| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,932,207 | G→A | 31.1% | D141D (GAC→GAT) | yqeF ← | predicted acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,932,207 | 0 | G | A | 31.1% | 121.5 / 62.6 | 128 | D141D (GAC→GAT) | yqeF | predicted acyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (63/25); new base A (19/21); total (82/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.03e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01 | |||||||||||
Only 100 of 128 total aligned reads displayed.
ATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACATGTGGTGCGCG > minE/1932141‑1932272 | aTTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTATc < 1:2375423/69‑1 (MQ=255)aTTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTATc < 1:1889772/69‑1 (MQ=255)aTTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTATc < 1:3057567/69‑1 (MQ=255)aTTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTATc < 1:3061197/69‑1 (MQ=255)aTTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTATc < 1:1802992/69‑1 (MQ=255)aTTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTATc < 1:3108552/69‑1 (MQ=255)aTTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTATc < 1:1664612/69‑1 (MQ=255)aTTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTATc < 1:314517/69‑1 (MQ=255)aTTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTATc < 1:3187658/69‑1 (MQ=255)aTTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTATc < 1:356680/69‑1 (MQ=255)aTTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTATc < 1:142161/69‑1 (MQ=255)aTTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTATc < 1:2626060/69‑1 (MQ=255)aTTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTATc < 1:130647/69‑1 (MQ=255)aTTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTATc < 1:2391514/69‑1 (MQ=255)aTTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTATc < 1:478307/69‑1 (MQ=255)aTTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTATc < 1:475833/69‑1 (MQ=255)aTTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCACACAACCCATAATGCACAAGACTATc < 1:3174929/69‑1 (MQ=255) ggcggTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaacca < 1:2553251/68‑1 (MQ=255) ggcggTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaacca < 1:2845843/68‑1 (MQ=255) ggcggTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaacca < 1:668813/68‑1 (MQ=255) ggcggTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaacca < 1:1150170/68‑1 (MQ=255) ggcggTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaacca < 1:312881/68‑1 (MQ=255) ggcggTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaacca < 1:2543224/68‑1 (MQ=255) ggcggTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaacca < 1:1586788/68‑1 (MQ=255) ggcggTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaacca < 1:3241209/68‑1 (MQ=255) ggcggTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaacca < 1:1493596/68‑1 (MQ=255) ggcggTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaacca < 1:2649524/68‑1 (MQ=255) ggcggTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaacca < 1:3367182/68‑1 (MQ=255) cggTTACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTATc < 1:2689061/61‑1 (MQ=255) aTAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTgg > 1:1677136/1‑55 (MQ=255) aTAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGc > 1:2176401/1‑56 (MQ=255) aTAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGc > 1:2366528/1‑56 (MQ=255) aTAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGc > 1:395387/1‑56 (MQ=255) aTAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGc > 1:1298596/1‑56 (MQ=255) aTAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGc > 1:1154466/1‑56 (MQ=255) aTTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCtgtg > 1:1598044/1‑65 (MQ=255) aTTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGc < 1:2881355/66‑1 (MQ=255) aTTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGc > 1:2718852/1‑66 (MQ=255) aTTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGc < 1:2710370/66‑1 (MQ=255) aTTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGc < 1:329537/66‑1 (MQ=255) aTTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGc > 1:362515/1‑66 (MQ=255) aTTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGc > 1:66232/1‑66 (MQ=255) aTTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGc > 1:97078/1‑66 (MQ=255) aTTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGc < 1:2506034/66‑1 (MQ=255) aTTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGc > 1:1835253/1‑66 (MQ=255) aTTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGc > 1:1879325/1‑66 (MQ=255) aTTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGc > 1:2232310/1‑66 (MQ=255) aTTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGc > 1:1928338/1‑66 (MQ=255) aTTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGc < 1:1042489/66‑1 (MQ=255) aTTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCAAAg > 1:2059450/1‑60 (MQ=255) aTTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACAAACTGGCTATTGCCAAGCtg > 1:1601934/1‑63 (MQ=255) gAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGt > 1:1465548/1‑69 (MQ=255) gAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGt > 1:2550454/1‑69 (MQ=255) gAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGt > 1:1132177/1‑69 (MQ=255) gAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGt > 1:2067484/1‑69 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtc > 1:415673/1‑69 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtc > 1:1377303/1‑69 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtc > 1:840871/1‑69 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtc > 1:420671/1‑69 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtc > 1:1163811/1‑69 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtc > 1:2466589/1‑69 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtc > 1:2405195/1‑69 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtc > 1:2260869/1‑69 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACAAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtc > 1:1885313/1‑69 (MQ=255) ccACAACCCATCATCCACAAGACTGTCAACCAACTGCCTAt > 1:1796323/1‑41 (MQ=38) caACCCCTCATGCACAAGACTATCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtca > 1:3999/1‑67 (MQ=255) caACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtca > 1:2830759/1‑67 (MQ=255) caACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtca > 1:1493016/1‑67 (MQ=255) caACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtca > 1:1430663/1‑67 (MQ=255) caACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtca > 1:3253715/1‑67 (MQ=255) caACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtca > 1:2996779/1‑67 (MQ=255) caACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtca > 1:48090/1‑67 (MQ=255) caACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtca > 1:2228118/1‑67 (MQ=255) caACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtca > 1:1020548/1‑67 (MQ=255) aaCCCATCATGCACAAGACTATCAACCTACTGGCTATTGCCAAGCTGTGCGCCGGt > 1:588843/1‑56 (MQ=255) aaCCCATCATGCACAAGACTATCAACCAACTGGCTATTGCCAAGCTGTGCGCCGGt > 1:1318673/1‑56 (MQ=255) aaCCCATCATGCACAAGACTATCAACCAACTGGCTATTGCCAAGCTGTGCGCCGGt > 1:620523/1‑56 (MQ=255) aaCCCATCATGCACAAGACTATCAACCAACTGGCTATTGCCAAGCTGTGCGCCGGt > 1:34464/1‑56 (MQ=255) aaCCCATCATGCACAAGACTATCAACCAACTGGCTATTGCCAAGCTGTGCGCCGGt > 1:3368303/1‑56 (MQ=255) aaCCCATCATGCACAAGACTATCAACCAACTGGCTATTGCCAAGCTGTGCGCCGGt > 1:1497417/1‑56 (MQ=255) aaCCCATCATGCACAAGACTATCAACCAACTGGCTATTGCCAAGCTGTGCGCCGGt > 1:1555699/1‑56 (MQ=255) aaCCCATCATGCACAAGACTATCAACCAACTGGCTATTGCCAAGCTGTGCGCCGGt > 1:3062949/1‑56 (MQ=255) aaCCCATCATGCACAAGACTATCAACCAACTGGCTATTGCCAAGCTGTGCGCCGGt > 1:889360/1‑56 (MQ=255) aaCCCATCATGCACAAGACTATCAACCAACTGGCTATTGCCAAGCTGTGCGCCGGt > 1:2736835/1‑56 (MQ=255) aaCCCATCATGCACAAGACTATCAACCAACTGGCTATTGCCAAGCTGTGCGCCGGt > 1:2070222/1‑56 (MQ=255) aaCCCATCATGCACAAGACTATCAACCAACTGGCTATTGCCAAGCTGTGCGCCGGt > 1:2127979/1‑56 (MQ=255) aaCCCATCATGCACAAGACTATCAACCAACTGGCTATTGCCAAGCTGTGCGCCGGt > 1:2607865/1‑56 (MQ=255) aaCCCATCATGCACAAGACTATCAACCAACTGGCTATTGCCAAGCTGTGCGCCGGt > 1:2351238/1‑56 (MQ=255) aaCCCATCATGCACAAGACTATCAACCAACTGGCTATTGCCAAGCTGTGCGCCGGt > 1:2347218/1‑56 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcagtcag > 1:1101517/1‑63 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcagtcag > 1:1661313/1‑63 (MQ=255) gCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtgtg > 1:2132774/1‑69 (MQ=255) cacaAGACTGTCAACCAACTGTCTATTGCCGAGCTGTGCACCGGTGCGGCTATCAGTCAGAACATgtgg < 1:3049532/69‑1 (MQ=255) aCTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACATGTGGTgcgc > 1:3388482/1‑68 (MQ=255) aCTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACATGTGGTGcgcg > 1:1039429/1‑69 (MQ=255) aCTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACATGTGGTGcgcg > 1:3253581/1‑69 (MQ=255) aCTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACATGTGGTGcgcg > 1:2975928/1‑69 (MQ=255) aCTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACATGTGGTGcgcg > 1:2888652/1‑69 (MQ=255) aCTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACATGTGGTGcgcg > 1:2170787/1‑69 (MQ=255) cTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGGGCGGCTATCAGTCAGAACATgtgg < 1:1747730/62‑1 (MQ=255) | ATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACATGTGGTGCGCG > minE/1932141‑1932272 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |