| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,932,021 | C→T | 37.2% | M203I (ATG→ATA) | yqeF ← | predicted acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,932,021 | 0 | C | T | 37.2% | 35.1 / 33.8 | 59 | M203I (ATG→ATA) | yqeF | predicted acyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (26/11); new base T (7/15); total (33/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.42e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCT > minE/1931970‑1932089 | gCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGAtctc < 1:3199079/69‑1 (MQ=255)gCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGAtctc > 1:2349006/1‑69 (MQ=255)gCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGAtctc > 1:250401/1‑69 (MQ=255)gCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGAtctc > 1:427459/1‑69 (MQ=255)gCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGAtctc < 1:747990/69‑1 (MQ=255)gCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGAtctc > 1:151746/1‑69 (MQ=255)gCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGAtctc > 1:39248/1‑69 (MQ=255)gCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGAtctc > 1:1722490/1‑69 (MQ=255)gCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGAtct > 1:2178196/1‑68 (MQ=255)gCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGAtct > 1:134904/1‑68 (MQ=255) tGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATc > 1:2154103/1‑69 (MQ=255) cATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATc > 1:2300573/1‑69 (MQ=255) cATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATc > 1:2211021/1‑69 (MQ=255) cATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATc > 1:3418983/1‑69 (MQ=255) cATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATc > 1:2762128/1‑69 (MQ=255) cATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATc > 1:3071522/1‑69 (MQ=255) cATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATc > 1:923720/1‑69 (MQ=255) tCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCg < 1:2246271/68‑1 (MQ=255) cGGTATCAACAACCAACGTCTGTCCGTTACTTTGGGTTATTACCGGGACg < 1:2682106/50‑1 (MQ=39) cGGTATCAACAACCAACGTCTGTCCGTTACTTTGGGTTATTACCGGGACg < 1:262510/50‑1 (MQ=39) cGGTATCAACAACCAACGTCTGTCCGTTACTTTGGGTTATTACCGGGACg < 1:2551178/50‑1 (MQ=39) cGGTATCAACAACCAACGTCTGTCCGTTACTTTGGGTTATTACCGGGACg < 1:2866277/50‑1 (MQ=39) cGGTATCAACAACCAACGTCTGTCCGTTACTTTGGGTTATTACCGGGACg < 1:3364621/50‑1 (MQ=39) cGGTATCAACAACCAACGTCTGTCCGTTACTTTGGGTTATTACCGGGACg < 1:2316980/50‑1 (MQ=39) cGGTATCAACAACCAACGTCTGTCCGTTACTTTGGGTTATTACCGGGACg < 1:3308660/50‑1 (MQ=39) cGGTATCAACAACCAACGTCTGTCCGTTACTTTGGGTTATTACCGGGACg < 1:1364265/50‑1 (MQ=39) cGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTc > 1:3373029/1‑69 (MQ=255) cGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTc > 1:746652/1‑69 (MQ=255) gTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCg > 1:1343158/1‑69 (MQ=255) gTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCg > 1:568748/1‑69 (MQ=255) aacaacCAACGTCTGCCCGTTACTTTGGGTCATTa > 1:2326321/1‑35 (MQ=255) aacaacCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCTTc > 1:1357229/1‑69 (MQ=255) aacaacCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGt > 1:769913/1‑68 (MQ=255) aacaacCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTc > 1:482295/1‑69 (MQ=255) aacaacCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTc > 1:748529/1‑69 (MQ=255) aacaacCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTc > 1:1832502/1‑69 (MQ=255) aacaacCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTc > 1:2390645/1‑69 (MQ=255) caaccaacGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCa < 1:2684635/68‑1 (MQ=255) caaccaacGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCa < 1:2114381/68‑1 (MQ=255) caaccaacGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCa < 1:2123779/68‑1 (MQ=255) caaccaacGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCa < 1:454802/68‑1 (MQ=255) caaccaacGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCa < 1:860766/68‑1 (MQ=255) aacGTCTGTCCGTTACTTTGGGTTATTACCGGGACg < 1:1298125/36‑1 (MQ=37) tCTGTCCGTTACTTTGGGTTATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATcgccgc > 1:778704/1‑67 (MQ=255) tCTGTCCGTTACTTTGGGTTATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATcgccgc > 1:1151132/1‑67 (MQ=255) tCTGTCCGTTACTTTGGGTTATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATcgccgc > 1:3246543/1‑67 (MQ=255) tCTGTCCGTTACTTTGGGTTATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATcgccgc > 1:1233664/1‑67 (MQ=255) tCTGTCCGTTACTTTGGGTTATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATcgccgc > 1:257162/1‑67 (MQ=255) tCTGTCCGTTACTTTGGGTTATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATcgccgc > 1:1478944/1‑67 (MQ=255) tCTGTCCGTTACTTTGGGTTATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATcgccgc > 1:1599303/1‑67 (MQ=255) cGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCt < 1:2731148/68‑1 (MQ=255) cGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCt < 1:1773729/68‑1 (MQ=255) tttGGGTTATTACCGGGACGATCTCATCTTTAAATGGTCCGGCGTCAATCGCCGCTCGCGCTTTttgt < 1:2757249/68‑1 (MQ=255) tttGGGTTATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTttgt < 1:3138924/68‑1 (MQ=255) tttGGGTTATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTttgt < 1:731928/68‑1 (MQ=255) tttGGGTTATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTttgt < 1:652886/68‑1 (MQ=255) tttGGGTTATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTttgt < 1:204381/68‑1 (MQ=255) tttGGGTTATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTttgt < 1:2671829/68‑1 (MQ=255) ggTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCg < 1:1043784/68‑1 (MQ=255) cATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCt < 1:1763255/69‑1 (MQ=255) cATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCt < 1:222273/69‑1 (MQ=255) cATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCt < 1:2272201/69‑1 (MQ=255) cATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCt < 1:141321/69‑1 (MQ=255) cATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCt < 1:963143/69‑1 (MQ=255) | GCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCT > minE/1931970‑1932089 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |