| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,926,225 | G→A | 65.6% | G56G (GGG→GGA) | lysR → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,926,225 | 0 | G | A | 65.6% | 62.2 / 51.5 | 90 | G56G (GGG→GGA) | lysR | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base G (11/20); new base A (38/21); total (49/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.38e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 | |||||||||||
GTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGG > minE/1926160‑1926282 | gTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCg > 1:1056198/1‑68 (MQ=255)gTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCg > 1:1080474/1‑68 (MQ=255)gTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCg > 1:3310460/1‑68 (MQ=255)gTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCg > 1:2054584/1‑68 (MQ=255)gTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCg > 1:1561928/1‑68 (MQ=255) cAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGAt > 1:391255/1‑68 (MQ=255) cAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGAt > 1:1658232/1‑68 (MQ=255) cAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGAt > 1:885075/1‑68 (MQ=255) ccGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTAc < 1:335243/68‑1 (MQ=255) ccGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACa < 1:77723/69‑1 (MQ=255) cgcgAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACAt < 1:987488/69‑1 (MQ=255) cgcgAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACAt > 1:1216001/1‑69 (MQ=255) gcgAACTTGCGCGCTTTGAGAAGGTGATCTGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACAt < 1:3080090/68‑1 (MQ=255) gcgAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACAt < 1:2274830/68‑1 (MQ=255) tggcgctcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGAtt < 1:2456249/56‑1 (MQ=255) tggcgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGAtt < 1:702964/56‑1 (MQ=255) tggcgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGAtt < 1:513153/56‑1 (MQ=255) tggcgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGAtt < 1:448398/56‑1 (MQ=255) tggcgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGAtt < 1:2167019/56‑1 (MQ=255) tggcgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGAtt < 1:69235/56‑1 (MQ=255) tggcgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGAtt < 1:945830/56‑1 (MQ=255) tggcgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGAtt < 1:700926/56‑1 (MQ=255) tggcgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGAtt < 1:1440642/56‑1 (MQ=255) tggcgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGAtt < 1:2527831/56‑1 (MQ=255) tggcgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGAtt < 1:2532581/56‑1 (MQ=255) tggcgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGAtt < 1:1734373/56‑1 (MQ=255) tggcgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGAtt < 1:1705307/56‑1 (MQ=255) tggcgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGAtt < 1:1142609/56‑1 (MQ=255) tggcgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGAtt < 1:1452194/56‑1 (MQ=255) tggcgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGAtt < 1:280233/56‑1 (MQ=255) tggcgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGAtt < 1:3009250/56‑1 (MQ=255) tggcgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGAt < 1:2717935/55‑1 (MQ=255) tggcgcgcTTTGAGAAGGTGATCGGGCTGAAATTG‑TTGAGCGCATACGTGGACGAtt < 1:3076157/55‑1 (MQ=255) ggcgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGAtt < 1:642743/56‑1 (MQ=255) gcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAg < 1:1508491/69‑1 (MQ=255) gcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAg < 1:373279/69‑1 (MQ=255) gcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAg < 1:1317028/69‑1 (MQ=255) gcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAg < 1:1497441/69‑1 (MQ=255) cTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAg < 1:3022359/68‑1 (MQ=255) cTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAg < 1:1512357/68‑1 (MQ=255) cTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAg < 1:1636784/68‑1 (MQ=255) cTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAg < 1:2726996/68‑1 (MQ=255) ttGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGAtt < 1:325286/49‑1 (MQ=39) tGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAg > 1:3264517/1‑65 (MQ=255) tGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACt > 1:2320828/1‑69 (MQ=255) tGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACt > 1:3145605/1‑69 (MQ=255) tGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACt > 1:3050184/1‑69 (MQ=255) tGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACt > 1:3048806/1‑69 (MQ=255) tGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACt > 1:149535/1‑69 (MQ=255) tGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACt > 1:3223679/1‑69 (MQ=255) tGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACt > 1:2775312/1‑69 (MQ=255) tGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACt > 1:3307109/1‑69 (MQ=255) tGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACt > 1:904886/1‑69 (MQ=255) tGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACt > 1:2608789/1‑69 (MQ=255) tGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACt > 1:1293555/1‑69 (MQ=255) tGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACt > 1:849064/1‑69 (MQ=255) tGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACt > 1:25324/1‑69 (MQ=255) tGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACt > 1:187868/1‑69 (MQ=255) tGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACt > 1:2524650/1‑69 (MQ=255) tGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACt > 1:2066875/1‑69 (MQ=255) tGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACt > 1:2498113/1‑69 (MQ=255) tGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACt > 1:617717/1‑69 (MQ=255) tGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTg < 1:1062775/68‑1 (MQ=255) tGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTg < 1:2746768/68‑1 (MQ=255) tGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTg < 1:1782623/68‑1 (MQ=255) tGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTg < 1:2305661/68‑1 (MQ=255) tGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTg < 1:2346847/68‑1 (MQ=255) gggCTGGAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTga > 1:437693/1‑68 (MQ=255) gggCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGt > 1:2466603/1‑47 (MQ=39) gggCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTga > 1:2417679/1‑68 (MQ=255) gggCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTga > 1:1121565/1‑68 (MQ=255) gggCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTga > 1:1401018/1‑68 (MQ=255) gggCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTga > 1:1444412/1‑68 (MQ=255) gggCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTga > 1:155363/1‑68 (MQ=255) gggCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTga > 1:1889476/1‑68 (MQ=255) gggCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTga > 1:735632/1‑68 (MQ=255) gggCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTga > 1:1970789/1‑68 (MQ=255) gggCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTga > 1:2017561/1‑68 (MQ=255) gggCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTga > 1:3066329/1‑68 (MQ=255) gggCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTga > 1:250551/1‑68 (MQ=255) gggCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTga > 1:3317154/1‑68 (MQ=255) gggCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTga > 1:2630477/1‑68 (MQ=255) gggCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTga > 1:2706575/1‑68 (MQ=255) gggCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTga > 1:2728534/1‑68 (MQ=255) gggCTGAAATTGTTTGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTga > 1:3180815/1‑68 (MQ=255) gggCTGAAATTGTATGAGCGCATACGTGGACGATTACATCCTACCGTGCAAGGACTGCGTCTg > 1:626387/1‑63 (MQ=255) gTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACg > 1:210690/1‑69 (MQ=255) gTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACg > 1:2473669/1‑69 (MQ=255) cgcgTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTgg < 1:2583304/69‑1 (MQ=255) cgcgTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTgg < 1:2789735/69‑1 (MQ=255) | GTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGG > minE/1926160‑1926282 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |