| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,934,614 | A→G | 55.7% | D287D (GAT→GAC) | prfB ← | peptide chain release factor RF‑2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,934,614 | 0 | A | G | 55.7% | 20.6 / 70.1 | 88 | D287D (GAT→GAC) | prfB | peptide chain release factor RF‑2 |
| Reads supporting (aligned to +/- strand): ref base A (16/23); new base G (32/17); total (48/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.14e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 | |||||||||||
TCTTTAATGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTT > minE/1934546‑1934676 | tCTTTAATGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTa < 1:1186300/69‑1 (MQ=255) aTGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATc < 1:2505047/65‑1 (MQ=255) aTGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATc < 1:1984329/65‑1 (MQ=255) aTGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATc < 1:3150433/65‑1 (MQ=255) aTGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATc < 1:501700/65‑1 (MQ=255) aTGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATc < 1:3328264/65‑1 (MQ=255) aTGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATc < 1:2453742/65‑1 (MQ=255) gAGTCATCAAGGACATAAGAACGTATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGcc < 1:143685/69‑1 (MQ=255) gAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGcc < 1:1468851/69‑1 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCCGCCGATGTCGGATTTGTTATCTTcc > 1:2752585/1‑58 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATTTCGGATTTATTGTCTTCCATCGCCTGttt > 1:829365/1‑69 (MQ=38) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTcc > 1:2516551/1‑58 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTcc > 1:2356865/1‑58 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTcc > 1:1159194/1‑58 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTcc > 1:986743/1‑58 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTcc > 1:2012054/1‑58 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:2346226/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:2167385/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:247824/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:3178220/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:2516617/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:2517886/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:2775622/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:1014821/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:3213361/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:3241470/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:436059/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:546748/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:61427/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:989974/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGttt > 1:147928/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGtt > 1:796446/1‑68 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCTTCCATCGCCTGtt > 1:600255/1‑68 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTATTGTCATCCATCGCCTGttt > 1:2701666/1‑69 (MQ=255) atcaAGGACATAAGAACGAATCTGGCTGCCCCAGCAGATGTCGGATTTGTTATCTTcc > 1:3149678/1‑58 (MQ=255) aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTa > 1:2603170/1‑49 (MQ=255) aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTa > 1:3399203/1‑49 (MQ=255) aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTa > 1:397533/1‑49 (MQ=255) aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTa > 1:1206551/1‑49 (MQ=255) aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTtctc > 1:178429/1‑69 (MQ=255) aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTtctc > 1:3127816/1‑69 (MQ=255) aaGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTtctc > 1:2070329/1‑69 (MQ=255) ggACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCgg < 1:2781954/69‑1 (MQ=255) ggACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCgg < 1:1507202/69‑1 (MQ=255) ggACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCgg < 1:854292/69‑1 (MQ=255) aGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCAttttt > 1:2219956/1‑69 (MQ=255) aGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCAttttt > 1:3337018/1‑69 (MQ=255) aGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCAttttt > 1:2691763/1‑69 (MQ=255) aaCGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTttct < 1:926240/69‑1 (MQ=255) aaCGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTttct < 1:1672037/69‑1 (MQ=255) gCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAg < 1:2306254/69‑1 (MQ=255) gCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAg < 1:2828619/69‑1 (MQ=255) gCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAg < 1:1947593/69‑1 (MQ=255) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTtctc > 1:1817393/1‑37 (MQ=18) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTtct > 1:1087597/1‑36 (MQ=18) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCTGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:1224988/1‑69 (MQ=38) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:1111457/1‑69 (MQ=255) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:3259329/1‑69 (MQ=255) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:1249445/1‑69 (MQ=255) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:784018/1‑69 (MQ=255) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:2868757/1‑69 (MQ=255) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:74227/1‑69 (MQ=255) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:997813/1‑69 (MQ=255) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:2223796/1‑69 (MQ=255) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:2293531/1‑69 (MQ=255) cGATGTCGGATTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATaaa > 1:1884385/1‑69 (MQ=255) tCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTc > 1:2751105/1‑69 (MQ=255) tCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTc > 1:443924/1‑69 (MQ=255) ggATTTGTTATCTTCCATCGCCTGTTTCTCGGCAttttt > 1:2253456/1‑39 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATGTCCAGTTCATATAGCTTCGCtt < 1:2531440/69‑1 (MQ=38) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:1871262/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:2084547/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:2208936/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:2284600/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:629759/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:1156135/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:1161119/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:178065/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:1240247/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:1629754/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:1324707/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:2755036/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:3173556/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:2779254/69‑1 (MQ=255) aTTTATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:3129970/69‑1 (MQ=255) tttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCttt < 1:2472880/69‑1 (MQ=255) tttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCttt < 1:310302/69‑1 (MQ=255) tttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCttt < 1:1871763/69‑1 (MQ=255) tttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCttt < 1:1762128/69‑1 (MQ=255) tttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCttt < 1:855392/69‑1 (MQ=255) tttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCttt < 1:2117296/69‑1 (MQ=255) tttATTGTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCtt < 1:1000157/68‑1 (MQ=255) ttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCttt < 1:1487685/68‑1 (MQ=255) | TCTTTAATGCGGGAGTCATCAAGGACATAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTT > minE/1934546‑1934676 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |