| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,920,438 | G→A | 31.4% | A154A (GCC→GCT) | ygeD ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,920,438 | 0 | G | A | 31.4% | 66.5 / 33.8 | 73 | A154A (GCC→GCT) | ygeD | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (24/26); new base A (4/19); total (28/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.88e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01 | |||||||||||
ACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTCCATTAAACCGTTAGCTTTCACTAACTT > minE/1920375‑1920506 | accGTAGGCCAGTGCGCATGCGGCCAGGGCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTa > 1:3017972/1‑68 (MQ=255)accGTAGGCCAGTGCGCATGCGGCCAGGGCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTa > 1:1055469/1‑68 (MQ=255)accGTAGGCCAGTGCGCATGCGGCCAGGGCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTa > 1:2559912/1‑68 (MQ=255)accGTAGGCCAGTGCGCATGCGGCCAGGGCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTa > 1:1182093/1‑68 (MQ=255) tGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCaa > 1:1937302/1‑69 (MQ=255) tGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCaa > 1:2356292/1‑69 (MQ=255) tGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCaa > 1:2330254/1‑69 (MQ=255) tGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCaa > 1:499807/1‑69 (MQ=255) tGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCaa > 1:1900454/1‑69 (MQ=255) tGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCaa > 1:1850487/1‑69 (MQ=255) tGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCaa > 1:75424/1‑69 (MQ=255) tGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCaa > 1:1180425/1‑69 (MQ=255) tGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCaa > 1:1286423/1‑69 (MQ=255) cgcATGCGGCCAGGGCGCCGAGGAAATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCaaaa > 1:1479506/1‑69 (MQ=255) cgcATGCGGCCAGGGCGACGAGGAAATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCaaaa > 1:1483272/1‑69 (MQ=255) cGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGa < 1:251683/52‑1 (MQ=255) cGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATcgcc > 1:1457340/1‑68 (MQ=255) cGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATcgcc > 1:2510034/1‑68 (MQ=255) cGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATcgcc > 1:724788/1‑68 (MQ=255) cGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATcgcc > 1:1815907/1‑68 (MQ=255) gCCAGGGCTACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCt < 1:1088963/69‑1 (MQ=255) gCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATcgccgc < 1:1612537/68‑1 (MQ=255) gCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATcgccgc < 1:2169492/68‑1 (MQ=255) gCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCt < 1:3007670/69‑1 (MQ=255) gCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCt < 1:500842/69‑1 (MQ=255) gCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCt < 1:1161667/69‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:1612658/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:775523/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:3038177/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:3219201/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:28394/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:2821113/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:2752026/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:3294457/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:805889/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:2467480/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:728535/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:2283442/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:1608864/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:618782/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:77658/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:1200869/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:1966193/63‑1 (MQ=255) gggCAACCAGAATATGCCAGTCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:506493/63‑1 (MQ=255) gggCAACCAGAATATGCCAGGCAGCCAGAACACCACCAGCTACGGAACCGAGCAAAATcgccg < 1:2716555/63‑1 (MQ=255) acgaGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGaa < 1:2396357/69‑1 (MQ=255) acgaGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGaa < 1:2147071/69‑1 (MQ=255) gACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTc < 1:1617154/69‑1 (MQ=255) gACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTc < 1:2690746/69‑1 (MQ=255) gACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTc < 1:2641181/69‑1 (MQ=255) gACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTc < 1:2544185/69‑1 (MQ=255) gACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTc < 1:1595725/69‑1 (MQ=255) gACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTc < 1:1602514/69‑1 (MQ=255) gACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTc < 1:3398338/69‑1 (MQ=255) aCATGCCAGTCAGCCAGCACACCACCGGCTACGAAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTc < 1:97343/68‑1 (MQ=255) ccAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCt > 1:157762/1‑49 (MQ=255) ccAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTa > 1:1142935/1‑50 (MQ=255) ccAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTAt > 1:2925361/1‑51 (MQ=255) ccAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTAt > 1:840263/1‑51 (MQ=255) ccAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTAt > 1:1238103/1‑51 (MQ=255) ccAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTAt > 1:1605094/1‑51 (MQ=255) ccAGTCAGCCAGCACACCACCGGCTACGGAACAGAGCAAAATCGCCGCTAt > 1:2188868/1‑51 (MQ=255) cagccagcACACCACCGGCTACGGAACCGAGCAAAATcgccgc < 1:2188270/43‑1 (MQ=255) cagccagcACACCACCGGCTACGGAACCGAGCAAAATcgccgc < 1:520011/43‑1 (MQ=255) cagccagcACACCACCGGCTACGGAACCGAGCAAAATcgccgc < 1:1889120/43‑1 (MQ=255) cagccagcACACCACCGGCTACGGAACCGAGCAAAATcgccgc < 1:68454/43‑1 (MQ=255) cagccagcACACCACCGGCTACGGAACCGAGCAAAATcgccgc < 1:1866859/43‑1 (MQ=255) cagccagcACACCACCGGCTACGGAACCGAGCAAAATcgccgc < 1:2368766/43‑1 (MQ=255) cagccagcACACCACCGGCTACGGAACCGAGCAAAATcgccgc < 1:1568162/43‑1 (MQ=255) cagccagcACACCACCGGCTACGGAACCGAGCAAAATcgccgc < 1:1149006/43‑1 (MQ=255) cagccagcACACCACCGGCTACGGAACCGAGCAAAATcgccgc < 1:2850871/43‑1 (MQ=255) cagccagcACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTCCATTAAAcc > 1:2244264/1‑67 (MQ=255) agccagcACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTc > 1:3033605/1‑57 (MQ=255) ggCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTCCATTAAACCGTTAGCTTTCACTAACtt < 1:2653235/69‑1 (MQ=255) | ACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTCCATTAAACCGTTAGCTTTCACTAACTT > minE/1920375‑1920506 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |