340/1‑68 (MQ=255) cGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATcgcc > 1:2510034/1‑68 (MQ=255) gCCAGGGCTACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCt < 1:1088963/69‑1 (MQ=255) gCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATcgccgc < 1:1612537/68‑1 (MQ=255) gCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATcgccgc < 1:2169492/68‑1 (MQ=255) gCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCt < 1:3007670/69‑1 (MQ=255) gCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCt < 1:1161667/69‑1 (MQ=255) gCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCt < 1:500842/69‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:618782/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:3038177/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:1200869/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:1608864/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:805889/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:28394/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:77658/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:775523/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:1612658/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:728535/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:1966193/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:3294457/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:3219201/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:2283442/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:506493/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:2821113/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:2467480/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:2752026/63‑1 (MQ=255) gggCAACCAGAATATGCCAGGCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:2716555/63‑1 (MQ=255) acgaGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGaa < 1:2147071/69‑1 (MQ=255) acgaGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGaa < 1:2396357/69‑1 (MQ=255) gACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTc < 1:1617154/69‑1 (MQ=255) gACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTc < 1:2544185/69‑1 (MQ=255) gACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTc < 1:2641181/69‑1 (MQ=255) gACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTc < 1:2690746/69‑1 (MQ=255) gACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTc < 1:3398338/69‑1 (MQ=255) gACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTc < 1:1602514/69‑1 (MQ=255) gACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTc < 1:1595725/69‑1 (MQ=255) | TGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTC > minE/1920344‑1920479 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |