| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,936,247 | G→A | 58.7% | R376R (CGC→CGT) | recJ ← | ssDNA exonuclease, 5' ‑‑> 3'‑specific |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,936,247 | 0 | G | A | 58.7% | 25.5 / 53.6 | 75 | R376R (CGC→CGT) | recJ | ssDNA exonuclease, 5' ‑‑> 3'‑specific |
| Reads supporting (aligned to +/- strand): ref base G (27/4); new base A (15/29); total (42/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCC > minE/1936181‑1936305 | aCCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATgc > 1:215038/1‑68 (MQ=255) cAGCGTACCGTCGCCCGCAGGCGCAAAGGCGATAACCGGACGGTGAAAACGCTCTTTGATACGCGat > 1:1058974/1‑66 (MQ=255) cAGCGTACCGTCGCCCGCAGGCGCAAAGGCGATAACCGGACGGTGAAAACGCTCTTTGATACGCGat > 1:925532/1‑66 (MQ=255) cAGCGTACCGTCGCCCGCAGGCGCAAAGGCGATAACCGGACGGTGAAAACGCTCTTTGATACGCGat > 1:784069/1‑66 (MQ=255) cAGCGTACCGTCGCCCGCAGGCGCAAAGGCGATAACCGGACGGTGAAAACGCTCTTTGATACGCGat > 1:3379977/1‑66 (MQ=255) cAGCGTACCGTCGCCCGCAGGCGCAAAGGCGATAACCGGACGGTGAAAACGCTCTTTGATACGCGat > 1:2561897/1‑66 (MQ=255) cAGCGTACCGTCGCCCGCAGGCGCAAAGGCGATAACCGGACGGTGAAAACGCTCTTTGATACGCGat > 1:2434772/1‑66 (MQ=255) cAGCGTACCGTCGCCCGCAGGCGCAAAGGCGATAACCGGACGGTGAAAACGCTCTTTGATACGCGat > 1:2313532/1‑66 (MQ=255) cAGCGTACCGTCGCCCGCAGGCGCAAAGGCGATAACCGGACGGTGAAAACGCTCTTTGATACGCGat > 1:1937056/1‑66 (MQ=255) cAGCGTACCGTCGCCCGCAGGCGCAAAGGCGATAACCGGACGGTGAAAACGCTCTTTGATACGCGat > 1:1930865/1‑66 (MQ=255) cAGCGTACCGTCGCCCGCAGGCGCAAAGGCGATAACCGGACGGTGAAAACGCTCTTTGATACGCGat > 1:1885089/1‑66 (MQ=255) cAGCGTACCGTCGCCCGCAGGCGCAAAGGCGATAACCGGACGGTGAAAACGCTCTTTGATACGCGat > 1:1746966/1‑66 (MQ=255) cAGCGTACCGTCGCCCGCAGGCGCAAAGGCGATAACCGGACGGTGAAAACGCTCTTTGATACGCGat > 1:1010619/1‑66 (MQ=255) cAGCGTACCGTCGCCCGCAGGCGCAAAGGCGATAACCGGACGGTGAAAACGCTCTTTGATACGCGat > 1:1111433/1‑66 (MQ=255) cAGCGTACCGTCGCCCGCAGGCGCAAAGGCGATAACCGGACGGTGAAAACGCTCTTTGATACGCGat > 1:1614508/1‑66 (MQ=255) cAGCGTACCGTCGCCCGCAGGCGCAAAGGCGATAACCGGACGGTGAAAACGCTCTTTGATACGCGat > 1:1521854/1‑66 (MQ=255) ccGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg > 1:45203/1‑65 (MQ=255) ccGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg > 1:2974404/1‑65 (MQ=255) ccGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg > 1:3090993/1‑65 (MQ=255) ccGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg > 1:1206938/1‑65 (MQ=255) ccGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg > 1:2380234/1‑65 (MQ=255) ccGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg > 1:2336510/1‑65 (MQ=255) ccGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg > 1:110201/1‑65 (MQ=255) ccGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg > 1:2077410/1‑65 (MQ=255) ccGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg > 1:2064816/1‑65 (MQ=255) ccGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg > 1:2017489/1‑65 (MQ=255) ccGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg > 1:1080077/1‑65 (MQ=255) ccGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg > 1:1378882/1‑65 (MQ=255) ccGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg > 1:906538/1‑65 (MQ=255) aCCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg > 1:674059/1‑60 (MQ=255) aCCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg > 1:3076974/1‑60 (MQ=255) aCCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg > 1:979411/1‑60 (MQ=255) aCCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg > 1:362802/1‑60 (MQ=255) aCCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg > 1:3128344/1‑60 (MQ=255) aCCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg > 1:2346232/1‑60 (MQ=255) aCCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg > 1:3322314/1‑60 (MQ=255) aCCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAg > 1:3212865/1‑60 (MQ=255) gcAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCt < 1:1693717/67‑1 (MQ=255) cGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATAc < 1:892725/69‑1 (MQ=255) tGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACAtt < 1:33155/69‑1 (MQ=255) tGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACAtt < 1:46611/69‑1 (MQ=255) aaaaCGCTCTTTGATACGCGATGCCAGAATGCCAACAATGCCCTGATGCCATTCAGGGTGATACa < 1:3226067/65‑1 (MQ=255) aaaaCGCTCTTTGATACGCGATGCCAGAATGCCAACAATGCCCTGATGCCATTCAGGGTGATACa < 1:3394312/65‑1 (MQ=255) aaaaCGCTCTTTGATACGCGATGCCAGAATGCCAACAATGCCCTGATGCCATTCAGGGTGATACa < 1:2757499/65‑1 (MQ=255) aaaaCGCTCTTTGATACGCGATGCCAGAATGCCAACAATGCCCTGATGCCATTCAGGGTGATACa < 1:466495/65‑1 (MQ=255) aaaaCGCTCTTTGATACGCGATGCCAGAATGCCAACAATGCCCTGATGCCATTCAGGGTGATACa < 1:2810742/65‑1 (MQ=255) aaaaCGCTCTTTGATACGCGATGCCAGAATGCCAACAATGCCCTGATGCCATTCAGGGTGATACa < 1:1854574/65‑1 (MQ=255) aaaaCGCTCTTTGATACGCGATGCCAGAATGCCAACAATGCCCTGATGCCATTCAGGGTGATACa < 1:1827457/65‑1 (MQ=255) aaaaCGCTCTTTGATACGCGATGCCAGAATGCCAACAATGCCCTGATGCCATTCAGGGTGATACa < 1:1809288/65‑1 (MQ=255) aaaaCGCTCTTTGATACGCGATGCCAGAATGCCAACAATGCCCTGATGCCATTCAGGGTGATACa < 1:2101853/65‑1 (MQ=255) aaaaCGCTCTTTGATACGCGATGCCAGAATGCCAACAATGCCCTGATGCCATTCAGGGTGATACa < 1:1263529/65‑1 (MQ=255) aaaaCGCTCTTTGATACGCGATGCCAGAATGCCAACAATGCCCTGATGCCATTCAGGGTGATACa < 1:2220417/65‑1 (MQ=255) aaaaCGCTCTTTGATACGCGATGCCAGAATGCCAACAATGCCCTGATGCCATTCAGGGTGATACa < 1:1771586/65‑1 (MQ=255) aaaaCGCTCTTTGATACGCGATGCCAGAATGCCAACAATGCCCTGATGCCATTCAGGGTGATACa < 1:2405406/65‑1 (MQ=255) aaaaCGCTCTTTGATACGCGATGCCAGAATGCCAACAATGCCCTGATGCCATTCAGGGTGATACa < 1:2579207/65‑1 (MQ=255) aaaaCGCTCTTTGATACGCGATGCCAGAATGCCAACAATGCCCTGATGCCATTCAGGGTGATACa < 1:2734859/65‑1 (MQ=255) aaaaCGCTCTTTGATACGCGATGCCAGAATGCCAACAATGCCCTGATGCCATTCAGGGTGATACa < 1:1710882/65‑1 (MQ=255) aaaaCGCTCTTTGATACGCGATGCAAGAATGCCAACAATGCCCTGATGCCATTCAGGGTGATACa < 1:574772/65‑1 (MQ=255) aaaCGCTCTTTGATACGCGATGCCAGAATGCCAACACTGCCCTGATGCCATTCAGGGTGATACa < 1:2666724/64‑1 (MQ=255) aaaCGCTCTTTGATACGCGATGCCAGAATGCCAACAATGCCCTGATGCCATTCAGGGTGATACa < 1:3337728/64‑1 (MQ=255) aaCGCTCTTTGATACGCGATGCCAGAATGCCAACAATGCCCTGATGCCATTCAGGGTGATACa < 1:2526623/63‑1 (MQ=255) aaCGCTCTTTGATACGCGATGCCAGAATGCCAACAACGCCCTGATGCCATTCAGGGTGATACATTGCCa < 1:266200/69‑1 (MQ=255) aaCGCTCTTTGATACGCGATGCCAGAATGCCAACAACGCCCTGATGCCATTCAGGGTGATACATTGCCa < 1:2250229/69‑1 (MQ=255) aaCGCTCTTTGATACGCGATGCCAGAATGCCAACAACGCCCTGATGCCATTCAGGGTGATACATTGCCa < 1:3201266/69‑1 (MQ=255) aaCGCTCTTTGATACGCGATGCCAGAATGCCAACAACGCCCTGATGCCATTCAGGGTGATACATTGCCa < 1:461993/69‑1 (MQ=255) aaCGCTCTTTGATACGCGATGCCAGAATGCCAACAACGCCCTGATGCCATTCAGGGTGATACATTGCCa < 1:2756445/69‑1 (MQ=255) aaCGCTCTTTGATACGCGATGCCAGAATGCCAACAACGCCCTGATGCCATTCAGGGTGATACATTGCCa < 1:2058694/69‑1 (MQ=255) aCGCTCTTTGATACGCGATGCCAGAATGCCAACAACGCCCTGATGCCATTCAGGGTGATACATTGCCa < 1:3206759/68‑1 (MQ=255) aCGCTCTTTGATACGCGATGCCAGAATGCCAACAACGCCCTGATGCCATTCAGGGTGATACATTGCCa < 1:2925541/68‑1 (MQ=255) cgcgCTTTGATACGCGATGCCAGAATGCCAACAACGCCCTGATGCCATTCAGGGTGATACATTGCCa < 1:1248924/67‑1 (MQ=255) gCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGcc > 1:982115/1‑69 (MQ=255) gCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGcc > 1:1986778/1‑69 (MQ=255) gCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGcc > 1:666719/1‑69 (MQ=255) gCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGcc > 1:206632/1‑69 (MQ=255) gCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGcc > 1:2851254/1‑69 (MQ=255) gCTATTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGcc > 1:344011/1‑69 (MQ=255) | ACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCC > minE/1936181‑1936305 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |