| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,924,968 | A→C | 35.2% | D323E (GAT→GAG) | lysA ← | diaminopimelate decarboxylase, PLP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,924,968 | 0 | A | C | 35.2% | 62.8 / 73.6 | 88 | D323E (GAT→GAG) | lysA | diaminopimelate decarboxylase, PLP‑binding |
| Reads supporting (aligned to +/- strand): ref base A (6/51); new base C (23/8); total (29/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.97e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAA > minE/1924909‑1925033 | tCACATAACGGCCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGCCCCTCAGCTGcc > 1:2235574/1‑69 (MQ=255)tCACATAACGGCCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCCTCAGCTGcc > 1:3261430/1‑69 (MQ=255)tCACATAACGGCCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCCTCAGCTGcc > 1:2721647/1‑69 (MQ=255)tCACATAACGGCCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCCTCAGCTGcc > 1:790344/1‑69 (MQ=255)tCACATAACGGCCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCCTCAGCTGcc > 1:1521319/1‑69 (MQ=255) caTAACGGTCC‑‑AGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAggg < 1:2983830/68‑1 (MQ=255) aTAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAggg < 1:453020/69‑1 (MQ=255) aTAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAggg < 1:2916216/69‑1 (MQ=255) aTAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAggg < 1:1665308/69‑1 (MQ=255) aTAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAggg < 1:57076/69‑1 (MQ=255) aTAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAggg < 1:629528/69‑1 (MQ=255) aTAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAggg < 1:1477934/69‑1 (MQ=255) aTAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAggg < 1:1284531/69‑1 (MQ=255) gTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGa < 1:706200/69‑1 (MQ=255) gTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGa < 1:2668428/69‑1 (MQ=255) gTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGa < 1:1760217/69‑1 (MQ=255) gTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGa < 1:2917759/69‑1 (MQ=255) tCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGa < 1:1756557/68‑1 (MQ=255) tCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGa < 1:2583988/68‑1 (MQ=255) tCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGa < 1:2489254/68‑1 (MQ=255) cGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGatat < 1:1022684/69‑1 (MQ=255) cGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGatat < 1:2687363/69‑1 (MQ=255) cGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGatat < 1:2218205/69‑1 (MQ=255) cGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGatat < 1:2404811/69‑1 (MQ=255) cGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGatat < 1:3035739/69‑1 (MQ=255) cGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGatat < 1:3297129/69‑1 (MQ=255) cGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGatat < 1:751265/69‑1 (MQ=255) cGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGatat < 1:942153/69‑1 (MQ=255) gagacgacGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATAtgg > 1:1840785/3‑69 (MQ=255) gCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATAtgg > 1:3206086/1‑69 (MQ=255) gCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATAtgg > 1:1241696/1‑69 (MQ=255) cgacgGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATAtggtgtt < 1:2597738/69‑3 (MQ=255) cgacgGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATggtggt < 1:3253579/69‑1 (MQ=255) cgacgGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATggtggt < 1:3385072/69‑1 (MQ=255) cgacgGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATggtggt < 1:432525/69‑1 (MQ=255) cgacgGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATggtggt < 1:443557/69‑1 (MQ=255) cgacgGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATggtggt < 1:993266/69‑1 (MQ=255) cgacgGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATggtggt < 1:1983430/69‑1 (MQ=255) cgacgGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATggtggt < 1:2375812/69‑1 (MQ=255) cgacgGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATggtggt < 1:2088922/69‑1 (MQ=255) cgacgGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATggtggt < 1:1793159/69‑1 (MQ=255) cgacgGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATggtggt < 1:1764664/69‑1 (MQ=255) cgacgGTTTACACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATggtggt < 1:3273315/69‑1 (MQ=255) gacgGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATggtggt < 1:1197155/68‑1 (MQ=255) gacgGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATggtggt < 1:3389038/68‑1 (MQ=255) acgGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATggtggt < 1:1392959/67‑1 (MQ=255) gTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATGGTGGTAACTa < 1:1740759/69‑1 (MQ=255) gTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATGGTGGTAACTa < 1:368653/69‑1 (MQ=255) gTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATGGTGGTAACTa < 1:2133610/69‑1 (MQ=255) gTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATGGTGGTAACTa < 1:1608405/69‑1 (MQ=255) gTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATGGTGGTAACTa < 1:1359086/69‑1 (MQ=255) gTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATGGTGGTAACTa < 1:3028361/69‑1 (MQ=255) tttCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATGGTGGTAACTa < 1:2332294/68‑1 (MQ=255) tttCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATGGTGGTAACTAc < 1:2181328/69‑1 (MQ=255) tttCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATGGTGGTAACTAc < 1:473043/69‑1 (MQ=255) ttCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATAtggt > 1:3260447/1‑59 (MQ=255) ttCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATAtgg > 1:3424155/1‑58 (MQ=255) ttCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGgcgc > 1:1520591/1‑67 (MQ=255) ccAGAGAACGACCCTCAGCTGCCAGGGCACTTATATGGTGGTAACTACCGTACATTGCCGGGCGCATc < 1:2428748/68‑1 (MQ=255) ccAGAGAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAAGTACCGTACATTGCCGGGCGCATc < 1:2859948/68‑1 (MQ=255) ccAGAGAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATc < 1:1688774/68‑1 (MQ=255) ccAGAGAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATc < 1:2048698/68‑1 (MQ=255) ccAGAGAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATc < 1:1975294/68‑1 (MQ=255) ccAGAGAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATc < 1:360890/68‑1 (MQ=255) ccAGAGAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATc < 1:2113973/68‑1 (MQ=255) cAGAGAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATc < 1:3238559/67‑1 (MQ=255) agagAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGgcgc > 1:2639999/1‑63 (MQ=255) agagAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGgcgc > 1:2761489/1‑63 (MQ=255) agagAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGgcgc > 1:1572206/1‑63 (MQ=255) agagAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGgcgc > 1:154959/1‑63 (MQ=255) agagAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGgcgc > 1:745933/1‑63 (MQ=255) agagAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGgcgc > 1:2597879/1‑63 (MQ=255) agagAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGgcgc > 1:846741/1‑63 (MQ=255) agagAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGgcgc > 1:905154/1‑63 (MQ=255) agagAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGgcgc > 1:135712/1‑63 (MQ=255) agagAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGgcgc > 1:244641/1‑63 (MQ=255) agagAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGgcgc > 1:458599/1‑63 (MQ=255) agagAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGgcgc > 1:2660923/1‑63 (MQ=255) agagAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGgcgc > 1:343608/1‑63 (MQ=255) agagAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGgcgc > 1:3297085/1‑63 (MQ=255) agagAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGgcgc > 1:3009881/1‑63 (MQ=255) agagAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGgcgc > 1:322893/1‑63 (MQ=255) agagAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGgcgc > 1:3207924/1‑63 (MQ=255) agagAACGACCCTCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGgcgc > 1:2022126/1‑63 (MQ=255) ataaCGACCATCAGCTGCCAGGGCACTGATATGGTGGTAACTAc < 1:2261858/42‑1 (MQ=255) aCCATCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGaa < 1:2951328/69‑1 (MQ=255) aCCATCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGaa < 1:2448006/69‑1 (MQ=255) aCCATCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGaa < 1:3033557/69‑1 (MQ=255) | TCACATAACGGTCCGGCGACGACGGTTTCCACCGTTGGCGCGTGTTCCAGAGAACGACCATCAGCTGCCAGGGCACTGATATGGTGGTAACTACCGTACATTGCCGGGCGCATCAGATCGTTGAA > minE/1924909‑1925033 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |