| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,930,883 | G→A | 27.6% | G93G (GGC→GGT) | kduI ← | predicted 5‑keto 4‑deoxyuronate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,930,883 | 0 | G | A | 27.6% | 143.8 / 50.2 | 126 | G93G (GGC→GGT) | kduI | predicted 5‑keto 4‑deoxyuronate isomerase |
| Reads supporting (aligned to +/- strand): ref base G (39/52); new base A (14/21); total (53/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 126 total aligned reads displayed.
GACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGA > minE/1930820‑1930947 | gACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGGCATc > 1:31956/1‑69 (MQ=255)gACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATc > 1:2012326/1‑69 (MQ=255)gACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATc > 1:2245510/1‑69 (MQ=255)gACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATc > 1:2533422/1‑69 (MQ=255)gACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATc > 1:2948643/1‑69 (MQ=255) aCAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCg < 1:3364575/69‑1 (MQ=255) aCAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCg < 1:1364088/69‑1 (MQ=255) ttcttTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGACCATCGACAg > 1:637115/1‑68 (MQ=255) ttcttTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGACCATCGACAGt > 1:3022536/1‑69 (MQ=255) ttcttTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGACCATCGACAGt > 1:2131715/1‑69 (MQ=255) ttcttTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGACCATCGACAGt > 1:31884/1‑69 (MQ=255) ttcttTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGACCATCGACAGt > 1:1934253/1‑69 (MQ=255) ttcttTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGACCATCGACAGt > 1:2667552/1‑69 (MQ=255) ttcttTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGACCATCGACAGt > 1:1524845/1‑69 (MQ=255) ttcttTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGACCATCGACAGt > 1:1837419/1‑69 (MQ=255) ttcttTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGACCATCGACAGt > 1:2609724/1‑69 (MQ=255) ttcttTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGACCATCGACAGt > 1:1805717/1‑69 (MQ=255) gCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCg < 1:2829989/67‑1 (MQ=255) gCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCg < 1:1379570/67‑1 (MQ=255) gCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCg < 1:907227/67‑1 (MQ=255) gCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCg < 1:897997/67‑1 (MQ=255) gCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCg < 1:2956304/67‑1 (MQ=255) gCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGt < 1:57249/68‑1 (MQ=255) gCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGt < 1:180125/68‑1 (MQ=255) gCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGt < 1:2629432/68‑1 (MQ=255) gCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGt < 1:2733577/68‑1 (MQ=255) cATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCAGCGCCaa < 1:4575/69‑1 (MQ=255) cATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCaa < 1:2468699/69‑1 (MQ=255) cATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCaa > 1:2440956/1‑69 (MQ=255) cATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCaa < 1:2659997/69‑1 (MQ=255) cATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCaa < 1:223053/69‑1 (MQ=255) cATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCaa < 1:460651/69‑1 (MQ=255) cATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCaa < 1:2067700/69‑1 (MQ=255) cATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCaa < 1:2036939/69‑1 (MQ=255) cATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCaa > 1:1082533/1‑69 (MQ=255) cATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCaa < 1:1984418/69‑1 (MQ=255) cATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCaa < 1:1326633/69‑1 (MQ=255) cATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCaa < 1:1574260/69‑1 (MQ=255) cATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCaa < 1:835236/69‑1 (MQ=255) cATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCaa < 1:1697550/69‑1 (MQ=255) cATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCaa < 1:1844523/69‑1 (MQ=255) tACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCTACAGTAATCGTACCGGCACCGCCAata > 1:822956/1‑69 (MQ=255) tACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTAcc > 1:543128/1‑56 (MQ=255) tACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCCCCGCCAat > 1:3334789/1‑68 (MQ=255) tACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCaa > 1:2981750/1‑67 (MQ=255) tACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAata > 1:618552/1‑69 (MQ=255) tACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAata > 1:3034649/1‑69 (MQ=255) tACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAata > 1:1836881/1‑69 (MQ=255) tACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAata > 1:2241129/1‑69 (MQ=255) tACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAata > 1:256483/1‑69 (MQ=255) tACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAata > 1:747755/1‑69 (MQ=255) tACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAata > 1:1351921/1‑69 (MQ=255) tACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAata > 1:464255/1‑69 (MQ=255) tACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAat > 1:1198918/1‑68 (MQ=255) cAGGGCGTCGCGGTGACCGATTTCATAGCATTGGcc > 1:2208692/1‑36 (MQ=255) cAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCg > 1:3032042/1‑50 (MQ=255) cAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCg > 1:384408/1‑50 (MQ=255) cAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCg > 1:663284/1‑50 (MQ=255) cAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCg > 1:1926893/1‑50 (MQ=255) cAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATAtt > 1:1504372/1‑69 (MQ=255) cAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATAtt > 1:1185910/1‑69 (MQ=255) cAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATAtt > 1:3001021/1‑69 (MQ=255) ggCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGAt < 1:2448933/69‑1 (MQ=255) ggCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGAt < 1:385885/69‑1 (MQ=255) ggCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGAt > 1:3235595/1‑69 (MQ=255) ggCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGAt < 1:3226750/69‑1 (MQ=255) ggCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGAt < 1:1197388/69‑1 (MQ=255) ggCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGAt < 1:1724968/69‑1 (MQ=255) ggCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGAt < 1:403721/69‑1 (MQ=255) ggCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGAt < 1:564159/69‑1 (MQ=255) ggCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGAt < 1:467097/69‑1 (MQ=255) ggCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGAt > 1:80365/1‑69 (MQ=255) gCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCaa < 1:2928745/61‑1 (MQ=255) cGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCaa < 1:1311145/60‑1 (MQ=255) gTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAac < 1:1491722/69‑1 (MQ=255) gTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAac < 1:1949316/69‑1 (MQ=255) gcgGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAacac > 1:3309293/1‑68 (MQ=255) aCCGATTTCATAGCATTGATCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTc < 1:1206480/69‑1 (MQ=255) aCCGATTTCATAGCATTGACCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTc < 1:908039/69‑1 (MQ=255) aCCGATTTCATAGCATTGACCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTc < 1:2990177/69‑1 (MQ=255) aCCGATTTCATAGCATTGACCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTc < 1:1158430/69‑1 (MQ=255) aCCGATTTCATAGCATTGACCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTc < 1:1268032/69‑1 (MQ=255) aCCGATTTCATAGCATTGACCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTc < 1:1546990/69‑1 (MQ=255) aCCGATTTCATAGCATTGACCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTc < 1:2400020/69‑1 (MQ=255) aCCGATTTCATAGCATTGACCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTc < 1:2498875/69‑1 (MQ=255) aCCGATTTCATAGCATTGACCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTc < 1:252239/69‑1 (MQ=255) aCCGATTTCATAGCATTGACCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTc < 1:2590175/69‑1 (MQ=255) aCCGATTTCATAGCATTGACCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTc < 1:2665778/69‑1 (MQ=255) aCCGATTTCATAGCATTGACCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTc < 1:2837877/69‑1 (MQ=255) aCCGATTTCATAGCATTGACCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTc < 1:3410717/69‑1 (MQ=255) ccGATTTCATAGCATTGACCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTc < 1:2897383/68‑1 (MQ=255) ccGATTTCATAGCATTGACCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTc < 1:1162829/68‑1 (MQ=255) ccGATTTCATAGCATTGACCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTc < 1:1148508/68‑1 (MQ=255) cGATTTCATAGCATTGACCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTc < 1:1931408/67‑1 (MQ=255) gATTTCATAGCATTGACCATCGACAGTATTCGTACCGGCACCGCCAATATTGATAACACCTAACTc < 1:1937/66‑1 (MQ=255) tttCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGAt < 1:1990405/52‑1 (MQ=255) tttCATAGCATTGACCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTc < 1:550513/64‑1 (MQ=255) tAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCaa < 1:1411227/40‑1 (MQ=255) aTTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGa < 1:2508637/69‑1 (MQ=255) aTTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGa < 1:677199/69‑1 (MQ=255) | GACAACTTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGA > minE/1930820‑1930947 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |