| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 147,876 | C→T | 29.6% | A344A (GCC→GCT) | hrpB → | predicted ATP‑dependent helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 147,876 | 0 | C | T | 29.6% | 62.7 / 26.9 | 64 | A344A (GCC→GCT) | hrpB | predicted ATP‑dependent helicase |
| Reads supporting (aligned to +/- strand): ref base C (22/23); new base T (10/9); total (32/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
TCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATGGAAT > minE/147822‑147934 | tCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGa < 1:196224/69‑1 (MQ=255) cTGGAGCCGGGTATCTGCCTTCATTTAATCGCCAAAGAACAAGCAGAACGCGCTGCCGCGCAAAGTg > 1:1105008/1‑67 (MQ=255) cTGGAGCCGGGTATCTGCCTTCATTTAATCGCCAAAGAACAAGCAGAACGCGCTGCCGCGCAAAGTg > 1:2817785/1‑67 (MQ=255) cTGGAGCCGGGTATCTGCCTTCATTTAATCGCCAAAGAACAAGCAGAACGCGCTGCCGCGCAAAGTg > 1:1576222/1‑67 (MQ=255) cTGGAGCCGGGTATCTGCCTTCATTTAATCGCCAAAGAACAAGCAGAACGCGCTGCCGCGCAAAGTg > 1:662494/1‑67 (MQ=255) cTGGAGCCGGGTATCTGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCTGCCGCGCAAAGTg > 1:3088000/1‑67 (MQ=255) cTGGAGCCGGGTATCTGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCTGCCGCGCAAAGTg > 1:200945/1‑67 (MQ=255) cTGGAGCCGGGTATCTGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCTGCCGCGCAAAGTg > 1:22536/1‑67 (MQ=255) cTGGAGCCGGGTATCTGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCTGCCGCGCAAAGTg > 1:3055889/1‑67 (MQ=255) cTGGAGCCGGGTATCTGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCTGCCGCGCAAAGTg > 1:1418568/1‑67 (MQ=255) cTGGAGCCGGGTATCTGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCTGCCGCGCAAAGTg > 1:1203729/1‑67 (MQ=255) cGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGgaga < 1:616759/69‑1 (MQ=255) cGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGgaga < 1:2874394/69‑1 (MQ=255) cGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGgaga < 1:984877/69‑1 (MQ=255) aGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCTCAAAGTGAACCGGAGATCtt > 1:579749/1‑65 (MQ=255) aGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCtt > 1:7675/1‑65 (MQ=255) aGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCtt > 1:2493771/1‑65 (MQ=255) aGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCtt > 1:17961/1‑65 (MQ=255) aGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACaa > 1:1183566/1‑69 (MQ=255) aGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACaa > 1:2210875/1‑69 (MQ=255) aGCCTGAATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCg > 1:6911/1‑57 (MQ=255) tGCATTTAATCGCCAAAGAACAAGCAGAACGCGCTGCCGCGCAAAGTGAACCGGAGATCTTACAAAg < 1:2544047/67‑1 (MQ=255) tGCATTTAATCGCCAAAGAACAAGCAGAACGCGCTGCCGCGCAAAGTGAACCGGAGATCTTACAAAg < 1:861589/67‑1 (MQ=255) tGCATTTAATCGCCAAAGAACAAGCAGAACGCGCTGCCGCGCAAAGTGAACCGGAGATCTTACAAAg < 1:2142046/67‑1 (MQ=255) tGCATTTAATCGCCAAAGAACAAGCAGAACGCGCTGCCGCGCAAAGTGAACCGGAGATCTTACAAAg < 1:2023367/67‑1 (MQ=255) tGCATTTAATCGCCAAAGAACAAGCAGAACGCGCTGCCGCGCAAAGTGAACCGGAGATCTTACAAAg < 1:11256/67‑1 (MQ=255) tGCATTTAATCGCCAAAGAACAAGCAGAACGCGCTGCCGCGCAAAGTGAACCGGAGATCTTACAAAg < 1:1322127/67‑1 (MQ=255) tGCATTTAATCGCCAAAGAACAAGCAGAACGCGCTGCCGCGCAAAGTGAACCGGAGATCTTACAAAg < 1:1386991/67‑1 (MQ=255) tGCATTTAATCGCCAAAGAACAAGCAGAACGCGCTGCCGCGAAAAGTGAACCGGAGATCTTACAAAg < 1:687153/67‑1 (MQ=255) gCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCg < 1:3098256/68‑1 (MQ=255) cATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGAt > 1:1990316/1‑69 (MQ=255) cATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGAt > 1:893442/1‑69 (MQ=255) cATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGAt > 1:1938597/1‑69 (MQ=255) cATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGAt > 1:2600774/1‑69 (MQ=255) cATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGAt > 1:2654182/1‑69 (MQ=255) cATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGAt > 1:1423584/1‑69 (MQ=255) cATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGAt > 1:1739089/1‑69 (MQ=255) cATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGAt > 1:1736260/1‑69 (MQ=255) tAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCttt > 1:1104293/1‑69 (MQ=255) tAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCttt > 1:459864/1‑69 (MQ=255) cGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCgg < 1:202868/69‑1 (MQ=255) cGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCgg < 1:2192783/69‑1 (MQ=255) cGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCgg < 1:879705/69‑1 (MQ=255) gCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCgg > 1:2913918/1‑68 (MQ=255) aGAACAAGCAGAACGCGCTGCCGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCt < 1:3218178/69‑1 (MQ=255) aGAACAACCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAACCGATCTTTCCGGTTTGCt < 1:2707830/69‑1 (MQ=255) gAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTg > 1:2656772/1‑69 (MQ=255) gAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTg > 1:1512417/1‑69 (MQ=255) aGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATgg < 1:972994/68‑1 (MQ=255) cAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATgg < 1:2691489/66‑1 (MQ=255) cAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATgg < 1:3297983/66‑1 (MQ=255) cAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATgg < 1:598981/66‑1 (MQ=255) cAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATgg < 1:3107266/66‑1 (MQ=255) cAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATgg > 1:1742102/1‑66 (MQ=255) cAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATgg < 1:1375237/66‑1 (MQ=255) cAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATgg < 1:736424/66‑1 (MQ=255) cAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATgg < 1:2471930/66‑1 (MQ=255) cAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATgg > 1:1966119/1‑66 (MQ=255) cAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATgg < 1:1533246/66‑1 (MQ=255) cAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATgg < 1:1588845/66‑1 (MQ=255) cAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATGGAAt < 1:577806/69‑1 (MQ=255) cAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATGGAAt < 1:1035440/69‑1 (MQ=255) cAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATGGAAt < 1:925294/69‑1 (MQ=255) cAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATGGAAt < 1:2943021/69‑1 (MQ=255) | TCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATGGAAT > minE/147822‑147934 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |