| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,938,229 | T→C | 32.7% | L261L (TTA→TTG) | xerD ← | site‑specific tyrosine recombinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,938,229 | 0 | T | C | 32.7% | 101.4 / 67.9 | 125 | L261L (TTA→TTG) | xerD | site‑specific tyrosine recombinase |
| Reads supporting (aligned to +/- strand): ref base T (54/30); new base C (14/27); total (68/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.07e-01 | |||||||||||
Only 100 of 125 total aligned reads displayed.
CGTATAAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGT > minE/1938172‑1938286 | cGTATAAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCAcc > 1:1570067/1‑69 (MQ=255)cGTATAAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCAcc > 1:2203503/1‑69 (MQ=255)cGTATAAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCAcc > 1:2210052/1‑69 (MQ=255)cGTATAAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCAcc > 1:2836886/1‑69 (MQ=255)cGTATAAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCAcc > 1:13634/1‑69 (MQ=255)cGTATAAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCAcc > 1:732377/1‑69 (MQ=255)cGTATAAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCAcc > 1:92644/1‑69 (MQ=255)cGTATAAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCAcc > 1:807064/1‑69 (MQ=255)cGTATAAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCAc > 1:908433/1‑68 (MQ=255) taAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGa > 1:736507/1‑69 (MQ=255) taAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGa > 1:2088949/1‑69 (MQ=255) taAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGa > 1:1078529/1‑69 (MQ=255) taAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGa > 1:2313202/1‑69 (MQ=255) taAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGa > 1:2647486/1‑69 (MQ=255) taAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGa > 1:2732975/1‑69 (MQ=255) taAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGa > 1:2826969/1‑69 (MQ=255) taAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGa > 1:1853113/1‑69 (MQ=255) taAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGa > 1:2842972/1‑69 (MQ=255) taAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGa > 1:73955/1‑69 (MQ=255) taAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGa > 1:1554602/1‑69 (MQ=255) taAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCAGCACCATGa > 1:2076515/1‑69 (MQ=255) aaTTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGAt > 1:3421139/1‑68 (MQ=255) aaTTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGAt > 1:2865060/1‑68 (MQ=255) cGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTtaata < 1:3247830/68‑1 (MQ=255) cGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTtaata < 1:3046135/68‑1 (MQ=255) cGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTtaata < 1:698280/68‑1 (MQ=255) cGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTtaata < 1:3160934/68‑1 (MQ=255) cGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTtaata < 1:1234869/68‑1 (MQ=255) tggtggAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAAt < 1:108278/69‑1 (MQ=255) tggtggAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAAt < 1:1852875/69‑1 (MQ=255) tggtggAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAAt < 1:3229210/69‑1 (MQ=255) ggtggAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATg < 1:357488/69‑1 (MQ=255) cGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAg > 1:283882/1‑69 (MQ=255) cGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAg > 1:3431638/1‑69 (MQ=255) cGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAg > 1:2568357/1‑69 (MQ=255) cGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAg > 1:428605/1‑69 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACTCa < 1:502807/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:2184208/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:904251/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:862896/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:82827/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:1140483/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:1222736/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:599293/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:1353057/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:1422129/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:3383325/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:3339364/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:3314084/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:2997469/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:2992552/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:295012/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:1704433/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:1792729/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:2032157/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:2462475/69‑1 (MQ=255) gTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca < 1:1990500/69‑1 (MQ=255) gTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca < 1:1971454/69‑1 (MQ=255) gCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACAt > 1:1703390/1‑69 (MQ=255) gCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACAt > 1:1646881/1‑69 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGa > 1:1319464/1‑54 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGa > 1:465949/1‑54 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGa > 1:486450/1‑54 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGa > 1:523821/1‑54 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGa > 1:1239542/1‑54 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGa > 1:3223931/1‑54 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGa > 1:2383447/1‑54 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGa > 1:807148/1‑54 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca < 1:666335/61‑1 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca < 1:1176957/61‑1 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca < 1:1991035/61‑1 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca < 1:808725/61‑1 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca < 1:2063796/61‑1 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca < 1:2112318/61‑1 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca > 1:1059824/1‑61 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca < 1:2915340/61‑1 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca > 1:102379/1‑61 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca > 1:1360913/1‑61 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca < 1:3138009/61‑1 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca > 1:1691544/1‑61 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca > 1:1565183/1‑61 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTAGCAAAAGCGTGa > 1:1157842/1‑54 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAAAGAGTGGCAAAAg > 1:1127597/1‑49 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:690640/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:2116239/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:3026316/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:3171997/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:449692/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:669749/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:3267203/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:1861574/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:588904/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:3335642/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:2787190/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:476440/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:292392/1‑69 (MQ=255) ccacGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGt < 1:3193060/65‑1 (MQ=255) ccacGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGt < 1:1711552/65‑1 (MQ=255) ccacGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGt < 1:747935/65‑1 (MQ=255) gTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTCACGCAaca < 1:2644274/51‑1 (MQ=255) | CGTATAAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGT > minE/1938172‑1938286 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |