| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,099 | G→A | 58.9% | D448D (GAC→GAT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,099 | 0 | G | A | 58.9% | 48.5 / 96.6 | 129 | D448D (GAC→GAT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (19/34); new base A (41/35); total (60/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.97e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
Only 100 of 133 total aligned reads displayed.
GGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAA > minE/1933031‑1933164 | ggcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCGCCTGCg > 1:2559879/1‑69 (MQ=255)ggcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCg > 1:1255179/1‑69 (MQ=255)ggcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCg > 1:1898338/1‑69 (MQ=255)ggcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCg > 1:497033/1‑69 (MQ=255) aaGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCttt > 1:646570/1‑68 (MQ=255) aaGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTc > 1:977689/1‑69 (MQ=255) aaGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTc > 1:3055794/1‑69 (MQ=255) aaGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTc > 1:2179617/1‑69 (MQ=255) aaGCCATGTTCCAGTGCGGTGACATAATCTTCGTCGTAGAACATCGCTTCGTCGTCACCTGCATCttt > 1:1026742/1‑68 (MQ=255) aaGCCATGTTCCAGTGCGGTGACATAATCTTCGTCGTAGAACATCGCTTCGTCGTCACCTGCATCTTTc > 1:2085231/1‑69 (MQ=255) aaGCCATGTTCCAGTGCGGTGACATAATCTTCGTCGTAGAACATCGCTTCGTCGTCACCTGCATCTTTc > 1:636574/1‑69 (MQ=255) aaGCCATGTTCCAGTGCGGTGACATAATCTTCGTCGTAGAACATCGCTTCGTCGTCACCTGCATCTTTc > 1:581044/1‑69 (MQ=255) aaGCCATGTTCCAGTGCGGTGACATAATCTTCGTCGTAGAACATCGCTTCGTCGTCACCTGCATCTTTc > 1:434352/1‑69 (MQ=255) aaGCCATGTTCCAGTGCGGTGACATAATCTTCGTCGTAGAACATCGCTTCGTCGTCACCTGCATCTTTc > 1:41781/1‑69 (MQ=255) aaGCCATGTTCCAGTGCGGTGACATAATCTTCGTCGTAGAACATCGCTTCGTCGTCACCTGCATCTTTc > 1:512542/1‑69 (MQ=255) aaGCCATGTTCCAGTGCGGTGACATAATCTTCGTCGTAGAACATCGCTTCGTCGTCACCTGCATCTTTc > 1:3024957/1‑69 (MQ=255) aaGCCATGTTCCAGTGCGGTGACATAATCTTCGTCGTAGAACATCGCTTCGTCGTCACCTGCATCTTTc > 1:2786431/1‑69 (MQ=255) aaGCCATGTTCCAGTGCGGTGACATAATCTTCGTCGTAGAACATCGCTTCGTCGTCACCTGCATCTTTc > 1:873809/1‑69 (MQ=255) aaGCCATGTTCCAGTGCGGTGACATAATCTTCGTCGTAGAACATCGCTTCGTCGTCACCTGCATCTTTc > 1:753182/1‑69 (MQ=255) aaGCCATGTTCCAGTGCGGTGACATAATCTTCGTCGTAGAACATCGCTTCGTCGTCACCTGCATCTTTc > 1:1165925/1‑69 (MQ=255) aaGCCATGTTCCAGTGCGGTGACATAATCTTCGTCGTAGAACATCGCTTCGTCGTCACCTGCATCTTTc > 1:1204466/1‑69 (MQ=255) tAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGc > 1:1169678/1‑67 (MQ=255) tAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCg > 1:2234098/1‑68 (MQ=255) tAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCg > 1:764288/1‑68 (MQ=255) tAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGt > 1:2964369/1‑69 (MQ=255) tAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGt > 1:204113/1‑69 (MQ=255) tAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGt > 1:2793848/1‑69 (MQ=255) tAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGt > 1:1725792/1‑69 (MQ=255) ttCGTCGTAGAACATCGCTTCGTCGTCACCTGCATc > 1:1213683/1‑36 (MQ=37) ttCGTCGTAGAACATCGCTTCGTCGTCACCTGCATc > 1:1370849/1‑36 (MQ=37) ttCGTCGTAGAACATCGCTTCGTCGTCACCTGCATc > 1:2496397/1‑36 (MQ=37) ttCGTCGTAGAACATCGCTTCGTCGTCACCTGCATc > 1:3057330/1‑36 (MQ=37) ttCGTCGTAGAACATCGCTTCGTCGTCACCTGCATc > 1:3120184/1‑36 (MQ=37) ttCGTCGTAGAACATCGCTTCGTCGTCACCTGCATc > 1:3124134/1‑36 (MQ=37) ttCGTCGTAGAACATCGCTTCGTCGTCACCTGCATc > 1:3141190/1‑36 (MQ=37) ttCGTCGTAGAACATCGCTTCGTCGTCACCTGCATc > 1:2266436/1‑36 (MQ=37) ttCGTCGTAGAACATCGCTTCGTCGTCACCTGCATc > 1:2929368/1‑36 (MQ=37) ttCGTCGTAGAACATCGCTTCGTCGTCACCTGCATc > 1:617395/1‑36 (MQ=37) ttCGTCGTAGAACATCGCTTCGTCGTCACCTGCATc > 1:279791/1‑36 (MQ=37) ttCGTCGTAGAACATCGCTTCGTCGTCACCTGCATc > 1:3293645/1‑36 (MQ=37) ttCGTCGTAGAACATCGCTTCGTCGTCACCTGCATc > 1:396064/1‑36 (MQ=37) ttCGTCGTAGAACATCGCTTCGTCGTCACCTGCATc > 1:211168/1‑36 (MQ=37) ttCGTCGTAGAACATCGCTTCGTCGTCACCTGCATc > 1:5759/1‑36 (MQ=37) ttCGTCGTAGAACATCGCTTCGTCGTCACCTGCATc > 1:1734180/1‑36 (MQ=37) ttCGTCGTAGAACATCGCTTCGTCGTCACCTGCATc > 1:1246931/1‑36 (MQ=37) ttCGTCGTAGAACATCGCTCCGTCGTCACCTGCATc > 1:1020715/1‑36 (MQ=255) tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCGTTAACCTGTTCCTGGAAACGTTCAGCCt < 1:979700/64‑1 (MQ=255) tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCGTTAACCTGTTCCTGGAAACGTTCAGCCt < 1:883767/64‑1 (MQ=255) tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCGTTAACCTGTTCCTGGAAACGTTCAGCCt < 1:3233087/64‑1 (MQ=255) tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCGTTAACCTGTTCCTGGAAACGTTCAGCCt < 1:2832559/64‑1 (MQ=255) tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCGTTAACCTGTTCCTGGAAACGTTCAGCCt < 1:820518/64‑1 (MQ=255) tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCGTTAACCTGTTCCTGGAAACGTTCAGCCt < 1:2751161/64‑1 (MQ=255) tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCGTTAACCTGTTCCTGGAAACGTTCAGCCt < 1:713601/64‑1 (MQ=255) tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCGTTAACCTGTTCCTGGAAACGTTCAGCCt < 1:113725/64‑1 (MQ=255) tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCGTTAACCTGTTCCTGGAAACGTTCAGCCt < 1:1317118/64‑1 (MQ=255) tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCGTTAACCTGTTCCTGGAAACGTTCAGCCt < 1:2253816/64‑1 (MQ=255) tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCGTTAACCTGTTCCTGGAAACGTTCAGCCt < 1:2397591/64‑1 (MQ=255) tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCGTTAACCTGTTCCTGGAAACGTTCAGCCt < 1:2559669/64‑1 (MQ=255) tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCGTTAACCTGTTCCTGGAAACGTTCAGCCt < 1:1978480/64‑1 (MQ=255) tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCGTTAACCTGTTCCTGGAAACGTTCAGCCt < 1:2645863/64‑1 (MQ=255) tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCGTTAACCTGTTCCTGGAAACGTTCAGCCt < 1:1727329/64‑1 (MQ=255) tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCGTTAACCTGTTCCTGGAAACGTTCAGCCt < 1:1533129/64‑1 (MQ=255) tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCCTTAACCTGTTCCTGGAAACGTTCAGCCt < 1:1113009/64‑1 (MQ=255) tcatAGAACATGGCTTAGTCGTCACCTGCAGCTTTAGCGTTAACCTGTTCCTGGAAACGTTCAGCCt < 1:2325090/64‑1 (MQ=255) tCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg < 1:1341223/68‑1 (MQ=255) tCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg < 1:647508/68‑1 (MQ=255) tCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg < 1:1464107/68‑1 (MQ=255) tCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg < 1:2056828/68‑1 (MQ=255) tCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg < 1:422902/68‑1 (MQ=255) tCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg < 1:206441/68‑1 (MQ=255) tCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg < 1:1561438/68‑1 (MQ=255) tCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg < 1:397867/68‑1 (MQ=255) tCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg < 1:2125301/68‑1 (MQ=255) tCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg < 1:1305331/68‑1 (MQ=255) tCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg < 1:3116184/68‑1 (MQ=255) tCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg < 1:2457750/68‑1 (MQ=255) tCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg < 1:2985568/68‑1 (MQ=255) tCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg < 1:2636796/68‑1 (MQ=255) tCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg < 1:909244/68‑1 (MQ=255) tCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg < 1:107435/68‑1 (MQ=255) catAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCGTTAACCTGTTCCTGGAAACGTTCAGCCt < 1:3237063/64‑1 (MQ=255) cGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg < 1:66202/67‑1 (MQ=255) cGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGAt < 1:1292710/69‑1 (MQ=255) cGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGAt < 1:1159043/69‑1 (MQ=255) cGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGAt < 1:2754818/69‑1 (MQ=255) cGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGAt < 1:983141/69‑1 (MQ=255) cGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGAt < 1:2595544/69‑1 (MQ=255) cGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGAt < 1:2927896/69‑1 (MQ=255) gCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCa > 1:1954060/1‑40 (MQ=255) gCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCa > 1:2449204/1‑40 (MQ=255) ttCGTCGTCACCTGCATCTTTCGCGGCAACCTGATCCAGGAAGCGCTGCGCCTGATCTTCCGCGTCAtt < 1:453795/69‑1 (MQ=255) ttCGTCGTCACCTGCATCTTTCGCGGCAACCTGATCCAGGAAGCGCTGCGCCTGATCTTCCGCGTCAtt < 1:3346930/69‑1 (MQ=255) ttCGTCGTCACCTGCATCTTTCGCGGCAACCTGATCCAGGAAGCGCTGCGCCTGATCTTCCGCGTCAtt < 1:711680/69‑1 (MQ=255) ttCGTCGTCACCTGCATCTTTCGCGGCAACCTGATCCAGGAAGCGCTGCGCCTGATCTTCCGCGTCAtt < 1:3318502/69‑1 (MQ=255) ttCGTCGTCACCTGCATCTTTCGCGGCAACCTGATCCAGGAAGCGCTGCGCCTGATCTTCCGCGTCAtt < 1:2151875/69‑1 (MQ=255) ttCGTCGTCACCTGCATCTTTCGCGGCAACCTGATCCAGGAAGCGCTGCGCCTGATCTTCCGCGTCAtt < 1:3072501/69‑1 (MQ=255) ttCGTCGTCACCTGCATCTTTCGCGGCAACCTGATCCAGGAAGCGCTGCGCCTGATCTTCCGCGTCAtt < 1:1023612/69‑1 (MQ=255) tcgtcgTCACCTGCATCTTTCGCGGCAACCTGATCCAGGAAGCGCTGCGCCTGATCTTCCGCGTCAtt < 1:3150443/68‑1 (MQ=255) tcgtcgTCACCTGCATCTTTCGCGGCAACCTGATCCAGGAAGCGCTGCGCCTGATCTTCCGCGTCAtt < 1:272808/68‑1 (MQ=255) tGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTaaa > 1:1000987/1‑69 (MQ=255) | GGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAA > minE/1933031‑1933164 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |