| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,924,613 | T→C | 24.9% | R326R (CGT→CGC) | galR → | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,924,613 | 0 | T | C | 24.9% | 99.7 / 28.9 | 84 | R326R (CGT→CGC) | galR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (39/24); new base C (7/14); total (46/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.13e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAA > minE/1924545‑1924677 | ttGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTAcgtcgt < 1:1132843/69‑1 (MQ=255) gcTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTc > 1:2146506/1‑69 (MQ=255) cTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCa < 1:1609288/69‑1 (MQ=255) tcCCGGAAATCACTGATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTgg > 1:3199239/1‑69 (MQ=255) tcCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTgg > 1:397319/1‑69 (MQ=255) tcCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTgg > 1:3313374/1‑69 (MQ=255) tcCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTAAGTGTCAACTCCGTCGCTgg > 1:1684450/1‑69 (MQ=255) aaTCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCaa > 1:926894/1‑68 (MQ=255) aaTCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCaa > 1:2964007/1‑68 (MQ=255) aaTCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCaa > 1:390990/1‑68 (MQ=255) aaTCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCaa > 1:1783575/1‑68 (MQ=255) aaTCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCaa > 1:1729639/1‑68 (MQ=255) aCTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGtcat < 1:3169448/69‑1 (MQ=255) aCTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGtcat < 1:2696360/69‑1 (MQ=255) aCTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGtcat < 1:2235901/69‑1 (MQ=255) aCTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGtcat < 1:186177/69‑1 (MQ=255) aCTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGtcat < 1:3432738/69‑1 (MQ=255) aCTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGtcat < 1:1410066/69‑1 (MQ=255) aTGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTcatcat > 1:1938260/1‑68 (MQ=255) gTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCa < 1:3191795/69‑1 (MQ=255) gTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCa < 1:2024871/69‑1 (MQ=255) gTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCa < 1:3165141/69‑1 (MQ=255) gTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCa < 1:1917682/69‑1 (MQ=255) gTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCa < 1:3070475/69‑1 (MQ=255) tCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATgta > 1:2010254/1‑66 (MQ=255) tCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCa > 1:2592182/1‑68 (MQ=255) tCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCa > 1:2802990/1‑68 (MQ=255) tCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCa > 1:663196/1‑68 (MQ=255) tCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCa > 1:3052139/1‑68 (MQ=255) tttAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGtcat > 1:1890006/1‑60 (MQ=255) tttAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGtcat > 1:3088414/1‑60 (MQ=255) tttAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGtcat > 1:1335767/1‑60 (MQ=255) tttAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGtcat > 1:2121388/1‑60 (MQ=255) tttAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGtcat > 1:548814/1‑60 (MQ=255) tttAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGtcat > 1:2184103/1‑60 (MQ=255) tttAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGtcat > 1:2209970/1‑60 (MQ=255) tttAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGtcat > 1:2211213/1‑60 (MQ=255) tttAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGtcat > 1:1547485/1‑60 (MQ=255) tttAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGtcat > 1:1573471/1‑60 (MQ=255) tttAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGtcat > 1:727861/1‑60 (MQ=255) tttAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGtcat > 1:1646667/1‑60 (MQ=255) tttAGTCCGACGCTGGTACGTCGCCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGc > 1:1017490/1‑65 (MQ=255) tttAGTCCGACGCTGGTACGTCGCCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAAcc > 1:1569993/1‑69 (MQ=255) tttAGTCCGACGCTGGTACGTCGCCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAAcc > 1:3436039/1‑69 (MQ=255) tttAGTCCGACGCTGGTACGTCGCCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAAcc > 1:3239863/1‑69 (MQ=255) tttAGTCCGACGCTGGTACGTCGCCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAAcc > 1:1614964/1‑69 (MQ=255) tttAGTCCGACGCTGGTACGTCGCCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAAcc > 1:36776/1‑69 (MQ=255) tttAGTCCGACGCTGGTACGTCGCCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAAcc > 1:3040003/1‑69 (MQ=255) tttAGTCCGACACTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGtcat > 1:613011/1‑60 (MQ=255) tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATGATGCAACCAGCg < 1:916140/68‑1 (MQ=255) tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCg < 1:1010338/68‑1 (MQ=255) tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCg < 1:1587857/68‑1 (MQ=255) tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCg < 1:402765/68‑1 (MQ=255) tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCg < 1:1874783/68‑1 (MQ=255) tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCg < 1:1289510/68‑1 (MQ=255) tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCg < 1:2020780/68‑1 (MQ=255) tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCg < 1:2853434/68‑1 (MQ=255) tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCg < 1:2822149/68‑1 (MQ=255) tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCg < 1:918548/68‑1 (MQ=255) cGCTGGTACGTCGCCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAAc < 1:1304438/69‑1 (MQ=255) cGCTGGTACGTCGCCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAAc < 1:422925/69‑1 (MQ=255) cGCTGGTACGTCGCCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAAc < 1:677992/69‑1 (MQ=255) cGCTGGTACGTCGCCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAAc < 1:1406894/69‑1 (MQ=255) cGCTGGTACGTCGCCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAAc < 1:1450337/69‑1 (MQ=255) cGCTGGTACGTCGCCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAAc < 1:1274596/69‑1 (MQ=255) cGCTGGTACGTCGCCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAAc < 1:950308/69‑1 (MQ=255) cGCTGGTACGTCGCCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAAc < 1:3313036/69‑1 (MQ=255) cGCTGGTACGTCGCCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAAc < 1:3307600/69‑1 (MQ=255) cGCTGGTACGTCGCCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAAc < 1:1826458/69‑1 (MQ=255) cGCTGGTACGTCGCCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAAc < 1:1832559/69‑1 (MQ=255) cGCTGGTACGTCGCCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAAc < 1:3163973/69‑1 (MQ=255) cGCTGGTACGTCGCCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAAc < 1:2845441/69‑1 (MQ=255) cGCTGGTACGTCGCCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAAc < 1:2013332/69‑1 (MQ=255) gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGTAGGCAAGTCATCATGCAACCAGCGACTAAc > 1:3096308/1‑68 (MQ=255) gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCGACCAGCGACTAAc > 1:3002967/1‑68 (MQ=255) gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAAc > 1:903960/1‑68 (MQ=255) gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAAc > 1:791201/1‑68 (MQ=255) gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAAc > 1:747/1‑68 (MQ=255) gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAAc > 1:907957/1‑68 (MQ=255) gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAAc > 1:703436/1‑68 (MQ=255) gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAAc > 1:3380959/1‑68 (MQ=255) gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAAc > 1:1919546/1‑68 (MQ=255) gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAAc > 1:2293466/1‑68 (MQ=255) aCGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGt < 1:3038549/68‑1 (MQ=255) aCGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGt < 1:464357/68‑1 (MQ=255) tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCGGTTaa < 1:231699/68‑1 (MQ=255) tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTaa < 1:2851633/68‑1 (MQ=255) tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTaa < 1:1640262/68‑1 (MQ=255) tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTaa < 1:229925/68‑1 (MQ=255) | TTGGCGCTGGCGGATAATCGCCCTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAA > minE/1924545‑1924677 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |