| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 141,333 | A→T | 35.8% | A97A (GCT→GCA) | yadN ← | predicted fimbrial‑like adhesin protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 141,333 | 0 | A | T | 35.8% | 56.5 / 52.0 | 67 | A97A (GCT→GCA) | yadN | predicted fimbrial‑like adhesin protein |
| Reads supporting (aligned to +/- strand): ref base A (31/12); new base T (9/15); total (40/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.02e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTA > minE/141267‑141399 | aaTAGACATGTCGTTGTTCGGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:2416876/69‑1 (MQ=255)aaTAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:2979815/69‑1 (MQ=255)aaTAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:909803/69‑1 (MQ=255)aaTAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:754957/69‑1 (MQ=255)aaTAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:3066429/69‑1 (MQ=255)aaTAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:3116142/69‑1 (MQ=255)aaTAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:2021928/69‑1 (MQ=255)aaTAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:2914283/69‑1 (MQ=255)aaTAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:37950/69‑1 (MQ=255) aTAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:3060745/68‑1 (MQ=255) aTAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:379633/68‑1 (MQ=255) tAGACATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:3409462/67‑1 (MQ=255) cATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGCAGtagt > 1:1460798/1‑66 (MQ=255) cATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGCAGtagt > 1:2866839/1‑66 (MQ=255) cATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGCAGtagt > 1:747510/1‑66 (MQ=255) cATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGCAGtagt > 1:2139445/1‑66 (MQ=255) cATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGCAGtagt > 1:1381223/1‑66 (MQ=255) cATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGCAGtagt > 1:2931302/1‑66 (MQ=255) cATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGCAGtagt > 1:1545499/1‑66 (MQ=255) cATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGCAGtagt > 1:2886813/1‑66 (MQ=255) cATGTCGTTGTTCAGTGTTCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGCAGtagt > 1:143881/1‑66 (MQ=255) gTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTAcc > 1:1805546/1‑69 (MQ=255) gTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTAcc > 1:1353637/1‑69 (MQ=255) gTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTAcc > 1:355765/1‑69 (MQ=255) gTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTAcc > 1:1238580/1‑69 (MQ=255) tCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTAcc > 1:1686035/1‑68 (MQ=255) tCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTAcc > 1:677764/1‑68 (MQ=255) tCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTAcc > 1:3248453/1‑68 (MQ=255) tCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTAcc > 1:886182/1‑68 (MQ=255) cAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCgg < 1:1787470/69‑1 (MQ=255) aaGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGa < 1:1055308/68‑1 (MQ=255) ttccTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:2645817/44‑1 (MQ=255) tGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTTGc < 1:2855215/40‑1 (MQ=255) tGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGAtttgctttgc > 1:3128209/1‑69 (MQ=255) tGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGAtttgctttgc > 1:3265060/1‑69 (MQ=255) tGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGAtttgctttgc > 1:3300595/1‑69 (MQ=255) tGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGAtttgctttgc > 1:973231/1‑69 (MQ=255) tGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGAtttgctttgc > 1:1864451/1‑69 (MQ=255) aCCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTc > 1:1453195/1‑69 (MQ=255) aCCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTc > 1:2620124/1‑69 (MQ=255) aCCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTc > 1:1110365/1‑69 (MQ=255) aaaGAAAACAGAACCGAAGGTCATTTTTGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAAc < 1:1475025/69‑1 (MQ=255) aaaGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAAc < 1:1012957/69‑1 (MQ=255) aaaGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAAc < 1:2676475/69‑1 (MQ=255) aaaGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAAc < 1:2501006/69‑1 (MQ=255) aaaGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAAc < 1:2830622/69‑1 (MQ=255) aaaGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAAc < 1:1504164/69‑1 (MQ=255) aaGAAAACAGAACCAAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACg < 1:2355820/69‑1 (MQ=255) gAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGt < 1:676489/69‑1 (MQ=255) gAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGt < 1:1823208/69‑1 (MQ=255) gAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGt < 1:2681918/69‑1 (MQ=255) gAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACg < 1:1188512/55‑1 (MQ=255) cATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGtttagc > 1:726692/1‑69 (MQ=255) cATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGtttagc > 1:1347489/1‑69 (MQ=255) cATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGtttagc > 1:965616/1‑69 (MQ=255) cATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGtttagc > 1:1431730/1‑69 (MQ=255) cATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGtttagc > 1:150042/1‑69 (MQ=255) cATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGtttagc > 1:654914/1‑69 (MQ=255) cATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGtttagc > 1:1436203/1‑69 (MQ=255) cATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGtttagc > 1:1826776/1‑69 (MQ=255) cATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGtttagc > 1:2284005/1‑69 (MQ=255) cATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGtttagc > 1:2376603/1‑69 (MQ=255) cATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGtttagc > 1:2465029/1‑69 (MQ=255) ttAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTc > 1:511547/1‑41 (MQ=255) ttAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGtttagcttta > 1:1110248/1‑69 (MQ=255) ttAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGtttagcttta > 1:2511866/1‑69 (MQ=255) ttAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGtttagcttt > 1:2846256/1‑68 (MQ=255) | AATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTA > minE/141267‑141399 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |