| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,934,611 | G→A | 41.5% | N288N (AAC→AAT) | prfB ← | peptide chain release factor RF‑2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,934,611 | 0 | G | A | 41.5% | 49.4 / 86.1 | 118 | N288N (AAC→AAT) | prfB | peptide chain release factor RF‑2 |
| Reads supporting (aligned to +/- strand): ref base G (20/49); new base A (32/17); total (52/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.44e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.24e-01 | |||||||||||
Only 100 of 118 total aligned reads displayed.
GATCTTTAATGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTT > minE/1934544‑1934676 | gATCTTTAATGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGt < 1:289742/69‑1 (MQ=255) aTCTTTAATGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGtt < 1:2443414/69‑1 (MQ=255) aTCTTTAATGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGtt < 1:791873/69‑1 (MQ=255) aTCTTTAATGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGtt < 1:2561382/69‑1 (MQ=255) aTCTTTAATGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGtt < 1:2430491/69‑1 (MQ=255) aTCTTTAATGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGtt < 1:1547629/69‑1 (MQ=255) aTCTTTAATGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGtt < 1:2035899/69‑1 (MQ=255) aTCTTTAATGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGtt < 1:2031152/69‑1 (MQ=255) aTCTTTAATGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGt < 1:1018649/68‑1 (MQ=255) aTCTTTAATGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTCCCCCAGCCGATGACGGATTTGtt < 1:1549172/69‑1 (MQ=255) tCTTTAATGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTa < 1:1186300/69‑1 (MQ=255) aTGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATc < 1:3150433/65‑1 (MQ=255) aTGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATc < 1:2453742/65‑1 (MQ=255) aTGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATc < 1:1984329/65‑1 (MQ=255) aTGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATc < 1:3328264/65‑1 (MQ=255) aTGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATc < 1:501700/65‑1 (MQ=255) gAGTCATCAAGGACATAAGAACGTATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGcc < 1:143685/69‑1 (MQ=255) gAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGcc < 1:1468851/69‑1 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCCGCCGATGTCGGATTTGTTATCTTcc > 1:2752585/1‑58 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATTTCGGATTTATTGTCTTCCATCGCCTGttt > 1:829365/1‑69 (MQ=38) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTcc > 1:2012054/1‑58 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTcc > 1:2356865/1‑58 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTcc > 1:2516551/1‑58 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTcc > 1:986743/1‑58 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:2346226/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:989974/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:247824/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:2516617/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:2517886/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:2775622/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:3178220/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:3213361/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:436059/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:546748/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:61427/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:1014821/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:147928/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:2167385/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGtt > 1:796446/1‑68 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGtt > 1:600255/1‑68 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCATCCATCGCCTGttt > 1:2701666/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCAGATGTCGGATTTGTTATCTTcc > 1:3149678/1‑58 (MQ=255) aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGt < 1:2234962/47‑1 (MQ=255) aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGt < 1:1405709/47‑1 (MQ=255) aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGt < 1:1394475/47‑1 (MQ=255) aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGt < 1:3289259/47‑1 (MQ=255) aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGt < 1:3336762/47‑1 (MQ=255) aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGt < 1:3422950/47‑1 (MQ=255) aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGt < 1:486636/47‑1 (MQ=255) aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGt < 1:699428/47‑1 (MQ=255) aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGt < 1:2296791/47‑1 (MQ=255) aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGt < 1:747916/47‑1 (MQ=255) aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTa > 1:397533/1‑49 (MQ=255) aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTa > 1:1206551/1‑49 (MQ=255) aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTa > 1:2603170/1‑49 (MQ=255) aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTtctc > 1:178429/1‑69 (MQ=255) aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTtctc > 1:2070329/1‑69 (MQ=255) aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTtctc > 1:3127816/1‑69 (MQ=255) ggACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCgg < 1:854292/69‑1 (MQ=255) ggACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCgg < 1:1507202/69‑1 (MQ=255) tAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGtt < 1:3172864/41‑1 (MQ=255) aGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCAttttt > 1:2219956/1‑69 (MQ=255) aGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCAttttt > 1:3337018/1‑69 (MQ=255) aaCGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTttct < 1:926240/69‑1 (MQ=255) aaCGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTttct < 1:1672037/69‑1 (MQ=255) gCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAg < 1:2828619/69‑1 (MQ=255) gCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAg < 1:2306254/69‑1 (MQ=255) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTtctc > 1:1817393/1‑37 (MQ=18) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTtct > 1:1087597/1‑36 (MQ=18) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCTGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:1224988/1‑69 (MQ=38) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:1111457/1‑69 (MQ=255) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:784018/1‑69 (MQ=255) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:2293531/1‑69 (MQ=255) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:2223796/1‑69 (MQ=255) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:1249445/1‑69 (MQ=255) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:3259329/1‑69 (MQ=255) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:74227/1‑69 (MQ=255) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:1884385/1‑69 (MQ=255) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:2868757/1‑69 (MQ=255) tCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTc > 1:443924/1‑69 (MQ=255) tCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTc > 1:2751105/1‑69 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATGTCCAGTTCATATAGCTTCGCtt < 1:2531440/69‑1 (MQ=38) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:1871262/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:2208936/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:2779254/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:2755036/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:2284600/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:1629754/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:3173556/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:1240247/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:629759/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:1156135/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:2084547/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:1161119/69‑1 (MQ=255) tttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCttt < 1:1762128/69‑1 (MQ=255) tttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCttt < 1:1871763/69‑1 (MQ=255) tttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCttt < 1:855392/69‑1 (MQ=255) tttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCttt < 1:310302/69‑1 (MQ=255) tttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCttt < 1:2472880/69‑1 (MQ=255) tttATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:1000157/68‑1 (MQ=255) | GATCTTTAATGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTT > minE/1934544‑1934676 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |